超声辅助葡萄汁发酵的代谢组学研究

IF 9.7 1区 化学 Q1 ACOUSTICS
Liyue Fei , Dongsheng Zhang , Yu Li , Johane Johari Mkunga , Zinan Zhang , Chenglong He , Chunhui Shan , Muhammad Iqbal Choudhary , Xinquan Yang , Wenchao Cai
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引用次数: 0

摘要

大量研究表明,乳酸菌发酵和超声辅助发酵都能增强果汁的抗氧化活性;然而,这两种处理对葡萄汁(GJ)代谢产物和抗氧化活性的影响尚未研究。因此,本研究旨在分析LAB发酵葡萄汁(FGJ)和超声辅助发酵葡萄汁(UFGJ)对抗氧化活性和代谢物产生的具体影响,并利用网络药理学和分子对接技术对UFGJ抗氧化作用的潜在机制进行初步探讨。结果表明,UFGJ显著提高了FGJ和GJ的总酚含量、总黄酮含量和抗氧化活性(P < 0.001)。代谢组学分析显示,三组之间代谢物组成差异极显著(P < 0.001)。FGJ组与GJ组、UFGJ组与GJ组的主要代谢物类别和代谢途径是相同的,后者显示出更高水平的共享代谢物。因此,UFGJ并不改变FGJ的代谢途径,而是提高其代谢效率,增加代谢物的富集。相关及网络药理学分析显示,2-羟基肉桂酸、没食子酸、杨梅素3-鼠李糖苷等关键抗氧化代谢物主要靶向PPARG、EGFR、PTGS2、PPARA等核心基因,进而调控化学致癌相关的信号通路-受体活化、粘附连接、PPAR信号通路,发挥抗氧化作用。此外,分子对接实验表明熊果酸与EGFR的结合最稳定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Metabolomic insights into ultrasound-assisted fermentation of grape juice
Numerous studies have demonstrated that both lactic acid bacteria (LAB) fermentation and ultrasound-assisted fermentation can enhance the antioxidant activity of fruit juices; however, the effects of these two treatments on metabolites and antioxidant activity in grape juice (GJ) have yet to be investigated. Therefore, this study aimed to analyze the specific effects of LAB fermented grape juice (FGJ) and ultrasound-assisted fermented grape juice (UFGJ) on the antioxidant activity and metabolite production, while also conducting a preliminary investigation into the potential mechanisms underlying the antioxidant action of UFGJ using network pharmacology and molecular docking. The results indicated that UFGJ significantly enhanced the total phenolic content, total flavonoid content, and antioxidant activity of both FGJ and GJ (P < 0.001). Metabolomics analysis revealed highly significant differences in metabolite composition among the three groups (P < 0.001). The major differential metabolite classes and metabolic pathways of the FGJ versus GJ and UFGJ versus GJ groups were the same, with the latter group showing higher levels of shared metabolites. Thus, UFGJ does not alter the metabolic pathway of FGJ, but instead enhances its metabolic efficiency and increases metabolite enrichment. Correlation and network pharmacology analyses revealed that key antioxidant metabolites such as 2-hydroxycinnamic acid, gallic acid, and myricetin 3-rhamnoside primarily target core genes such as PPARG, EGFR, PTGS2, and PPARA, further regulating signaling pathways associated with chemical carcinogenesis-receptor activation, adherens junctions, and PPAR signaling pathway to exert antioxidant effects. Furthermore, molecular docking assays demonstrated that ursolic acid displaying the most stable binding to EGFR.
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来源期刊
Ultrasonics Sonochemistry
Ultrasonics Sonochemistry 化学-化学综合
CiteScore
15.80
自引率
11.90%
发文量
361
审稿时长
59 days
期刊介绍: Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.
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