Catalytic performance of lipase-inorganic hybrid nanoflower in the presence of some oils/fat/waste oils and detergents/surfactants

In this study, the conventional hybrid nanoflower synthesis method was used for lipase-inorganic hybrid nanoflowers. Following a comprehensive pH and temperature scanning of the nanoflowers, their activity was investigated in some detergents/surfactants and oils/fats/waste oils to evaluate the activity, stability and compatibility of the biocatalyst. The optimal morphology for L@ihNFs was determined to be 0.05 mg/mL. To obtain the optimum activity, conditions were explored pH values ranging from 6 to 12 and temperatures between 30 and 80 °C. Both the free form and L@ihNF exhibited higher activity in alkaline conditions, with a significant decrease in activity observed at pH 11 and 12. L@ihNF showed superior activity and remarkable compatibility compared to the free enzyme in the presence of various commercial liquid/powder detergents and surfactants. Moreover, it displayed over a twofold increase in activity in some oils/fat/waste oils. The reusability of the synthesized L@ihNF was examined in 10 cycles, and it was observed that it still showed 58% activity at the end of the 8th cycle, which is a good indicator of reusability. L@ihNFs exhibited good storage stability, retaining approximately 50% activity after 20 days compared to the free enzyme.