分析特定挥发性有机化合物的霉菌检测和物种鉴定

IF 4.3 2区 环境科学与生态学 Q1 CONSTRUCTION & BUILDING TECHNOLOGY
Indoor air Pub Date : 2025-08-29 DOI:10.1155/ina/9350601
Linduo Zhao, Clint M. Arnett, Veronica I. Casey, John W. Scott, Mark A. Davis, Gwenna Heidkamp, Rebekah C. Wilson
{"title":"分析特定挥发性有机化合物的霉菌检测和物种鉴定","authors":"Linduo Zhao,&nbsp;Clint M. Arnett,&nbsp;Veronica I. Casey,&nbsp;John W. Scott,&nbsp;Mark A. Davis,&nbsp;Gwenna Heidkamp,&nbsp;Rebekah C. Wilson","doi":"10.1155/ina/9350601","DOIUrl":null,"url":null,"abstract":"<p>Mold is found in most indoor environments and is of great concern due to adverse health effects and infrastructure damage it can cause. One key aspect of this growing problem is early detection and localization of mold contamination so appropriate measures can be implemented. In this study, a combination of solid-phase microextraction (SPME), semiquantitative gas chromatography–mass spectroscopy (GC-MS) analysis, and principal component analysis (PCA) of 14 select microbial volatile organic compounds (MVOCs) was used to determine if volatile organic profiling could be used to differentiate between molds grown on various building materials. Briefly, SPME fibers with PDMS/DVB coatings were employed to collect and generate volatile organic profiles of target MVOCs emitted by <i>Aspergillus versicolor</i> and <i>Penicillium chrysogenum</i> when grown on two common building materials. The volatile compound extraction and identification method revealed that <i>P. chrysogenum</i> grown on gypsum and <i>A. versicolor</i> grown on pine produced unique MVOC profiles from one another, which indicated species and substrate differentiation could be made based on the volatile organic profiles. Additionally, the production of dimethyl disulfide (DMDS) and geosmin was found to be specific to <i>P. chrysogenum</i> and <i>A. versicolor</i>, respectively, and therefore could serve as potential biomarkers for screening for the presence of each species. This study suggests profiling select MVOCs is viable for detecting specific hazardous molds when the substrate is known and a streamlined workflow for indoor mold monitoring: initial broad-spectrum GC-MS screening for fungal presence → selected MVOC profiling for species identification → molecular verification of hazardous species.</p>","PeriodicalId":13529,"journal":{"name":"Indoor air","volume":"2025 1","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/ina/9350601","citationCount":"0","resultStr":"{\"title\":\"Profiling Specific Volatile Organic Compounds for Mold Detection and Species Identification\",\"authors\":\"Linduo Zhao,&nbsp;Clint M. Arnett,&nbsp;Veronica I. Casey,&nbsp;John W. Scott,&nbsp;Mark A. Davis,&nbsp;Gwenna Heidkamp,&nbsp;Rebekah C. Wilson\",\"doi\":\"10.1155/ina/9350601\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Mold is found in most indoor environments and is of great concern due to adverse health effects and infrastructure damage it can cause. One key aspect of this growing problem is early detection and localization of mold contamination so appropriate measures can be implemented. In this study, a combination of solid-phase microextraction (SPME), semiquantitative gas chromatography–mass spectroscopy (GC-MS) analysis, and principal component analysis (PCA) of 14 select microbial volatile organic compounds (MVOCs) was used to determine if volatile organic profiling could be used to differentiate between molds grown on various building materials. Briefly, SPME fibers with PDMS/DVB coatings were employed to collect and generate volatile organic profiles of target MVOCs emitted by <i>Aspergillus versicolor</i> and <i>Penicillium chrysogenum</i> when grown on two common building materials. The volatile compound extraction and identification method revealed that <i>P. chrysogenum</i> grown on gypsum and <i>A. versicolor</i> grown on pine produced unique MVOC profiles from one another, which indicated species and substrate differentiation could be made based on the volatile organic profiles. Additionally, the production of dimethyl disulfide (DMDS) and geosmin was found to be specific to <i>P. chrysogenum</i> and <i>A. versicolor</i>, respectively, and therefore could serve as potential biomarkers for screening for the presence of each species. This study suggests profiling select MVOCs is viable for detecting specific hazardous molds when the substrate is known and a streamlined workflow for indoor mold monitoring: initial broad-spectrum GC-MS screening for fungal presence → selected MVOC profiling for species identification → molecular verification of hazardous species.</p>\",\"PeriodicalId\":13529,\"journal\":{\"name\":\"Indoor air\",\"volume\":\"2025 1\",\"pages\":\"\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2025-08-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1155/ina/9350601\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indoor air\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1155/ina/9350601\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CONSTRUCTION & BUILDING TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indoor air","FirstCategoryId":"93","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/ina/9350601","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CONSTRUCTION & BUILDING TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

