阿魏酸与雷帕霉素联用对人脐静脉内皮细胞抗衰老作用的协同增强。

Q3 Medicine
Han Li, Qiong Han, Yan Liu, Jiaxin Duan, Beiping Su, Zhenlin Tang, Xinhe Huang
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引用次数: 0

摘要

衰老是一个复杂的过程,涉及细胞、遗传、代谢和线粒体的变化。虽然在了解衰老机制和开发抗衰老药物方面取得了重大进展,但单药治疗仍有局限性。本文旨在探讨阿魏酸(FA)和雷帕霉素(Rapamycin)在抗衰老中的协同作用,并阐明其潜在机制,为未来抗衰老治疗提供新的策略。方法:采用细胞计数试剂盒(CCK-8)和衰老相关β- Gal染色法测定人脐静脉内皮细胞(HUVECs) FA和Rapa的安全浓度范围,采用GraphPad Prism 8.0.2计算EC50。我们评估了d -gal诱导的衰老HUVECs对细胞周期阻滞和ROS的影响,并通过Western Blot和RT-qPCR探索了衰老标志物、炎症因子和纤维化基因的协同机制。结果:CCK-8显示,20-160 μM FA和50-200 pM Rapa对HUVECs增殖有促进作用,FA和pM的EC50分别为37.78 μM和48.32 μM。最佳1:2组合比例显示G0/G1细胞减少,ROS减少,NF-κB p65, p53, IL-1β和TNF-α表达降低。它还能抑制纤维化相关基因的转录,下调衰老标志物,维持细胞稳态。讨论:这些结果与先前的研究一致,强调FA的抗氧化特性和Rapa在mTOR抑制中的作用,表明它们的组合同时针对多种衰老途径。与单一药物干预相比,双重方法-减少氧化损伤,同时调节炎症和纤维化-可能提供更好的疗效。结论:总之,这种双靶点策略为开发先进的抗衰老疗法提供了一条有希望的途径,值得在临床前和临床环境中进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synergistic Enhancement of Anti-aging Effects on Human Umbilical Vein Endothelial Cells Treated With the Combination of Ferulic Acid and Rapamycin.

Introduction: Aging is a complex process involving cellular, genetic, metabolic, and mitochondrial changes. While significant progress has been made in understanding aging mechanisms and developing anti-aging drugs, single-drug treatments have limitations. This paper aims to investigate the synergistic effects of Ferulic acid (FA) and Rapamycin (Rapa) on anti-aging and to elucidate their underlying mechanisms, providing novel strategies for future anti-aging therapies.

Methods: The safe concentration ranges of FA and Rapa for Human umbilical vein endothelial cells (HUVECs) were determined via Cell counting kit (CCK-8) and Senescence-associated β- Gal staining, with EC50 calculated by GraphPad Prism 8.0.2. Effects on cell cycle arrest and ROS in D-gal-induced aging HUVECs were assessed, with synergistic mechanisms explored by Western Blot and RT-qPCR for aging markers, inflammatory factors, and fibrosis genes.

Results: CCK-8 showed that 20-160 μM FA and 50-200 pM Rapa enhanced HUVECs proliferation, with EC50 of 37.78 μM for FA and 48.32 pM for Rapa. The optimal 1:2 combination ratio demonstrated reduced G0/G1 cells, decreased ROS, and lowered NF-κB p65, p53, IL-1β, and TNF-α expression. It also inhibited fibrosis-related gene transcription, downregulating aging markers and maintaining cellular homeostasis.

Discussion: These results align with previous studies highlighting FA's antioxidant properties and Rapa's role in mTOR inhibition, suggesting that their combination targets multiple aging pathways simultaneously. The dual approach-reducing oxidative damage while modulating inflammation and fibrosis-may offer superior efficacy compared to single-drug interventions.

Conclusion: In summary, this dual-target strategy presents a promising avenue for developing advanced anti-aging therapies, warranting further investigation in preclinical and clinical settings.

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来源期刊
Current aging science
Current aging science Medicine-Geriatrics and Gerontology
CiteScore
3.90
自引率
0.00%
发文量
40
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