联合暴露于空气污染物对变应性鼻炎小鼠模型变应性炎症的协同加重作用。

IF 3.4 3区 医学 Q1 OTORHINOLARYNGOLOGY
Joo-Hoo Park, Jee Won Moon, Yeong-In Jo, Hwa Eun Yang, Subin Cho, Hyeongguk Son, Hyun-Woo Yang, Il-Ho Park, Dae Jin Song
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引用次数: 0

摘要

目的:变应性鼻炎(Allergic rhinitis, AR)是一种由环境过敏原引起的慢性鼻黏膜炎症。虽然其病理生理学已经得到了很好的研究,但环境恶化因素的影响,特别是污染物暴露的综合影响,还没有完全了解。本研究旨在评估PM2.5、甲醛和锌共同暴露对过敏性炎症小鼠模型的影响,并表征相关的免疫学和组织病理学反应。方法:用卵清蛋白(OVA)致敏雌性BALB/c小鼠,经鼻刺激诱导变应性鼻炎。在第21-24天,小鼠单独或联合暴露于PM2.5、甲醛和锌中。通过行为观察评估过敏症状,通过分析鼻腔和支气管肺泡灌洗液(NALF和BALF)、血清免疫球蛋白水平、鼻腔组织学和细胞因子谱来评估免疫反应。结果:与单一暴露相比,PM2.5、甲醛和锌的联合暴露显著增强了过敏性炎症。PM2.5和锌的共同暴露导致NALF和BALF中总免疫球蛋白E(IgE)水平、嗜酸性粒细胞浸润、鼻摩擦和Th2/Th17细胞因子的协同增加。组织学分析显示,联合暴露后粘膜重塑和杯状细胞增生增加。其他组合,如PM2.5与甲醛的组合,也会诱导附加或适度增强炎症反应。结论:PM2.5、甲醛和锌共同暴露可加重ova诱导的小鼠变应性炎症,其中PM2.5 +锌的协同作用最强。这些发现强调了污染物-污染物相互作用在过敏性气道疾病中的重要性,并强调了进一步研究阐明长期健康影响和人类相关性的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-pollutants in a Murine Model of Allergic Rhinitis.

Objectives: Allergic rhinitis (AR) is a chronic nasal mucosal inflammation triggered by environmental allergens. Although its pathophysiology has been well studied, the effects of environmental aggravating factors-especially combined pollutant exposure-are not fully understood. This study aimed to evaluate the impact of coexposure to PM2.5, formaldehyde, and zinc on allergic inflammation in a murine model of AR and to characterize the associated immunological and histopathological responses.

Methods: Female BALB/c mice were sensitized with ovalbumin (OVA) and challenged intranasally to induce allergic rhinitis. On days 21-24, mice were exposed to PM2.5, formaldehyde, and zinc either individually or in combination with OVA. Allergic symptoms were assessed through behavioral observation, and immune responses were evaluated by analyzing nasal and bronchoalveolar lavage fluids (NALF and BALF), serum immunoglobulin levels, nasal histology, and cytokine profiles.

Results: Combined exposure to PM2.5, formaldehyde, and zinc significantly enhanced allergic inflammation compared to single exposures. Coexposure to PM2.5 and zinc resulted in synergistic increases in total and OVA-specific immunoglobulin E(IgE) levels, eosinophilic infiltration, nasal rubbing, and Th2/Th17 cytokines in NALF and BALF. Histological analysis revealed increased mucosal remodeling and goblet cell hyperplasia following combined exposure. Other combinations, such as PM2.5 with formaldehyde, also induced additive or modestly enhanced inflammatory responses.

Conclusion: Coexposure to PM2.5, formaldehyde, and zinc aggravated allergic inflammation in an OVA-induced murine model, with PM2.5 + zinc showing the strongest synergistic effects. These findings highlight the importance of pollutant-pollutant interactions in allergic airway diseases and underscore the need for further studies to elucidate longterm health impacts and human relevance.

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来源期刊
CiteScore
4.90
自引率
6.70%
发文量
49
审稿时长
6-12 weeks
期刊介绍: Clinical and Experimental Otorhinolaryngology (Clin Exp Otorhinolaryngol, CEO) is an international peer-reviewed journal on recent developments in diagnosis and treatment of otorhinolaryngology-head and neck surgery and dedicated to the advancement of patient care in ear, nose, throat, head, and neck disorders. This journal publishes original articles relating to both clinical and basic researches, reviews, and clinical trials, encompassing the whole topics of otorhinolaryngology-head and neck surgery. CEO was first issued in 2008 and this journal is published in English four times (the last day of February, May, August, and November) per year by the Korean Society of Otorhinolaryngology-Head and Neck Surgery. The Journal aims at publishing evidence-based, scientifically written articles from different disciplines of otorhinolaryngology field. The readership contains clinical/basic research into current practice in otorhinolaryngology, audiology, speech pathology, head and neck oncology, plastic and reconstructive surgery. The readers are otolaryngologists, head and neck surgeons and oncologists, audiologists, and speech pathologists.
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