阿维菌素通过改变猪精子中的PKA活性和酪氨酸磷酸化来破坏精子功能

IF 2.8 4区医学 Q2 REPRODUCTIVE BIOLOGY

Reproductive toxicology Pub Date : 2025-08-27 DOI:10.1016/j.reprotox.2025.109040

Claudine Uwamahoro , Jae-Hwan Jo , Seung-Ik Jang , Eun-Ju Jung , Woo-Jin Lee , Jeong-Won Bae , Daehyun Kim , Junkoo Yi , Sangsu Shin , Joonho Moon , Woo-Sung Kwon

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引用次数: 0

摘要

广泛应用于农业的大环内酯类杀虫剂阿维菌素（ABM）对非靶生物的潜在毒性引起了人们的关注。因此，本研究旨在探讨ABM对精子功能的影响，重点关注运动性、运动学、ATP水平、活力、蛋白激酶A （PKA）活性和酪氨酸磷酸化。将猪精子与不同浓度的ABM（0、1、5、10、15、25、50和100 µM）在使能条件下孵育，并通过计算机辅助精子分析、Hoechst 33258/四环素染色、ATP和活力测定以及Western blotting进行评估。结果显示，从10 µM开始，精子活力和ATP水平呈剂量依赖性下降。在浓度至少为25 µM时，精子的获能性显著改变，获能精子增加，无获能精子减少。然而，整体细胞活力未受影响。Western blot分析显示PKA底物和酪氨酸磷酸化蛋白发生异常改变。这些发现表明，ABM破坏了精子功能和关键的受精途径，表明了对男性生育能力的潜在风险。

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Abamectin disrupts sperm function through the alteration of PKA activity and tyrosine phosphorylation in boar spermatozoa

The potential toxicity of abamectin (ABM), a macrocyclic lactone insecticide widely used in agriculture, in non-target organisms has raised concerns. Therefore, this study aimed to investigate the effects of ABM on sperm function, focusing on motility, kinematics, ATP levels, viability, protein kinase A (PKA) activity, and tyrosine phosphorylation. Boar spermatozoa were incubated with various ABM concentrations (0, 1, 5, 10, 15, 25, 50, and 100 µM) under capacitating conditions and assessed through computer-assisted sperm analysis, Hoechst 33258/chlortetracycline staining, ATP and viability assays, and Western blotting. The results revealed a dose-dependent decline in sperm motility and ATP levels starting at 10 µM. Sperm capacitation was significantly altered at concentrations of at least 25 µM, with an increase in capacitated spermatozoa and a decrease in non-capacitated spermatozoa. However, overall cell viability remained unaffected. Western blot analysis showed abnormal alterations in PKA substrates and tyrosine-phosphorylated proteins. These findings suggest ABM disrupts sperm function and key fertilization pathways, indicating potential risks to male fertility.

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来源期刊

Reproductive toxicology 生物-毒理学

CiteScore

6.50

自引率

3.00%

发文量

131

审稿时长

45 days

期刊介绍： Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.