单分子定位显微镜

IF 32.9 1区物理与天体物理 Q1 OPTICS

Nature Photonics Pub Date : 2025-09-01 DOI:10.1038/s41566-025-01724-y

Sophie Brasselet, Matthew D. Lew

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引用次数: 0

摘要

单分子定位显微镜（SMLM）在光学显微镜中提供了增强的空间分辨率，在纳米尺度上提供了对细胞中蛋白质空间组织的详细见解。在过去的十年中，SMLM逐渐纳入了利用其极化偶极发射模式检索单个分子取向的能力。在这里，我们探讨了单分子取向和定位显微镜（SMOLM）的最新进展，它产生分子三维（3D）取向、摆动和3D位置的超分辨率图像。这一进展为探索生物分子的纳米级组织和构象以及监测和设计纳米光子学中的局部3D光场开辟了可能性。我们涵盖了SMOLM的原理，讨论了生物学和光子学的最新进展和应用，最后强调了该领域令人兴奋的未来方向和挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Single-molecule orientation and localization microscopy

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Single-molecule orientation and localization microscopy

Single-molecule localization microscopy (SMLM) offers enhanced spatial resolution in optical microscopy, providing detailed insights into the spatial organization of proteins in cells at the nanoscale. Over the past decade, SMLM has progressively incorporated the capability to retrieve the orientations of single molecules using their polarized dipolar emission pattern. Here we explore recent advancements in single-molecule orientation and localization microscopy (SMOLM), which yields super-resolved images of molecular three-dimensional (3D) orientations, wobble and 3D positions. This advancement opens possibilities to explore the nanoscale organization and conformation of biological molecules as well as to monitor and design local 3D optical fields in nanophotonics. We cover the principles of SMOLM, discuss recent advances and applications in biology and photonics, and finally highlight exciting future directions and challenges in the field. The Review discusses recent advances in single-molecule orientation and localization microscopy (SMOLM) along with remaining challenges and promises for future developments of the field.

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来源期刊

Nature Photonics 物理-光学

CiteScore

54.20

自引率

1.70%

发文量

158

审稿时长

12 months

期刊介绍： Nature Photonics is a monthly journal dedicated to the scientific study and application of light, known as Photonics. It publishes top-quality, peer-reviewed research across all areas of light generation, manipulation, and detection. The journal encompasses research into the fundamental properties of light and its interactions with matter, as well as the latest developments in optoelectronic devices and emerging photonics applications. Topics covered include lasers, LEDs, imaging, detectors, optoelectronic devices, quantum optics, biophotonics, optical data storage, spectroscopy, fiber optics, solar energy, displays, terahertz technology, nonlinear optics, plasmonics, nanophotonics, and X-rays. In addition to research papers and review articles summarizing scientific findings in optoelectronics, Nature Photonics also features News and Views pieces and research highlights. It uniquely includes articles on the business aspects of the industry, such as technology commercialization and market analysis, offering a comprehensive perspective on the field.