通过基因沉默剖析粘附毛原虫的肽能信号通路。

IF 7.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Current Biology Pub Date : 2025-09-22 Epub Date: 2025-08-27 DOI:10.1016/j.cub.2025.07.077
Wanqing Li, Minjun Jin, Muyang Ren, Qiuyao Guo, Xiaofei Lu, Meng Qiu, Bo Dong, Chengtian Zhao
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引用次数: 0

摘要

黏着毛滴虫是一种简单的多细胞海洋生物,属于盘虫门,1,2是最基础的后生动物谱系之一,与栉虫、Porifera和刺胞虫齐名。3,4,5由于其非常简单的身体结构,6,7为多细胞生物的进化提供了有价值的见解8,9有趣的是,尽管缺乏真正的组织和器官,毛原虫已经进化出多种细胞类型,可以执行协调,复杂的行为,包括进食肽能信号传导参与了这些行为的调控,14,15,16,17,18,19,尽管其潜在机制尚不清楚。研究这些过程的一个主要挑战是缺乏针对这种生物体的既定遗传操作方法。在这项研究中,我们开发了一种有效的基因敲低技术,使用二氧化硅纳米颗粒介导的RNA干扰(RNAi),20,21,22,23,与传统的转染方法相比,显示出更高的效率和生物安全性。利用这种方法,我们确认了两个纤毛基因在纤毛发生中的作用,并确定了一个经典神经肽在调节摄食行为中的功能。此外,分子动力学模拟和敲除实验揭示了这种神经肽的受体,为这种基础后生动物的细胞运动和摄食协调提供了新的见解。我们的发现提出了一种新的有效的毛虫基因敲除策略,提高了海洋模型中基因操作的潜力,增强了我们对placozoan生物学的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dissecting a peptidergic signaling pathway in Trichoplax adhaerens by gene silencing.

Trichoplax adhaerens, a simple multicellular marine organism from the phylum Placozoa,1,2 is one of the most basal metazoan lineages, alongside Ctenophora, Porifera, and Cnidaria.3,4,5 With its remarkably simple body plan,6,7Trichoplax provides valuable insights into the evolution of multicellularity.8,9 Interestingly, despite lacking true tissues and organs, Trichoplax has evolved multiple cell types that can perform coordinated, complex behaviors, including feeding.10,11,12,13 Peptidergic signaling has been implicated in the regulation of such behaviors,14,15,16,17,18,19 though the underlying mechanisms remain poorly understood. A major challenge in investigating these processes has been the lack of established genetic manipulation methods for this organism. In this study, we developed an effective gene knockdown technique using silica nanoparticle-mediated RNA interference (RNAi),20,21,22,23 which demonstrates superior efficiency and biosafety compared with traditional transfection methods. Using this approach, we confirmed the roles of two ciliary genes in ciliogenesis and identified the function of a classic neuropeptide in regulating feeding behavior. Furthermore, molecular dynamics simulations and knockdown experiments revealed the receptor for this neuropeptide, providing new insights into the coordination of cell movement and feeding in this basal metazoan. Our findings present a novel and effective gene knockdown strategy for Trichoplax, advancing the potential for gene manipulation in marine models and enhancing our understanding of placozoan biology.

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来源期刊
Current Biology
Current Biology 生物-生化与分子生物学
CiteScore
11.80
自引率
2.20%
发文量
869
审稿时长
46 days
期刊介绍: Current Biology is a comprehensive journal that showcases original research in various disciplines of biology. It provides a platform for scientists to disseminate their groundbreaking findings and promotes interdisciplinary communication. The journal publishes articles of general interest, encompassing diverse fields of biology. Moreover, it offers accessible editorial pieces that are specifically designed to enlighten non-specialist readers.
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