Zhen-Yu Qiang , Dong-Xing Guan , Jia-Lu Gao , Gang Li , Daniel Menezes-Blackburn , Anna Gunina , Yvan Capowiez , Lena Q. Ma
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Endogeic earthworm species <em>Metaphire guillelmi</em> was incubated for 30 days in pots filled with soils Fluvisol or Acrisol with different total P contents (0.6 and 1.2 mg kg<sup>−1</sup>, respectively). Chemical analyses revealed that total P content in earthworm casts increased by 120 % in Fluvisol and 7.7 % in Acrisol. Available P content increased by 8.5 times in Fluvisol and 4.4 times in Acrisol in the drilosphere compared to bulk soil due to elevated acid/alkaline phosphatase activities and intensified Fe/Al-bound P desorption amongst others. Imaging identified co-existing and distinct hotspots for available P and acid phosphatase activity in soils surrounding the burrow walls, with hotspot proportions of 0.1 %‒3.1 % and 5.4 %‒7.5 % of the imaged areas. Earthworm activity increased the abundance of specific bacterial (<em>Aeromonas</em> and <em>Flavobacterium</em>) and fungal (<em>Scedosporium</em> and <em>Podospora</em>) taxa potentially contributing to P mobilization. The correlation between the distribution of metal-bound adsorption sites, phosphatase activity, and diffusion mechanisms contributed to the available P redistribution in the soil. These findings provide insights for developing soil management strategies that harness earthworm-mediated P cycling to optimize nutrient use efficiency and reduce external P inputs in sustainable agricultural systems.</div></div>","PeriodicalId":12511,"journal":{"name":"Geoderma","volume":"461 ","pages":"Article 117497"},"PeriodicalIF":6.6000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chemical imaging reveals phosphorus mobilization patterns in earthworm-engineered drilosphere\",\"authors\":\"Zhen-Yu Qiang , Dong-Xing Guan , Jia-Lu Gao , Gang Li , Daniel Menezes-Blackburn , Anna Gunina , Yvan Capowiez , Lena Q. Ma\",\"doi\":\"10.1016/j.geoderma.2025.117497\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Earthworms are soil ecosystem engineers who play a crucial role in phosphorus (P) cycling, encompassing inorganic P desorption and organic P mineralization. Although the role of earthworms in P mobilization is well documented, the spatial distribution of P mobilization processes and critical microbial species within the earthworm-engineered soil microhabitat, known as the drilosphere, remains to be determined. This study investigated P mobilization and redistribution in the drilosphere by <em>ex situ</em> chemical analyses, <em>in situ</em> soil zymography and diffusive gradients in thin-films imaging, and assessment of the microorganism communities. Endogeic earthworm species <em>Metaphire guillelmi</em> was incubated for 30 days in pots filled with soils Fluvisol or Acrisol with different total P contents (0.6 and 1.2 mg kg<sup>−1</sup>, respectively). 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引用次数: 0
摘要
蚯蚓是土壤生态系统的工程师,在磷循环中起着至关重要的作用,包括无机磷的解吸和有机磷的矿化。虽然蚯蚓在磷动员中的作用已被充分记录,但在蚯蚓工程土壤微生境(称为钻圈)中,磷动员过程和关键微生物物种的空间分布仍有待确定。本研究通过非原位化学分析、原位土壤酶谱分析、薄膜成像扩散梯度以及微生物群落评估等方法研究了土壤磷在钻井圈中的迁移和再分配。在全磷含量分别为0.6和1.2 mg kg - 1的氟维醇和阿克里醇土壤中,对内生蚯蚓进行盆栽培养,培养时间为30 d。化学分析表明,氟维醇和阿克里醇分别使蚯蚓粪便中总磷含量增加了120%和7.7%。由于酸/碱性磷酸酶活性升高和铁/铝结合磷解吸增强等原因,井圈中氟维索和阿克里索的有效磷含量分别比普通土壤高8.5倍和4.4倍。成像发现,在洞壁周围的土壤中,有效磷和酸性磷酸酶活性共存和不同的热点,热点比例为0.1% - 3.1%和5.4% - 7.5%。蚯蚓活动增加了特定细菌(气单胞菌和黄杆菌)和真菌(细孢子菌和足孢子菌)类群的丰度,可能有助于磷的动员。金属结合吸附位点的分布、磷酸酶活性和扩散机制之间的相关性有助于土壤中有效磷的再分配。这些发现为制定土壤管理策略提供了见解,这些策略可在可持续农业系统中利用蚯蚓介导的磷循环来优化养分利用效率并减少外部磷投入。
Chemical imaging reveals phosphorus mobilization patterns in earthworm-engineered drilosphere
Earthworms are soil ecosystem engineers who play a crucial role in phosphorus (P) cycling, encompassing inorganic P desorption and organic P mineralization. Although the role of earthworms in P mobilization is well documented, the spatial distribution of P mobilization processes and critical microbial species within the earthworm-engineered soil microhabitat, known as the drilosphere, remains to be determined. This study investigated P mobilization and redistribution in the drilosphere by ex situ chemical analyses, in situ soil zymography and diffusive gradients in thin-films imaging, and assessment of the microorganism communities. Endogeic earthworm species Metaphire guillelmi was incubated for 30 days in pots filled with soils Fluvisol or Acrisol with different total P contents (0.6 and 1.2 mg kg−1, respectively). Chemical analyses revealed that total P content in earthworm casts increased by 120 % in Fluvisol and 7.7 % in Acrisol. Available P content increased by 8.5 times in Fluvisol and 4.4 times in Acrisol in the drilosphere compared to bulk soil due to elevated acid/alkaline phosphatase activities and intensified Fe/Al-bound P desorption amongst others. Imaging identified co-existing and distinct hotspots for available P and acid phosphatase activity in soils surrounding the burrow walls, with hotspot proportions of 0.1 %‒3.1 % and 5.4 %‒7.5 % of the imaged areas. Earthworm activity increased the abundance of specific bacterial (Aeromonas and Flavobacterium) and fungal (Scedosporium and Podospora) taxa potentially contributing to P mobilization. The correlation between the distribution of metal-bound adsorption sites, phosphatase activity, and diffusion mechanisms contributed to the available P redistribution in the soil. These findings provide insights for developing soil management strategies that harness earthworm-mediated P cycling to optimize nutrient use efficiency and reduce external P inputs in sustainable agricultural systems.
期刊介绍:
Geoderma - the global journal of soil science - welcomes authors, readers and soil research from all parts of the world, encourages worldwide soil studies, and embraces all aspects of soil science and its associated pedagogy. The journal particularly welcomes interdisciplinary work focusing on dynamic soil processes and functions across space and time.