薄层色谱生物自扫描电镜（TLCBA SEM）检测抗菌成分的方法

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-08-27 DOI:10.1016/j.mimet.2025.107242

Anna du Toit , Chaitali Dekiwadia , Lisa Cornell , Diane Purcell , Anusuya Willis , Rajaraman Eri , Danilla Grando , Charmaine Lloyd

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引用次数: 0

摘要

采用薄层色谱生物自显影（TLCBA）和扫描电镜（SEM）相结合的方法，观察了色谱组分对细菌形态的影响。覆盖在已知抗葡萄球菌分数CS1上的盖片可以直接处理用于SEM测试，其结果与需要更多测试化合物和时间的平板稀释方法相似。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Thin Layer Chromatography Bioautography Scanning Electron Microscopy (TLCBA SEM) method for antimicrobial fraction testing

Thin Layer Chromatography Bio-autography (TLCBA) and Scanning Electron Microscopy (SEM) have been combined to observe the effect of chromatographed fractions on bacterial morphology. Coverslips overlaid on known anti-staphylococcal fraction CS1 could be processed directly for SEM testing, giving similar results to plate-dilution methods which require more test compounds and time.

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.