{"title":"长期使用益生菌治疗可减轻衰老加速小鼠的肠道功能障碍和肠道阿尔茨海默病相关病理","authors":"Vanessa D'Antongiovanni , Clarissa Pierucci , Laura Benvenuti , Annalisa Visciglia , Cristina Segnani , Chiara Ippolito , Luca Antonioli , Matteo Fornai , Giovanni Deusebio , Marco Pane , Angela Amoruso , Nunzia Bernardini , Carolina Pellegrini","doi":"10.1016/j.jff.2025.107016","DOIUrl":null,"url":null,"abstract":"<div><div>The manipulation of gut microbiota represents a potential therapeutic strategy to counteract gut disturbances in patients with age-related cognitive disorders. Here, we investigated the effects of a novel probiotic mixture (PM) in counteracting intestinal symptoms and enteric AD-pathology in an accelerated-senescence mouse model. SAMP8 mice were treated orally with PM at 1 <sub>×</sub> 10<sup>9</sup> CFU\\AFU strain/mouse/day or placebo for two months. The effects of PM on <em>in vivo</em> colonic transit and <em>in vitro</em> colonic contractile activity were evaluated. Changes in cholinergic, tachykininergic and nitrergic neuron density as well as colonic amyloid-β1–42 (Aβ), HuC/D and IL-1β levels were assessed. Plasma GABA concentrations were also investigated. SAMP8 mice displayed: 1) impairments in colonic transit and <em>in vitro</em> contractility; 2) decrease in myenteric cholinergic, tachykininergic and nitrergic neurons; 3) increase in colonic Aβ1–42 and IL-1β levels and 4) decrease in circulating GABA concentrations. PM restored <em>in vivo</em> and <em>in vitro</em> colonic motility by normalizing enteric cholinergic, tachykininergic and nitrergic neurotransmissions and decreased enteric AD-related protein accumulation and inflammation. Finally, PM induced a modulation of plasma GABA concentrations in SAMP8 mice. Overall, PM supplementation may represent a suitable therapeutic strategy to counteract gut disturbances related to age-related cognitive disorders.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"133 ","pages":"Article 107016"},"PeriodicalIF":4.0000,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prolonged treatment with probiotics mitigates intestinal dysfunction and enteric Alzheimer's-related pathology in senescence-accelerated mice\",\"authors\":\"Vanessa D'Antongiovanni , Clarissa Pierucci , Laura Benvenuti , Annalisa Visciglia , Cristina Segnani , Chiara Ippolito , Luca Antonioli , Matteo Fornai , Giovanni Deusebio , Marco Pane , Angela Amoruso , Nunzia Bernardini , Carolina Pellegrini\",\"doi\":\"10.1016/j.jff.2025.107016\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The manipulation of gut microbiota represents a potential therapeutic strategy to counteract gut disturbances in patients with age-related cognitive disorders. Here, we investigated the effects of a novel probiotic mixture (PM) in counteracting intestinal symptoms and enteric AD-pathology in an accelerated-senescence mouse model. SAMP8 mice were treated orally with PM at 1 <sub>×</sub> 10<sup>9</sup> CFU\\\\AFU strain/mouse/day or placebo for two months. The effects of PM on <em>in vivo</em> colonic transit and <em>in vitro</em> colonic contractile activity were evaluated. Changes in cholinergic, tachykininergic and nitrergic neuron density as well as colonic amyloid-β1–42 (Aβ), HuC/D and IL-1β levels were assessed. Plasma GABA concentrations were also investigated. SAMP8 mice displayed: 1) impairments in colonic transit and <em>in vitro</em> contractility; 2) decrease in myenteric cholinergic, tachykininergic and nitrergic neurons; 3) increase in colonic Aβ1–42 and IL-1β levels and 4) decrease in circulating GABA concentrations. PM restored <em>in vivo</em> and <em>in vitro</em> colonic motility by normalizing enteric cholinergic, tachykininergic and nitrergic neurotransmissions and decreased enteric AD-related protein accumulation and inflammation. Finally, PM induced a modulation of plasma GABA concentrations in SAMP8 mice. Overall, PM supplementation may represent a suitable therapeutic strategy to counteract gut disturbances related to age-related cognitive disorders.</div></div>\",\"PeriodicalId\":360,\"journal\":{\"name\":\"Journal of Functional Foods\",\"volume\":\"133 \",\"pages\":\"Article 107016\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2025-09-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Functional Foods\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1756464625003585\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Functional Foods","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1756464625003585","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Prolonged treatment with probiotics mitigates intestinal dysfunction and enteric Alzheimer's-related pathology in senescence-accelerated mice
The manipulation of gut microbiota represents a potential therapeutic strategy to counteract gut disturbances in patients with age-related cognitive disorders. Here, we investigated the effects of a novel probiotic mixture (PM) in counteracting intestinal symptoms and enteric AD-pathology in an accelerated-senescence mouse model. SAMP8 mice were treated orally with PM at 1 × 109 CFU\AFU strain/mouse/day or placebo for two months. The effects of PM on in vivo colonic transit and in vitro colonic contractile activity were evaluated. Changes in cholinergic, tachykininergic and nitrergic neuron density as well as colonic amyloid-β1–42 (Aβ), HuC/D and IL-1β levels were assessed. Plasma GABA concentrations were also investigated. SAMP8 mice displayed: 1) impairments in colonic transit and in vitro contractility; 2) decrease in myenteric cholinergic, tachykininergic and nitrergic neurons; 3) increase in colonic Aβ1–42 and IL-1β levels and 4) decrease in circulating GABA concentrations. PM restored in vivo and in vitro colonic motility by normalizing enteric cholinergic, tachykininergic and nitrergic neurotransmissions and decreased enteric AD-related protein accumulation and inflammation. Finally, PM induced a modulation of plasma GABA concentrations in SAMP8 mice. Overall, PM supplementation may represent a suitable therapeutic strategy to counteract gut disturbances related to age-related cognitive disorders.
期刊介绍:
Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies.
The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients.
The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.