基于成分数据矩阵和逐步匹配策略结合UHPLC-QTOF-MS鉴别胆粉

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-09-01 DOI:10.1016/j.bbrep.2025.102234

Haonan Wu , Xianrui Wang , Minghua Li , Xiaohan Guo , Xianlong Cheng , Tian Yin , Wenguang Jing , Feng Wei

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引用次数: 0

摘要

背景猪胆汁粉（PBP）、鸡胆汁粉（CKBP）、山羊胆汁粉（GBP）、熊胆汁粉（BBP）、牛胆汁粉（CBP）、兔胆汁粉（RBP）和蛇胆汁粉（SBP）是临床上常用的胆汁粉药材。由于混淆和欺诈使用频繁，有必要建立和丰富识别方法。本文创新性地提出了包含共同成分矩阵（CCM）和特异性成分矩阵（SCM）的成分数据矩阵（CDM）和渐进式匹配策略（PMS）来识别PBP、CKBP、GBP、BBP、CBP、RBP和SBP。方法采用LC-MS对样品进行分析，获取化学成分的质谱数据。然后，通过MS数据的相交和去相交得到各胆粉的CCM和SCM。最后，利用CCM和SCM对测试样本进行匹配，得到匹配值（MV）。同时，对专利组件进行了进一步的探索。结果基于CDM和PMS，可快速实现PBP、CKBP、GBP、BBP、CBP、RBP和SBP的鉴别，且MV≥70%。结论：CDM和PMS能有效鉴别PBP、CKBP、GBP、BBP、CBP、RBP和SBP，特异性更强。丰富了鉴定方法，加强了胆粉药材的质量保证。

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Identification of bile powder based on component data matrix and progressive matching strategy combined with UHPLC-QTOF-MS

Background

Pig bile powder (PBP), chicken bile powder (CKBP), goat bile powder (GBP), bear bile powder (BBP), cow bile powder (CBP), rabbit bile powder (RBP) and snake bile powder (SBP) are commonly used bile powder medicinal materials in the clinic. Due to frequent confusion and fraudulent use, it is necessary to establish and enrich identification methods. In this paper, the component data matrix (CDM) contained common component matrix (CCM) and specificity component matrix (SCM), and progressive matching strategy (PMS) were innovatively proposed to identify PBP, CKBP, GBP, BBP, CBP, RBP and SBP.

Methods

The samples were analyzed by LC-MS to obtain MS data on chemical components. Then, the CCM and SCM of each bile powder was obtained by taking the intersection and de-intersection of MS data. Finally, the CCM and SCM were used for matching test samples to obtain matching value (MV). At the same time, the proprietary components were further explored.

Results

Based on CDM and PMS, the identification of PBP, CKBP, GBP, BBP, CBP, RBP and SBP can be quickly realized with MV ≥ 70 %. Conclusions: The CDM and PMS were efficient to identify PBP, CKBP, GBP, BBP, CBP, RBP and SBP, which has more specificity. It can enrich identification methods and strengthen quality assurance of bile powder medicinal materials.

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.