{"title":"低强度运动诱导的miR-486a-3p分泌对增强小鼠共情行为的潜在贡献:可能涉及棕色脂肪组织","authors":"Takeru Shima, Keisuke Yoshii, Yuika Yoshikawa, Chiho Terashima","doi":"10.1016/j.brainres.2025.149923","DOIUrl":null,"url":null,"abstract":"<div><div>Empathy plays a crucial role in the maintenance of interpersonal relationships among mammals. Remarkably, engaging in light-intensity exercise has been identified as a facilitator of empathic behavior, a phenomenon associated with the upregulation of miR-486a-3p in the insular cortex. However, it remains to cover the contribution of miR-486a-3p and the mechanisms of changing levels of that in the insular cortex with light-intensity exercise. We initially assessed the impact of light-intensity exercise (7.0 m/min, 30 min/day, five days/week for four weeks) on helping behavior, mRNA in their insular cortex, and the secretion of exosomal miR-486a-3p from their peripheral tissues. Subsequently, we explored the effects of a daily intraperitoneal injection of miR-486a-3p mimic over a two-week period on helping behavior. The intervention of light-intensity exercise, which enhanced helping behavior, resulted in elevated levels of miR-486a-3p in the insular cortex and exosomal miR-486a-3p in the plasma. Interestingly, the exercise intervention led to a marked increase in exosomal miR-486a-3p derived from brown adipose tissue, whereas no such increase was observed in exosomes originating from the gastrocnemius muscle or the liver. Moreover, the administration of mmu-miR-486a-3p mimic exhibited a similar enhancement of helping behavior in mice. Notably, both the exercise intervention and miR-486a-3p mimic treatment led to the downregulation of <em>Pten</em> mRNA and upregulation of <em>Bdnf</em> mRNA in the insular cortex. Our findings suggest that the increase in peripheral-derived miR-486a-3p, originating from the brown adipose tissue, may support empathy enhancement associated with light-intensity exercise. Additionally, miR-486a-3p may have similar effects to light-intensity exercise in relation to empathy, providing a potential approach for addressing empathy-related behaviors.</div></div>","PeriodicalId":9083,"journal":{"name":"Brain Research","volume":"1866 ","pages":"Article 149923"},"PeriodicalIF":2.6000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The potential contribution of light-intensity exercise-induced miR-486a-3p secretion on enhancing empathic behavior in mice: Possible involvement of brown adipose tissue\",\"authors\":\"Takeru Shima, Keisuke Yoshii, Yuika Yoshikawa, Chiho Terashima\",\"doi\":\"10.1016/j.brainres.2025.149923\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Empathy plays a crucial role in the maintenance of interpersonal relationships among mammals. Remarkably, engaging in light-intensity exercise has been identified as a facilitator of empathic behavior, a phenomenon associated with the upregulation of miR-486a-3p in the insular cortex. However, it remains to cover the contribution of miR-486a-3p and the mechanisms of changing levels of that in the insular cortex with light-intensity exercise. We initially assessed the impact of light-intensity exercise (7.0 m/min, 30 min/day, five days/week for four weeks) on helping behavior, mRNA in their insular cortex, and the secretion of exosomal miR-486a-3p from their peripheral tissues. Subsequently, we explored the effects of a daily intraperitoneal injection of miR-486a-3p mimic over a two-week period on helping behavior. The intervention of light-intensity exercise, which enhanced helping behavior, resulted in elevated levels of miR-486a-3p in the insular cortex and exosomal miR-486a-3p in the plasma. Interestingly, the exercise intervention led to a marked increase in exosomal miR-486a-3p derived from brown adipose tissue, whereas no such increase was observed in exosomes originating from the gastrocnemius muscle or the liver. Moreover, the administration of mmu-miR-486a-3p mimic exhibited a similar enhancement of helping behavior in mice. Notably, both the exercise intervention and miR-486a-3p mimic treatment led to the downregulation of <em>Pten</em> mRNA and upregulation of <em>Bdnf</em> mRNA in the insular cortex. Our findings suggest that the increase in peripheral-derived miR-486a-3p, originating from the brown adipose tissue, may support empathy enhancement associated with light-intensity exercise. Additionally, miR-486a-3p may have similar effects to light-intensity exercise in relation to empathy, providing a potential approach for addressing empathy-related behaviors.</div></div>\",\"PeriodicalId\":9083,\"journal\":{\"name\":\"Brain Research\",\"volume\":\"1866 \",\"pages\":\"Article 149923\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brain Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S000689932500486X\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brain Research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S000689932500486X","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
The potential contribution of light-intensity exercise-induced miR-486a-3p secretion on enhancing empathic behavior in mice: Possible involvement of brown adipose tissue
Empathy plays a crucial role in the maintenance of interpersonal relationships among mammals. Remarkably, engaging in light-intensity exercise has been identified as a facilitator of empathic behavior, a phenomenon associated with the upregulation of miR-486a-3p in the insular cortex. However, it remains to cover the contribution of miR-486a-3p and the mechanisms of changing levels of that in the insular cortex with light-intensity exercise. We initially assessed the impact of light-intensity exercise (7.0 m/min, 30 min/day, five days/week for four weeks) on helping behavior, mRNA in their insular cortex, and the secretion of exosomal miR-486a-3p from their peripheral tissues. Subsequently, we explored the effects of a daily intraperitoneal injection of miR-486a-3p mimic over a two-week period on helping behavior. The intervention of light-intensity exercise, which enhanced helping behavior, resulted in elevated levels of miR-486a-3p in the insular cortex and exosomal miR-486a-3p in the plasma. Interestingly, the exercise intervention led to a marked increase in exosomal miR-486a-3p derived from brown adipose tissue, whereas no such increase was observed in exosomes originating from the gastrocnemius muscle or the liver. Moreover, the administration of mmu-miR-486a-3p mimic exhibited a similar enhancement of helping behavior in mice. Notably, both the exercise intervention and miR-486a-3p mimic treatment led to the downregulation of Pten mRNA and upregulation of Bdnf mRNA in the insular cortex. Our findings suggest that the increase in peripheral-derived miR-486a-3p, originating from the brown adipose tissue, may support empathy enhancement associated with light-intensity exercise. Additionally, miR-486a-3p may have similar effects to light-intensity exercise in relation to empathy, providing a potential approach for addressing empathy-related behaviors.
期刊介绍:
An international multidisciplinary journal devoted to fundamental research in the brain sciences.
Brain Research publishes papers reporting interdisciplinary investigations of nervous system structure and function that are of general interest to the international community of neuroscientists. As is evident from the journals name, its scope is broad, ranging from cellular and molecular studies through systems neuroscience, cognition and disease. Invited reviews are also published; suggestions for and inquiries about potential reviews are welcomed.
With the appearance of the final issue of the 2011 subscription, Vol. 67/1-2 (24 June 2011), Brain Research Reviews has ceased publication as a distinct journal separate from Brain Research. Review articles accepted for Brain Research are now published in that journal.