Andrés Pérez-López, Anju Sharma, Sathyavathi Sundararaju, Mohammed Suleiman, Ruwa Mohamed, Diyna Altrmanini, Ibrahim Hassan, Ogra Marufu, Eman Al Maslamani, Clement K M Tsui, Hiam Chemaitelly, Patrick Tang
{"title":"比较产碳青霉烯酶肠杆菌定落在加沙地带受战争影响的儿童和卡塔尔国家参考中心住院儿童：一项观察性队列研究。","authors":"Andrés Pérez-López, Anju Sharma, Sathyavathi Sundararaju, Mohammed Suleiman, Ruwa Mohamed, Diyna Altrmanini, Ibrahim Hassan, Ogra Marufu, Eman Al Maslamani, Clement K M Tsui, Hiam Chemaitelly, Patrick Tang","doi":"10.1016/j.cmi.2025.08.026","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>To compare the rates and molecular characteristics of carbapenemase-producing Enterobacterales (CPE) from rectal screening swabs in war-affected Palestinian children in the Gaza Strip with those of the local pediatric population at Sidra Medicine in Doha.</p><p><strong>Methods: </strong>Whole-genome sequencing was performed on CPE isolated in screening specimens of Gazan children transferred to our institution from Egyptian hospitals between December 2023 and May 2024 (Gaza cohort) and other pediatric patients between January 2021 and May 2024 (Sidra Cohort).</p><p><strong>Results: </strong>The Sidra cohort included 84 CPE isolates from 79 carriers, whereas the Gaza cohort included 53 isolates from 41 carriers. The Gaza cohort showed a 41.9-fold (95% CI, 22.7-81.6) higher crude incidence rate ratio of colonization. In Sidra cohort, 71 carriers (89.9%) had pre-existing medical conditions, compared with 29 (70.7%) Gazan carriers who had no preexisting medical conditions before the armed conflict between Hamas and Israel began in October 2023. Thirty-two (78.0%) Gazan carriers sustained war-related injuries, with 31 (96.7%) of these patients undergoing at least one surgery in Gaza or Egypt. In the Gaza collection, 47 (88.7%) isolates displayed difficult-to-treat resistance phenotype (DTR), whereas 32 (38.1%) Sidra isolates exhibited this phenotype. NDM-like carbapenemases predominated in the Gaza collection (49 isolates; 92.5%), whereas OXA-48-like were most prevalent in the Sidra collection (41 isolates; 48.8%). Klebsiella pneumoniae and Escherichia coli populations in the Gaza collection were dominated by the high-risk clones ST147 (15/19; 78.9%) and ST167 (13/30; 43.3%). Pairwise single-nucleotide polymorphism analysis of these genotypes revealed genetic diversity, suggesting different sources and transmission chains among Gazan carriers.</p><p><strong>Discussion: </strong>Our study revealed a high CPE colonization rate among war-affected Palestinian children and highlights the potential risk posed by conflict-related health emergencies in the silent spread of multidrug-resistant organisms, particularly high-risk global lineages exhibiting DTR, within the healthcare systems of host countries.</p>","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":8.5000,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of carbapenemase-producing Enterobacterales colonizing war-affected children from the Gaza Strip and hospitalized children from a national reference center in Qatar: an observational cohort study.\",\"authors\":\"Andrés Pérez-López, Anju Sharma, Sathyavathi Sundararaju, Mohammed Suleiman, Ruwa Mohamed, Diyna Altrmanini, Ibrahim Hassan, Ogra Marufu, Eman Al Maslamani, Clement K M Tsui, Hiam Chemaitelly, Patrick Tang\",\"doi\":\"10.1016/j.cmi.2025.08.026\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>To compare the rates and molecular characteristics of carbapenemase-producing Enterobacterales (CPE) from rectal screening swabs in war-affected Palestinian children in the Gaza Strip with those of the local pediatric population at Sidra Medicine in Doha.</p><p><strong>Methods: </strong>Whole-genome sequencing was performed on CPE isolated in screening specimens of Gazan children transferred to our institution from Egyptian hospitals between December 2023 and May 2024 (Gaza cohort) and other pediatric patients between January 2021 and May 2024 (Sidra Cohort).