一种基于芒果ii的遗传编码系统，具有更高的亮度和选择性，用于成像活细胞中的rna。

IF 2.7 3区化学 Q1 CHEMISTRY, ORGANIC

Organic & Biomolecular Chemistry Pub Date : 2025-07-10 DOI:10.1039/d5ob01004g

Julia I. Svetlova , Oksana S. Bychenko , Georgy K. Slushko , Yulia V. Skvortsova , Evgeny S. Belyaev , Polina N. Kamzeeva , Yuriy M. Khodarovich , Alexey A. Pakhomov , Tatyana L. Azhikina , Anna M. Varizhuk , Andrey V. Aralov

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引用次数: 0

摘要

很少有基于适体-染料对的遗传编码系统在亮度、对比度等方面满足生物成像的所有要求，因此需要对其进行优化。在这里，我们报道了一种新的无生物素荧光配体，与已知染料相比，它在试管中比其他DNA/RNA结构和活细胞中遗传编码的适体标记RNA复合物中具有更高的选择性和亮度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A genetically encoded Mango II-based system with increased brightness and selectivity for imaging RNAs in live cells

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A genetically encoded Mango II-based system with increased brightness and selectivity for imaging RNAs in live cells

Few genetically encoded systems based on aptamer–dye pairs meet all the requirements of bioimaging in terms of brightness, contrast, etc., thus necessitating their optimization. Here, we report a new biotin-free fluorogenic ligand that demonstrates increased selectivity for Mango II over other DNA/RNA structures in tube and brightness in complex with genetically encoded aptamer-labeled RNA in live cells compared to the known dye.

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来源期刊

Organic & Biomolecular Chemistry 化学-有机化学

CiteScore

5.50

自引率

9.40%

发文量

1056

审稿时长

1.3 months

期刊介绍： Organic & Biomolecular Chemistry is an international journal using integrated research in chemistry-organic chemistry. Founded in 2003 by the Royal Society of Chemistry, the journal is published in Semimonthly issues and has been indexed by SCIE, a leading international database. The journal focuses on the key research and cutting-edge progress in the field of chemistry-organic chemistry, publishes and reports the research results in this field in a timely manner, and is committed to becoming a window and platform for rapid academic exchanges among peers in this field. The journal's impact factor in 2023 is 2.9, and its CiteScore is 5.5.