Zhiyong Zhang, Shuangshuang Du, Shuangyi Li, Bingxue Li, Jingkuan Wang
{"title":"覆盖和增施氮肥对土壤微食物网的影响","authors":"Zhiyong Zhang, Shuangshuang Du, Shuangyi Li, Bingxue Li, Jingkuan Wang","doi":"10.1016/j.geoderma.2025.117487","DOIUrl":null,"url":null,"abstract":"<div><div>Film mulching and increased nitrogen fertilization profoundly affect soil ecosystem functioning and stability. The soil micro-food web, comprising diverse nematode-microbe interactions, plays a crucial role in mediating nutrient exchanges and energy flows. However, the effects of plastic film mulching (PFM) and elevated nitrogen fertilization on soil micro-food web dynamics remain poorly understood. In a long-term field trial, we evaluated these impacts using a two-factor design with mulching conditions (with (+PFM) and without (−PFM) plastic film) and three nitrogen fertilizer dosages (0 (N<sub>0</sub>), 135 (N<sub>135</sub>), and 270 kg N ha<sup>−1</sup> yr<sup>−1</sup> (N<sub>270</sub>)). Soil sampling was conducted at three maize growth stages: seedling, jointing, and filling. The results revealed that +PFM led to notable declines in species richness and ecological network connectivity within the soil micro-food web, compared to −PFM. Compared to N<sub>270</sub>, N<sub>0</sub> resulted in a markedly greater biomass carbon content in the soil micro-food web. A significantly lower soil micro-food web connectance was observed in N<sub>270</sub> than in N<sub>0</sub>. The nematode channel ratio was significantly higher under N<sub>270</sub> than under N<sub>0</sub>. Principal component analysis demonstrated that nitrogen fertilization intensity significantly altered soil physicochemical properties. Redundancy analysis revealed that soil temperature and ammonium nitrogen were the dominant factors driving micro-food web restructuring. Structural equation modeling clarified that the alteration of soil micro-food web under plastic film mulching was driven by fluctuations in soil temperature and ammonium nitrogen status. High nitrogen fertilizer input suppressed micro-food web development via ammonium nitrogen accumulation. Overall, long-term plastic film mulching and excessive nitrogen fertilization adversely affect the diversity, stability, and ecological functioning of soil biotic communities.</div></div>","PeriodicalId":12511,"journal":{"name":"Geoderma","volume":"461 ","pages":"Article 117487"},"PeriodicalIF":6.6000,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Impacts of film mulching and increased nitrogen fertilization on the soil micro-food web\",\"authors\":\"Zhiyong Zhang, Shuangshuang Du, Shuangyi Li, Bingxue Li, Jingkuan Wang\",\"doi\":\"10.1016/j.geoderma.2025.117487\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Film mulching and increased nitrogen fertilization profoundly affect soil ecosystem functioning and stability. The soil micro-food web, comprising diverse nematode-microbe interactions, plays a crucial role in mediating nutrient exchanges and energy flows. However, the effects of plastic film mulching (PFM) and elevated nitrogen fertilization on soil micro-food web dynamics remain poorly understood. In a long-term field trial, we evaluated these impacts using a two-factor design with mulching conditions (with (+PFM) and without (−PFM) plastic film) and three nitrogen fertilizer dosages (0 (N<sub>0</sub>), 135 (N<sub>135</sub>), and 270 kg N ha<sup>−1</sup> yr<sup>−1</sup> (N<sub>270</sub>)). Soil sampling was conducted at three maize growth stages: seedling, jointing, and filling. The results revealed that +PFM led to notable declines in species richness and ecological network connectivity within the soil micro-food web, compared to −PFM. Compared to N<sub>270</sub>, N<sub>0</sub> resulted in a markedly greater biomass carbon content in the soil micro-food web. A significantly lower soil micro-food web connectance was observed in N<sub>270</sub> than in N<sub>0</sub>. The nematode channel ratio was significantly higher under N<sub>270</sub> than under N<sub>0</sub>. Principal component analysis demonstrated that nitrogen fertilization intensity