霉菌存在于大多数室内环境中,由于其对健康的不良影响和对基础设施的破坏,引起了人们的极大关注。这个日益严重的问题的一个关键方面是早期发现和定位霉菌污染,以便采取适当的措施。本研究采用固相微萃取(SPME)、半定量气相色谱-质谱(GC-MS)和主成分分析(PCA)相结合的方法对14种微生物挥发性有机化合物(MVOCs)进行分析,以确定挥发性有机分析是否可以用于区分生长在不同建筑材料上的霉菌。简单地说,采用涂有PDMS/DVB涂层的SPME纤维,收集并生成了在两种常见建筑材料上生长的花式曲霉和青霉菌释放的目标MVOCs的挥发性有机特征。挥发性化合物的提取和鉴定方法表明,生长在石膏上的黄皮草和生长在松木上的花斑草产生了不同的MVOC谱,这表明可以根据挥发性有机谱进行物种和底物的区分。此外,还发现二甲基二硫化物(DMDS)和土臭素(geosmin)的产生分别对P. chrysogenum和A. versicolor具有特异性,因此可以作为筛选每种物种存在的潜在生物标志物。该研究表明,当底物已知时,分析选定的MVOC对于检测特定的有害霉菌是可行的,并且简化了室内霉菌监测的工作流程:初始广谱GC-MS筛选真菌存在→选择MVOC分析用于物种鉴定→有害物种的分子验证。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Profiling Specific Volatile Organic Compounds for Mold Detection and Species Identification

Profiling Specific Volatile Organic Compounds for Mold Detection and Species Identification

Mold is found in most indoor environments and is of great concern due to adverse health effects and infrastructure damage it can cause. One key aspect of this growing problem is early detection and localization of mold contamination so appropriate measures can be implemented. In this study, a combination of solid-phase microextraction (SPME), semiquantitative gas chromatography–mass spectroscopy (GC-MS) analysis, and principal component analysis (PCA) of 14 select microbial volatile organic compounds (MVOCs) was used to determine if volatile organic profiling could be used to differentiate between molds grown on various building materials. Briefly, SPME fibers with PDMS/DVB coatings were employed to collect and generate volatile organic profiles of target MVOCs emitted by Aspergillus versicolor and Penicillium chrysogenum when grown on two common building materials. The volatile compound extraction and identification method revealed that P. chrysogenum grown on gypsum and A. versicolor grown on pine produced unique MVOC profiles from one another, which indicated species and substrate differentiation could be made based on the volatile organic profiles. Additionally, the production of dimethyl disulfide (DMDS) and geosmin was found to be specific to P. chrysogenum and A. versicolor, respectively, and therefore could serve as potential biomarkers for screening for the presence of each species. This study suggests profiling select MVOCs is viable for detecting specific hazardous molds when the substrate is known and a streamlined workflow for indoor mold monitoring: initial broad-spectrum GC-MS screening for fungal presence → selected MVOC profiling for species identification → molecular verification of hazardous species.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Indoor air
Indoor air 环境科学-工程:环境
CiteScore
10.80
自引率
10.30%
发文量
175
审稿时长
3 months
期刊介绍: The quality of the environment within buildings is a topic of major importance for public health. Indoor Air provides a location for reporting original research results in the broad area defined by the indoor environment of non-industrial buildings. An international journal with multidisciplinary content, Indoor Air publishes papers reflecting the broad categories of interest in this field: health effects; thermal comfort; monitoring and modelling; source characterization; ventilation and other environmental control techniques. The research results present the basic information to allow designers, building owners, and operators to provide a healthy and comfortable environment for building occupants, as well as giving medical practitioners information on how to deal with illnesses related to the indoor environment.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信