CEBPB作为透明细胞肾细胞癌的预后生物标志物及其与免疫细胞的关系

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-09-01 DOI:10.1016/j.bbrep.2025.102231

Yaoqiang Ren , Min Wei , Quanfa Tian , Wenke Guo

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引用次数: 0

摘要

透明细胞肾细胞癌（ccRCC）是一种高度侵袭性的恶性肿瘤，预后差。本研究探讨CCAAT/增强子结合蛋白β (CEBPB)在ccRCC中的表达、预后意义和免疫关联。使用STAR工作流和R软件分析来自癌症基因组图谱（TCGA）和基因型组织表达（GTEx）项目的RNA测序数据。免疫组化（IHC）证实CEBPB在ccRCC组织中的表达。功能富集分析（基因本体和京都基因与基因组百科全书）和蛋白质相互作用（PPI）网络（STRING和Cytoscape）用于探索cebpb相关途径。单样本基因集富集分析（ssGSEA）显示CEBPB表达与24种免疫细胞浸润有显著相关性。CEBPB与免疫相关途径有关，包括体液免疫反应、白细胞迁移和细胞因子信号传导。PPI分析发现了与STAT3/EP300的强相互作用，突出了其在免疫调节中的作用。Cox回归分析显示，高CEBPB表达与较差的总生存率相关，支持其作为预后生物标志物的潜力。综上所述，CEBPB在形成ccRCC的免疫微环境中起关键作用，可能作为一种新的预后标志物和治疗靶点。

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CEBPB as a prognostic biomarker and its association with immune cells in clear cell renal cell carcinoma

Clear cell renal cell carcinoma (ccRCC) is a highly aggressive malignancy with a poor prognosis. This study examines the expression, prognostic significance, and immune association of CCAAT/enhancer-binding protein beta (CEBPB) in ccRCC. RNA sequencing data from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) Project were analyzed using the STAR workflow and R software. Immunohistochemistry (IHC) validated CEBPB expression in ccRCC tissues. Functional enrichment analyses (Gene Ontology and Kyoto Encyclopedia of Genes and Genomes) and a protein-protein interaction (PPI) network (STRING and Cytoscape) were used to explore CEBPB-related pathways. Single-sample gene set enrichment analysis (ssGSEA) revealed significant correlations between CEBPB expression and the infiltration of 24 immune cell types. CEBPB was linked to immune-related pathways, including humoral immune response, leukocyte migration, and cytokine signaling. PPI analysis identified strong interactions with STAT3/EP300, highlighting its role in immune regulation. Cox regression analysis showed that high CEBPB expression is associated with poorer overall survival, supporting its potential as a prognostic biomarker. In conclusion, CEBPB plays a key role in shaping the immune microenvironment of ccRCC and may serve as a novel prognostic marker and therapeutic target.

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.