</p><p><strong>Results: </strong>The Sidra cohort included 84 CPE isolates from 79 carriers, whereas the Gaza cohort included 53 isolates from 41 carriers. The Gaza cohort showed a 41.9-fold (95% CI, 22.7-81.6) higher crude incidence rate ratio of colonization. In Sidra cohort, 71 carriers (89.9%) had pre-existing medical conditions, compared with 29 (70.7%) Gazan carriers who had no preexisting medical conditions before the armed conflict between Hamas and Israel began in October 2023. Thirty-two (78.0%) Gazan carriers sustained war-related injuries, with 31 (96.7%) of these patients undergoing at least one surgery in Gaza or Egypt. In the Gaza collection, 47 (88.7%) isolates displayed difficult-to-treat resistance phenotype (DTR), whereas 32 (38.1%) Sidra isolates exhibited this phenotype. NDM-like carbapenemases predominated in the Gaza collection (49 isolates; 92.5%), whereas OXA-48-like were most prevalent in the Sidra collection (41 isolates; 48.8%). Klebsiella pneumoniae and Escherichia coli populations in the Gaza collection were dominated by the high-risk clones ST147 (15/19; 78.9%) and ST167 (13/30; 43.3%). Pairwise single-nucleotide polymorphism analysis of these genotypes revealed genetic diversity, suggesting different sources and transmission chains among Gazan carriers.</p><p><strong>Discussion: </strong>Our study revealed a high CPE colonization rate among war-affected Palestinian children and highlights the potential risk posed by conflict-related health emergencies in the silent spread of multidrug-resistant organisms, particularly high-risk global lineages exhibiting DTR, within the healthcare systems of host countries.</p>\",\"PeriodicalId\":10444,\"journal\":{\"name\":\"Clinical Microbiology and Infection\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":8.5000,\"publicationDate\":\"2025-08-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical Microbiology and Infection\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.cmi.2025.08.026\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Microbiology and Infection","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.cmi.2025.08.026","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Comparison of carbapenemase-producing Enterobacterales colonizing war-affected children from the Gaza Strip and hospitalized children from a national reference center in Qatar: an observational cohort study.
Objectives: To compare the rates and molecular characteristics of carbapenemase-producing Enterobacterales (CPE) from rectal screening swabs in war-affected Palestinian children in the Gaza Strip with those of the local pediatric population at Sidra Medicine in Doha.
Methods: Whole-genome sequencing was performed on CPE isolated in screening specimens of Gazan children transferred to our institution from Egyptian hospitals between December 2023 and May 2024 (Gaza cohort) and other pediatric patients between January 2021 and May 2024 (Sidra Cohort).
Results: The Sidra cohort included 84 CPE isolates from 79 carriers, whereas the Gaza cohort included 53 isolates from 41 carriers. The Gaza cohort showed a 41.9-fold (95% CI, 22.7-81.6) higher crude incidence rate ratio of colonization. In Sidra cohort, 71 carriers (89.9%) had pre-existing medical conditions, compared with 29 (70.7%) Gazan carriers who had no preexisting medical conditions before the armed conflict between Hamas and Israel began in October 2023. Thirty-two (78.0%) Gazan carriers sustained war-related injuries, with 31 (96.7%) of these patients undergoing at least one surgery in Gaza or Egypt. In the Gaza collection, 47 (88.7%) isolates displayed difficult-to-treat resistance phenotype (DTR), whereas 32 (38.1%) Sidra isolates exhibited this phenotype. NDM-like carbapenemases predominated in the Gaza collection (49 isolates; 92.5%), whereas OXA-48-like were most prevalent in the Sidra collection (41 isolates; 48.8%). Klebsiella pneumoniae and Escherichia coli populations in the Gaza collection were dominated by the high-risk clones ST147 (15/19; 78.9%) and ST167 (13/30; 43.3%). Pairwise single-nucleotide polymorphism analysis of these genotypes revealed genetic diversity, suggesting different sources and transmission chains among Gazan carriers.
Discussion: Our study revealed a high CPE colonization rate among war-affected Palestinian children and highlights the potential risk posed by conflict-related health emergencies in the silent spread of multidrug-resistant organisms, particularly high-risk global lineages exhibiting DTR, within the healthcare systems of host countries.
期刊介绍：
Clinical Microbiology and Infection (CMI) is a monthly journal published by the European Society of Clinical Microbiology and Infectious Diseases. It focuses on peer-reviewed papers covering basic and applied research in microbiology, infectious diseases, virology, parasitology, immunology, and epidemiology as they relate to therapy and diagnostics.
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