Wen Peng, Yajing Wang, Manlin Wei, Shiba Liu, Kai Liu, Ming Xiao, Runze Zhang, Yuxiang Wang, Yongjie Zheng, Lue Fang, Yuxi Liu, Chunpeng Zhu, Weijing Meng, Chenyang Dong, Wanxin Ma
{"title":"不同季节放牧和补饲对科尔沁肉牛瘤胃微生物适应性变化对瘤胃发酵和营养物质消化的影响","authors":"Wen Peng, Yajing Wang, Manlin Wei, Shiba Liu, Kai Liu, Ming Xiao, Runze Zhang, Yuxiang Wang, Yongjie Zheng, Lue Fang, Yuxi Liu, Chunpeng Zhu, Weijing Meng, Chenyang Dong, Wanxin Ma","doi":"10.1186/s12866-025-04199-7","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The purpose of this study was to investigate the effects of changes in rumen microbial adaptability on rumen fermentation and nutrient digestion in Horqin beef cattle during different seasons of grazing and supplementary feeding. Four healthy female Horqin beef cattle were selected as experimental animals for grazing and supplementary feeding. The feed, feces, and rumen fluid were collected in spring (March), summer (June), autumn (September), and winter (December) to determine nutrient digestibility, rumen fermentation parameters and microflora. The microflora was sequenced with 16S rRNA.</p><p><strong>Results: </strong>The results revealed that the rumen volatile fatty acid, ammonia nitrogen and microbial protein concentrations in summer were significantly higher than those in the other seasons (P < 0.05). The digestibility of dry matter, organic matter, crude protein and ether extracts decreased significantly in autumn and winter (P < 0.05), especially in winter. Compared with autumn, the rumen bacterial diversity of beef cattle increased significantly in spring (P < 0.05). The results of the rumen microflora revealed that Prevotella was the most dominant bacteria in the rumen of beef cattle in summer, and Rikenellaceae_RC9_gut_group was the most dominant bacteria in the other seasons. The low quality of forage in spring, autumn and winter resulted in an increase in the relative abundance of Rikenellaceae_RC9_gut_group, NK4A214_group, and Christensenellaceae_R-7_group, etc., which are associated with fibre decomposition. In addition, nucleotide metabolism and glycan biosynthesis and metabolism increased significantly in bacteria in the summer (P < 0.05).</p><p><strong>Conclusions: </strong>The results revealed the dynamic adaptability of the rumen microbiota to seasonal and dietary changes and provide references for the grazing and supplementary feeding of Horqin beef cattle in different seasons.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"556"},"PeriodicalIF":4.2000,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12382152/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effects of changes in rumen microbial adaptability on rumen fermentation and nutrient digestion in Horqin beef cattle during different seasons of grazing and supplementary feeding.\",\"authors\":\"Wen Peng, Yajing Wang, Manlin Wei, Shiba Liu, Kai Liu, Ming Xiao, Runze Zhang, Yuxiang Wang, Yongjie Zheng, Lue Fang, Yuxi Liu, Chunpeng Zhu, Weijing Meng, Chenyang Dong, Wanxin Ma\",\"doi\":\"10.1186/s12866-025-04199-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The purpose of this study was to investigate the effects of changes in rumen microbial adaptability on rumen fermentation and nutrient digestion in Horqin beef cattle during different seasons of grazing and supplementary feeding. Four healthy female Horqin beef cattle were selected as experimental animals for grazing and supplementary feeding. The feed, feces, and rumen fluid were collected in spring (March), summer (June), autumn (September), and winter (December) to determine nutrient digestibility, rumen fermentation parameters and microflora. The microflora was sequenced with 16S rRNA.</p><p><strong>Results: </strong>The results revealed that the rumen volatile fatty acid, ammonia nitrogen and microbial protein concentrations in summer were significantly higher than those in the other seasons (P < 0.05). The digestibility of dry matter, organic matter, crude protein and ether extracts decreased significantly in autumn and winter (P < 0.05), especially in winter. Compared with autumn, the rumen bacterial diversity of beef cattle increased significantly in spring (P < 0.05). The results of the rumen microflora revealed that Prevotella was the most dominant bacteria in the rumen of beef cattle in summer, and Rikenellaceae_RC9_gut_group was the most dominant bacteria in the other seasons. The low quality of forage in spring, autumn and winter resulted in an increase in the relative abundance of Rikenellaceae_RC9_gut_group, NK4A214_group, and Christensenellaceae_R-7_group, etc., which are associated with fibre decomposition. In addition, nucleotide metabolism and glycan biosynthesis and metabolism increased significantly in bacteria in the summer (P < 0.05).</p><p><strong>Conclusions: </strong>The results revealed the dynamic adaptability of the rumen microbiota to seasonal and dietary changes and provide references for the grazing and supplementary feeding of Horqin beef cattle in different seasons.</p>\",\"PeriodicalId\":9233,\"journal\":{\"name\":\"BMC Microbiology\",\"volume\":\"25 1\",\"pages\":\"556\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2025-08-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12382152/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1186/s12866-025-04199-7\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s12866-025-04199-7","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Effects of changes in rumen microbial adaptability on rumen fermentation and nutrient digestion in Horqin beef cattle during different seasons of grazing and supplementary feeding.
Background: The purpose of this study was to investigate the effects of changes in rumen microbial adaptability on rumen fermentation and nutrient digestion in Horqin beef cattle during different seasons of grazing and supplementary feeding. Four healthy female Horqin beef cattle were selected as experimental animals for grazing and supplementary feeding. The feed, feces, and rumen fluid were collected in spring (March), summer (June), autumn (September), and winter (December) to determine nutrient digestibility, rumen fermentation parameters and microflora. The microflora was sequenced with 16S rRNA.
Results: The results revealed that the rumen volatile fatty acid, ammonia nitrogen and microbial protein concentrations in summer were significantly higher than those in the other seasons (P < 0.05). The digestibility of dry matter, organic matter, crude protein and ether extracts decreased significantly in autumn and winter (P < 0.05), especially in winter. Compared with autumn, the rumen bacterial diversity of beef cattle increased significantly in spring (P < 0.05). The results of the rumen microflora revealed that Prevotella was the most dominant bacteria in the rumen of beef cattle in summer, and Rikenellaceae_RC9_gut_group was the most dominant bacteria in the other seasons. The low quality of forage in spring, autumn and winter resulted in an increase in the relative abundance of Rikenellaceae_RC9_gut_group, NK4A214_group, and Christensenellaceae_R-7_group, etc., which are associated with fibre decomposition. In addition, nucleotide metabolism and glycan biosynthesis and metabolism increased significantly in bacteria in the summer (P < 0.05).
Conclusions: The results revealed the dynamic adaptability of the rumen microbiota to seasonal and dietary changes and provide references for the grazing and supplementary feeding of Horqin beef cattle in different seasons.
期刊介绍：
BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
