人畜共患副痘病毒分离株的分子特性及其生长特性

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-08-23 DOI:10.1016/j.genrep.2025.102328

Gizem Aytoğu , Mevlüt Yaşar , Nilay Aybey , Zafer Mecitoğlu , Kadir Yeşilbağ

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引用次数: 0

摘要

副痘病毒（ppv），包括牛丘疹性口炎病毒（BPSV）、假牛痘病毒（PCPV）和ORF病毒（ORFV），是影响野生和家养反刍动物的人畜共患病原体。在2023年至2024年期间，土耳其各地区报告了小牛嘴唇、鼻子和舌头上的糜烂丘疹和溃疡，以及羔羊的增殖性口腔病变。在两个地理上相距遥远的肉牛群中，动物处理人员的手部结节性病变表明人畜共患病。用B2L基因特异性引物对疑似样本进行PCR鉴定。对代表BPSV、PCPV和ORFV的三个分离株进行测序并与全球数据进行比较。PCPV与来自孟加拉国和芬兰的毒株最相似，而BPSV与来自中国和伊朗的毒株最相似。在四种细胞系：原代胎羊肾（PLK）、马丁-达比牛肾（MDBK）、羊胎胸腺（SFT-R）和非洲绿猴肾（VERO）上尝试分离病毒。PLK细胞对这三种病毒的分离成功率均为100%。在连续传代中，ORFV在PLK细胞中复制最好，始终产生最高的病毒滴度。本研究提供了目前在 rkiye中流行的人畜共患ppv的分子和系统发育特征，并比较了它们的体外复制效率。PLK细胞被认为是最敏感和多产的系统，特别是ORFV，由于其免疫调节潜力而引起人们的兴趣。

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Molecular characterization of current field isolates of zoonotic parapoxviruses and their growth characteristics

Parapoxviruses (PPVs), including Bovine papular stomatitis virus (BPSV), Pseudocowpox virus (PCPV), and ORF virus (ORFV), are zoonotic pathogens affecting wild and domesticated ruminants. Between 2023 and 2024, erosive papules and ulcers on the lips, nose, and tongue of calves, as well as proliferative oral lesions in lambs, were reported in various Turkish regions. In two geographically distant beef herds, nodular hand lesions in animal handlers indicated zoonotic transmission. Suspected samples were confirmed by PCR using B2L gene-specific primers. Three isolates representing BPSV, PCPV, and ORFV were sequenced and compared to global data. PCPV showed closest similarity to strains from Bangladesh and Finland, while BPSV was most similar to strains from China and Iran. Virus isolation was attempted on four cell lines: primary fetal lamb kidney (PLK), Madin-Darby bovine kidney (MDBK), sheep fetal thymus (SFT-R), and African green monkey kidney (VERO). PLK cells showed 100 % isolation success for all three viruses. In serial passages, ORFV replicated best in PLK cells, consistently yielding the highest viral titers. This study provides molecular and phylogenetic characterization of currently circulating zoonotic PPVs in Türkiye and compares their in vitro replication efficiency. PLK cells were identified as the most sensitive and productive system, particularly for ORFV, which is of interest due to its immunomodulatory potential.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.