金木豆早熟基因全基因组关联图谱研究。

IF 2.2 4区 农林科学 Q2 AGRONOMY
Breeding Science Pub Date : 2025-04-01 Epub Date: 2025-03-27 DOI:10.1270/jsbbs.24054
Naoya Yamaguchi, Keisuke Tanaka, Kosuke Nakagawa, Hirokazu Sato, Akito Hosoi, Yozo Nakazawa
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引用次数: 0

摘要

日本红豆或白豆(Phaseolus vulgaris L.)品种,用于制作甜煮豆,被称为“kintoki”豆。在日本北部的北海道,在冬小麦轮作之前种植金木豆。因此,早熟对他们来说是一个重要的特征。本研究的目的是通过全基因组关联研究(GWAS)绘制与金木豆日数到成熟度相关的基因组区域。GWAS在3 年的1号染色体(Pv01)上检测到与成熟日数相关的显著单核苷酸多态性,并将早熟候选区域定位在473 kb的区域。测序分析表明,光敏色素A3基因phvull . 001g221100可能与kintoki品种早熟有关:Pv01上外显子47 644 850位置的胞嘧啶插入导致移码产生提前终止密码子。我们的研究结果表明,phvull . 001g221100的功能缺失突变来源于主要品种“Taisho-Kintoki”,并起源于最古老的kintoki品种“Hon-Kintoki”的自发突变。以光敏色素A3功能插入为目标的DNA标记将为金豆育种中的标记辅助选择提供依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Genome-wide association mapping for early maturity in kintoki bean (<i>Phaseolus vulgaris</i> L.).

Genome-wide association mapping for early maturity in kintoki bean (<i>Phaseolus vulgaris</i> L.).

Genome-wide association mapping for early maturity in kintoki bean (<i>Phaseolus vulgaris</i> L.).

Genome-wide association mapping for early maturity in kintoki bean (Phaseolus vulgaris L.).

Japanese red or white common bean (Phaseolus vulgaris L.) cultivars, used to make sweetened boiled beans, are called "kintoki" beans. Kintoki beans are planted to precede winter wheat for crop rotation in Hokkaido, northern Japan. Therefore, early maturity is an important trait for them. The aim of this study was to map the genomic region associated with days to maturity in kintoki beans by genome-wide association study (GWAS). Significant single nucleotide polymorphisms associated with days to maturity were detected on chromosome 1 (Pv01) by GWAS in 3 years, and the candidate region for early maturity was mapped to a 473-kb region. Sequencing analysis indicated that Phvul.001G221100, a phytochrome A3 gene, is likely to be responsible for early maturity in kintoki cultivars: the insertion of a cytosine in exon 1 at position 47 644 850 on Pv01 causes a frameshift that creates an early stop codon. Our findings suggest that the loss-of-function mutation of Phvul.001G221100 is derived from a leading cultivar, 'Taisho-Kintoki', and is originated from a spontaneous mutation in the oldest kintoki cultivar, 'Hon-Kintoki'. The DNA markers targeting the functional insertion of phytochrome A3 will be useful for marker-assisted selection in kintoki bean breeding.

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来源期刊
Breeding Science
Breeding Science 农林科学-农艺学
CiteScore
4.90
自引率
4.20%
发文量
37
审稿时长
1.5 months
期刊介绍: Breeding Science is published by the Japanese Society of Breeding. Breeding Science publishes research papers, notes and reviews related to breeding. Research Papers are standard original articles. Notes report new cultivars, breeding lines, germplasms, genetic stocks, mapping populations, database, software, and techniques significant and useful for breeding. Reviews summarize recent and historical events related breeding. Manuscripts should be submitted by corresponding author. Corresponding author must have obtained permission from all authors prior to submission. Correspondence, proofs, and charges of excess page and color figures should be handled by the corresponding author.
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