猪睾丸小管体细胞的血小板衍生生长因子信号传导。

IF 2.9 3区 生物学 Q3 CELL BIOLOGY
Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-08-21 DOI:10.1007/s00441-025-04004-x
Nathalia de Lima E Martins Lara, Maryam Ahmadi Jeyhoonabadi, Rkia Dardari, Anja Elsenhans, Lin Su, Sadman Sakib, Ina Dobrinski
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引用次数: 0

摘要

睾丸中控制体细胞相互作用的机制尚未明确。血小板衍生生长因子(PDGF)途径介导上皮-间质相互作用,并参与啮齿类动物睾丸形态发生。然而,它在高等哺乳动物睾丸中的作用在很大程度上仍然未知。在这里,我们研究了PDGF信号抑制如何影响未成熟(1周龄)猪睾丸小管体细胞(TTSCs),包括细胞间通讯和形态发生。根据scRNA-seq数据,我们在睾丸细胞之间的串扰中建立了PDGF通路特征,确定了支持细胞是PDGF的主要来源,小管周围肌样细胞是PDGF的主要受体。此外,我们发现CP673451抑制PDGF会影响TTSC功能、增殖和细胞骨架,减少细胞面积、局灶黏附大小和纤维连接蛋白的产生,并降低管周肌样细胞特异性基因的表达。PDGF抑制不影响体外睾丸类器官的形成和小管形态发生,但它与小管表面细胞质延伸的消融有关,可能与TTSCs与细胞外基质之间的相互作用有关。PDGF信号可以通过初级纤毛和对环境刺激作出反应的感觉细胞器进行转导,PDGF抑制增加了ttsc中纤毛细胞的百分比和纤毛长度。对猪睾丸初级纤毛的综合形态学分析表明,这些纤毛仍被淹没在细胞质中。综上所述,PDGF信号通路在未成熟猪睾丸中具有活性,并可能通过影响细胞外基质相互作用、细胞骨架和初级纤毛来影响睾丸形态发生。然而,原发性纤毛调节的PDGF信号在睾丸中的作用仍有待确定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Platelet-derived growth factor signaling in porcine testicular tubular somatic cells.

Mechanisms governing somatic cell interactions in the testis are not well defined. The platelet-derived growth factor (PDGF) pathway mediates epithelial-mesenchymal interactions and is involved in testicular morphogenesis in rodents. However, its roles in the testis of higher mammals remain largely unknown. Here, we investigated how PDGF signaling inhibition affects immature (1-week-old) porcine testicular tubular somatic cells (TTSCs), including cell-cell communication and morphogenesis. From scRNA-seq data, we established the PDGF pathway signatures in crosstalk between testicular cells, identifying Sertoli cells as the primary source and peritubular myoid cells as the main recipients of PDGF. Further, we demonstrated that PDGF inhibition by CP673451 affects TTSC functions, proliferation and cytoskeleton, with reduced cell area, focal adhesion size and fibronectin production, and decreased expression of peritubular myoid cell-specific genes. PDGF inhibition did not impair testicular organoid formation and tubule morphogenesis in vitro, but it correlated with ablation of cytoplasmic extensions from the tubule surface, potentially related to interactions between TTSCs and the extracellular matrix. PDGF signaling can be transduced by primary cilia, sensory organelles that respond to environmental stimuli, and PDGF inhibition increased the percentage of ciliated cells and ciliary length in TTSCs. Comprehensive morphological characterization of primary cilia in the porcine testis indicated that these remain submerged in the cytoplasm. In conclusion, the PDGF signaling pathway is active in the immature pig testis and may influence testis morphogenesis by affecting cell-extracellular matrix interaction, cytoskeleton and primary cilia. However, the role of primary cilia-modulated PDGF signaling in the testis remains to be determined.

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来源期刊
Cell and Tissue Research
Cell and Tissue Research 生物-细胞生物学
CiteScore
7.00
自引率
2.80%
发文量
142
审稿时长
1 months
期刊介绍: The journal publishes regular articles and reviews in the areas of molecular, cell, and supracellular biology. In particular, the journal intends to provide a forum for publishing data that analyze the supracellular, integrative actions of gene products and their impact on the formation of tissue structure and function. Submission of papers with an emphasis on structure-function relationships as revealed by recombinant molecular technologies is especially encouraged. Areas of research with a long-standing tradition of publishing in Cell & Tissue Research include: - neurobiology - neuroendocrinology - endocrinology - reproductive biology - skeletal and immune systems - development - stem cells - muscle biology.
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