CgDbf2/CgMob1-CgCdc14磷酸化模块介导的磷酸酶活性调控炭疽菌MEN信号传导和致病性

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Jiyun Yang, Mei-Ling Sun, Yu-Ting Pan, Chuan-Xu Peng, Si-Qian Yuan, Ye-Dan Li, Jia-Hong Ren, Lin Huang
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引用次数: 0

摘要

有丝分裂退出网络(MEN)在真核生物细胞形态和致病性调控中起着关键作用。然而,在植物致病真菌中,MEN信号通路如何整合和协调形态学、宿主源性ROS稳态和毒力的确切机制仍然知之甚少。本研究结合RNA-seq、生化和遗传分析,发现CgDbf2和CgMob1在炭黑病菌gloeosporioides中形成蛋白复合物。作为MEN信号通路的重要组成部分,CgDbf2/CgMob1复合物对于维持细胞壁完整性(CWI)和对杀菌剂多菌灵的抗性至关重要。CgDBF2和CgMOB1的破坏导致毒力显著降低,主要是由于附着胞形成受损和侵袭性生长能力减弱。此外,我们的研究结果表明CgDbf2与磷酸酶CgCdc14相互作用并使其磷酸化。CgCdc14的Ser427和Thr428残基是CgDbf2磷酸化的关键位点,可增强CgCdc14的磷酸酶活性,对真菌的毒力起作用。此外,我们的研究结果强调,Dbf2/Mob1-Cdc14轴协调CWI和对活性氧的响应,这两者都是植物感染的关键。我们的数据强调了CgCdc14的磷酸酶活性,它依赖于CgDbf2的磷酸化,对于MEN和CWI之间的串扰至关重要。这些发现为植物病原真菌侵染植物的分子机制提供了新的见解,并为开发抗真菌策略提供了潜在的靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The CgDbf2/CgMob1-CgCdc14 Phosphorylation Module Mediated Phosphatase Activity Regulates MEN Signalling and Pathogenicity in Colletotrichum gloeosporioides.

The mitotic exit network (MEN) plays pivotal roles in regulating cell morphology and pathogenicity in eukaryotic organisms. However, the precise mechanisms underlying how the MEN signalling pathway integrates and coordinates morphologiensis, host derived ROS homoeostasis and virulence remains poorly understood in phytopathogenic fungi. In this study, combined with RNA-seq, biochemical and genetic analyses, we found that CgDbf2 and CgMob1 form a protein complex in Colletotrichum gloeosporioides. As an essential component of MEN signalling pathway, CgDbf2/CgMob1 complex is critical for maintaining cell wall integrity (CWI), and enabling resistance to the fungicide carbendazim. Disruption of CgDBF2 and CgMOB1 results in significantly reduced virulence, primarily due to impaired appressorium formation and diminished invasive growth capabilities. Additionally, our findings reveal that CgDbf2 interacts with and phosphorylates the phosphatase CgCdc14. The residues Ser427 and Thr428 of CgCdc14 are critical sites phosphorylated by CgDbf2, enhancing CgCdc14 phosphatase activity and contributing to fungal virulence. Moreover, our findings highlight that the Dbf2/Mob1-Cdc14 axis coordinates CWI and responses to reactive oxygen species, both of which are crucial for plant infection. Our data underscore that the phosphatase activity of CgCdc14, which is dependent on phosphorylation by CgDbf2, is essential for crosstalk between MEN and CWI. These findings offer novel insights into the molecular mechanisms governing plant infection of phytopathogenic fungi and provide potential targets for developing antifungal strategies.

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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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