Normanda Souza-Melo, Giovanna Henriques de Souza, Wanderley de Souza
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Effects of TcFLA-1BP and TcGP72 Deletion on the Infectivity and Survival of Trypanosoma cruzi in Cell Cultures.
Chagas disease, caused by the protozoan Trypanosoma cruzi, is a neglected tropical disease with limited treatment options and no available vaccine. Understanding the role of proteins in the parasite's biological cycle is critical for advancing vaccine development and optimizing therapies. The flagellar attachment zone (FAZ) proteins play a pivotal role in motility, pathogenicity, and cell division in trypanosomatids, but their functions in T. cruzi are not as well-characterized as in Trypanosoma brucei and Leishmania spp. This study investigates the orthologous TcGP72 and TcFLA-1BP proteins in T. cruzi, focusing on their roles in the infective forms of the parasite. Our findings demonstrate that TcFLA-1BP is important for efficient host cell infection in vitro, indicating its critical role in the parasite's infectivity. Conversely, TcGP72 is nonessential for the infection process, but significantly contributes to cytoskeletal remodeling during the parasite's life cycle. These results provide new insights into the distinct functional roles of FAZ proteins in T. cruzi. Furthermore, the study underscores the importance of TcGP72 in maintaining cellular architecture, reinforcing the relevance of FAZ proteins in the parasite's pathogenesis and structural integrity.
期刊介绍:
Each month, the journal publishes easy-to-assimilate, up-to-the minute reports of experimental findings by researchers using a wide range of the latest techniques. Promoting the aims of cell biologists worldwide, papers reporting on structure and function - especially where they relate to the physiology of the whole cell - are strongly encouraged. Molecular biology is welcome, as long as articles report findings that are seen in the wider context of cell biology. In covering all areas of the cell, the journal is both appealing and accessible to a broad audience. Authors whose papers do not appeal to cell biologists in general because their topic is too specialized (e.g. infectious microbes, protozoology) are recommended to send them to more relevant journals. Papers reporting whole animal studies or work more suited to a medical journal, e.g. histopathological studies or clinical immunology, are unlikely to be accepted, unless they are fully focused on some important cellular aspect.
These last remarks extend particularly to papers on cancer. Unless firmly based on some deeper cellular or molecular biological principle, papers that are highly specialized in this field, with limited appeal to cell biologists at large, should be directed towards journals devoted to cancer, there being very many from which to choose.