significantly altered soil physicochemical properties. Redundancy analysis revealed that soil temperature and ammonium nitrogen were the dominant factors driving micro-food web restructuring. Structural equation modeling clarified that the alteration of soil micro-food web under plastic film mulching was driven by fluctuations in soil temperature and ammonium nitrogen status. High nitrogen fertilizer input suppressed micro-food web development via ammonium nitrogen accumulation. Overall, long-term plastic film mulching and excessive nitrogen fertilization adversely affect the diversity, stability, and ecological functioning of soil biotic communities.</div></div>\",\"PeriodicalId\":12511,\"journal\":{\"name\":\"Geoderma\",\"volume\":\"461 \",\"pages\":\"Article 117487\"},\"PeriodicalIF\":6.6000,\"publicationDate\":\"2025-08-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Geoderma\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0016706125003283\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"SOIL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Geoderma","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0016706125003283","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SOIL SCIENCE","Score":null,"Total":0}
引用次数: 0
摘要
覆膜和增施氮肥对土壤生态系统功能和稳定性有深远影响。土壤微食物网由多种线虫-微生物相互作用组成,在调节养分交换和能量流动方面起着至关重要的作用。然而,地膜覆盖和高氮肥对土壤微食物网动态的影响尚不清楚。在长期的田间试验中,我们使用双因素设计评估了这些影响,包括覆盖条件(使用(+PFM)和不使用(- PFM)塑料薄膜)和三种氮肥用量(0 (N0), 135 (N135)和270 kg N ha - 1年- 1 (N270))。在玉米苗期、拔节期和灌浆期三个生育期进行土壤取样。结果表明,与−PFM相比,+PFM导致土壤微食物网物种丰富度和生态网络连通性显著下降。与N270相比,N0显著提高了土壤微食物网的生物量碳含量。N270土壤微食物网连通性显著低于N0。N270处理下线虫通道比率显著高于N0处理。主成分分析表明,施氮强度显著改变了土壤理化性质。冗余分析表明,土壤温度和铵态氮是微食物网重构的主导因素。结构方程模型表明,地膜覆盖下土壤微食物网的变化受土壤温度和铵态氮状态波动的驱动。高氮肥投入通过铵态氮积累抑制微食物网发育。总体而言,长期地膜覆盖和过量施氮对土壤生物群落的多样性、稳定性和生态功能产生不利影响。
Impacts of film mulching and increased nitrogen fertilization on the soil micro-food web
Film mulching and increased nitrogen fertilization profoundly affect soil ecosystem functioning and stability. The soil micro-food web, comprising diverse nematode-microbe interactions, plays a crucial role in mediating nutrient exchanges and energy flows. However, the effects of plastic film mulching (PFM) and elevated nitrogen fertilization on soil micro-food web dynamics remain poorly understood. In a long-term field trial, we evaluated these impacts using a two-factor design with mulching conditions (with (+PFM) and without (−PFM) plastic film) and three nitrogen fertilizer dosages (0 (N0), 135 (N135), and 270 kg N ha−1 yr−1 (N270)). Soil sampling was conducted at three maize growth stages: seedling, jointing, and filling. The results revealed that +PFM led to notable declines in species richness and ecological network connectivity within the soil micro-food web, compared to −PFM. Compared to N270, N0 resulted in a markedly greater biomass carbon content in the soil micro-food web. A significantly lower soil micro-food web connectance was observed in N270 than in N0. The nematode channel ratio was significantly higher under N270 than under N0. Principal component analysis demonstrated that nitrogen fertilization intensity significantly altered soil physicochemical properties. Redundancy analysis revealed that soil temperature and ammonium nitrogen were the dominant factors driving micro-food web restructuring. Structural equation modeling clarified that the alteration of soil micro-food web under plastic film mulching was driven by fluctuations in soil temperature and ammonium nitrogen status. High nitrogen fertilizer input suppressed micro-food web development via ammonium nitrogen accumulation. Overall, long-term plastic film mulching and excessive nitrogen fertilization adversely affect the diversity, stability, and ecological functioning of soil biotic communities.
期刊介绍:
Geoderma - the global journal of soil science - welcomes authors, readers and soil research from all parts of the world, encourages worldwide soil studies, and embraces all aspects of soil science and its associated pedagogy. The journal particularly welcomes interdisciplinary work focusing on dynamic soil processes and functions across space and time.