鉴别2-（二甲氧基）- n -（2-（三氟甲氧基）苄基）乙胺系列的位置异构体：色谱-质谱法。

IF 2.7 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Drug Testing and Analysis Pub Date : 2025-07-30 DOI:10.1002/dta.3936

Olga V Kupriyanova, Vadim A Shevyrin, Raziya G Sadykova, Yuri M Shafran

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引用次数: 0

摘要

苯基乙胺的合成衍生物，特别是2-（2,5-二甲氧基苯基）- n -（（2-取代）苄基）乙胺，经常出现在全球新设计药物市场上，据报道与食用这些药物的人的不良反应有关。同时，它们的位置异构体可能缺乏精神作用。这些化合物目前不受立法管制，将来可能用于医疗目的。在继续研究具有不同取代基的2-（2,5-二甲氧基苯基）- n -（2-取代）苄基)乙胺的性质的同时，对n -苄基片段在邻位上具有三氟甲氧基（OCF3）的2-（二甲氧基苯基）- n -(2-（三氟甲氧基）苄基乙胺（NBOMe(F)）的研究很少。使用HP-5型色谱柱时，气相色谱分析显示存在一对关键的异构体，在柱恒温器的不同温度条件下难以区分。在210℃的等温条件下，在DB-17MS色谱柱上进行分离。本文还提出了用薄层色谱法区分这对关键异构体的方法。测定了NBOMe(F)系列同分异构体及其n -三氟乙酰基衍生物的保留指数，可作为气相色谱分析的附加鉴别标准。研究表明，只有记录了NBOMe(F)异构体本身及其n -三氟乙酰基衍生物的谱，才有可能用电子电离质谱来区分NBOMe(F)异构体。作为鉴别异构体化合物的一种替代方法，提出了一种不需要衍生化的高效液相色谱-串联质谱方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Differentiating Positional Isomers of the 2-(Dimethoxyphenyl)-N-(2-(trifluoromethoxy)benzyl)ethanamine Series: A Chromatography-Mass Spectrometry Approach.

Synthetic derivatives of phenylethanamine, specifically 2-(2,5-dimethoxyphenyl)-N-((2-substituted)benzyl)ethanamines, regularly appear on the global market of new designer drugs and have been reported to be associated with adverse effects in humans who consume them. At the same time, their positional isomers may lack psychoactive effects. These compounds are not currently regulated by legislation and could potentially be used for medical purposes in the future. Continuing the study of the properties of 2-(2,5-dimethoxyphenyl)-N-((2-substituted)benzyl)ethanamines with different substituents, a poorly studied series of 2-(dimethoxyphenyl)-N-(2-(trifluoromethoxy)benzyl)ethanamines (NBOMe(F)) with a trifluoromethoxy group (OCF₃) in the ortho-position of the N-benzyl fragment is of interest. Gas chromatographic analysis revealed the existence of a critical pair of isomers when using an HP-5 type column, the differentiation of which is difficult under varying temperature conditions of the column thermostat. Their separation was achieved on a DB-17MS column under isothermal conditions at a temperature of 210°C. A method using thin-layer chromatography for the differentiation of this critical pair of isomers is also proposed. Retention indices of the isomeric compounds of the NBOMe(F) series and their N-trifluoroacetyl derivatives have been determined, which can serve as an additional identification criterion for gas chromatographic analysis. It has been shown that differentiation of NBOMe(F) isomers using electron ionization mass spectra is only possible if the spectra of both the isomers themselves and their N-trifluoroacetyl derivatives, which exhibit significant differences, are recorded. As an alternative method for differentiating the isomeric compounds, an approach using high-performance liquid chromatography-tandem mass spectrometry, which does not require derivatization, is proposed.

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来源期刊

Drug Testing and Analysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

5.90

自引率

24.10%

发文量

191

审稿时长

2.3 months

期刊介绍： As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances. In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds). Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.