PD-1和PD-L1在肌肉浸润性膀胱癌分子亚型中的表达:免疫组织化学特征及其与临床病理特征的相关性

IF 2.3 3区 医学 Q2 PATHOLOGY
Farhang Hooshmand, Maral Mokhtari, Shiva Aminnia, Azin Dashtestani, Ali Reza Rezvani, Mohammadhossein Khorraminejad-Shirazi
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引用次数: 0

摘要

肌浸润性膀胱癌(MIBC)是一种预后多变的异质性疾病,需要实用的分类系统。使用免疫组织化学(IHC)标记的分子分型为治疗指导和评估生存提供了经济有效的方法。此外,MIBC分子亚分类为指导免疫检查点抑制剂治疗提供了实用的方法。方法:我们使用免疫组化标记物GATA3、CK5/6和p16对124例MIBC患者进行评估。病例分为腔内型(GATA3+, CK5/6-)、基础型(GATA3-, CK5/6+)或其他型(GATA3-, CK5/6-)。管腔进一步细分为管腔不稳定(LumU; p16+)和管腔乳头状(肿块;p16-)。我们还评估了MIBC分子亚型的临床病理特征。PD-1和PD-L1的表达相对于临床病理特征和MIBC亚型进行评估。结果:本组病例中,LumU占36.2%,肿块占27.6%,基底占24.8%。结论:使用三种免疫结构标记物将mibc分为LumU、肿块、基础亚型和其他亚型。PD-1在腔型中表达较高,而PD-L1在基底型中主要表达升高。这些发现强调了基于ihc的亚型在指导mibc预后和治疗方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

PD-1 and PD-L1 expression in molecular subtypes of muscle-invasive bladder cancer: immunohistochemical characterization and correlation with clinicopathological features.

PD-1 and PD-L1 expression in molecular subtypes of muscle-invasive bladder cancer: immunohistochemical characterization and correlation with clinicopathological features.

PD-1 and PD-L1 expression in molecular subtypes of muscle-invasive bladder cancer: immunohistochemical characterization and correlation with clinicopathological features.

Introduction: Muscle-invasive bladder cancer (MIBC) is a heterogeneous disease with variable outcomes, necessitating practical classification systems. Molecular subtyping using immunohistochemical (IHC) markers offers a cost-effective approach for therapeutic guidance and assessing survival. Moreover, MIBC molecular subclassification provides a practical approach for guiding immune checkpoint inhibitor therapy.

Methods: We evaluated 124 MIBC cases using IHC markers GATA3, CK5/6, and p16. Cases were classified as luminal (GATA3+, CK5/6-), basal (GATA3-, CK5/6+), or other (GATA3-, CK5/6-). Luminal cases were further subdivided into luminal unstable (LumU; p16+) and luminal papillary (LumP; p16-). Clinicopathological characteristics of MIBC molecular subtypes were also assessed. PD-1 and PD-L1 expression were evaluated relative to clinicopathological features and MIBC subtypes.

Results: In our study, 36.2% of the cases were LumU, 27.6% LumP, and 24.8% basal. The basal subtype generally shows a significantly higher tumor stage (p < 0.05). PD-1 was expressed in 70.5% of cases, with the highest expression in LumU (84.21%). PD-1 expression was significantly higher in the luminal compared to the basal subtype (82.1% vs. 53.8%, p < 0.01). PD-L1, expressed in 40% of cases, was significantly elevated in stage III and considerably higher in basal than luminal subtype (57.7% vs. 34.3%, p < 0.05).

Conclusion: MIBCs were practically subclassified into LumU, LumP, basal, and other subtypes using three IHC markers. PD-1 expression was higher in the luminal subtype, while PD-L1 was predominantly elevated in the basal subtype. These findings highlight the potential of IHC-based subtyping to guide prognosis and treatment in MIBCs.

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来源期刊
Diagnostic Pathology
Diagnostic Pathology 医学-病理学
CiteScore
4.60
自引率
0.00%
发文量
93
审稿时长
1 months
期刊介绍: Diagnostic Pathology is an open access, peer-reviewed, online journal that considers research in surgical and clinical pathology, immunology, and biology, with a special focus on cutting-edge approaches in diagnostic pathology and tissue-based therapy. The journal covers all aspects of surgical pathology, including classic diagnostic pathology, prognosis-related diagnosis (tumor stages, prognosis markers, such as MIB-percentage, hormone receptors, etc.), and therapy-related findings. The journal also focuses on the technological aspects of pathology, including molecular biology techniques, morphometry aspects (stereology, DNA analysis, syntactic structure analysis), communication aspects (telecommunication, virtual microscopy, virtual pathology institutions, etc.), and electronic education and quality assurance (for example interactive publication, on-line references with automated updating, etc.).
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