Zhao Li, Bushra Tousian, Michal Zaiden, Ishani Sarkar, Clark Vu, Yin Wang*, Ronit Bitton* and John B. Matson*,
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Cellular Uptake and Antioxidant Activity of H2S-Releasing Tetrapeptide Supramolecular Polymer Nanostructures
Self-assembled peptide–H2S donor conjugates (PHDCs) can deliver hydrogen sulfide in vitro and in vivo, yet the link between the supramolecular nanostructure morphology and cellular uptake remains unclear. Herein, we designed constitutionally isomeric PHDCs that self-assembled in aqueous solution into either nanoribbons, nanofibers, or nanobelts with various dimensions based on cryogenic transmission electron microscopy and small-angle X-ray scattering. Nile-red loaded PHDCs showed morphology-dependent uptake by H9C2 cells based on fluorescence microscopy combined with flow cytometry and confocal imaging, where narrow, helically twisted nanoribbons entered most efficiently. All PHDCs released H2S at similar rates, but the amount of H2S released inside the cells depended on the internalization ability of each PHDC. Consistent with these results, the narrow twisted nanoribbons afforded the greatest protection against H2O2-induced oxidative stress. Overall, this study highlights how subtle molecular-level changes can influence nanostructure formation in supramolecular assemblies and ultimately affect their cellular uptake and biological activities.
期刊介绍：
ACS Materials Letters is a journal that publishes high-quality and urgent papers at the forefront of fundamental and applied research in the field of materials science. It aims to bridge the gap between materials and other disciplines such as chemistry, engineering, and biology. The journal encourages multidisciplinary and innovative research that addresses global challenges. Papers submitted to ACS Materials Letters should clearly demonstrate the need for rapid disclosure of key results. The journal is interested in various areas including the design, synthesis, characterization, and evaluation of emerging materials, understanding the relationships between structure, property, and performance, as well as developing materials for applications in energy, environment, biomedical, electronics, and catalysis. The journal has a 2-year impact factor of 11.4 and is dedicated to publishing transformative materials research with fast processing times. The editors and staff of ACS Materials Letters actively participate in major scientific conferences and engage closely with readers and authors. The journal also maintains an active presence on social media to provide authors with greater visibility.
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