视网膜蛋白质组学分析揭示了豚鼠近视模型中开/关视觉刺激特异性变化

IF 2.7 2区医学 Q1 OPHTHALMOLOGY

Experimental eye research Pub Date : 2025-08-25 DOI:10.1016/j.exer.2025.110599

Qin Dai , Qiqi Xie , Frank Schaeffel , Sisi Zheng , Qi Sun , Jinfei Wei , Bingyan Shen , Yenan Fang , Ziwei Li , Xi Chen , Xiangtian Zhou , Min Wang

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引用次数: 0

摘要

本研究旨在探讨人工动态开/关刺激对豚鼠近视发展及相关视网膜蛋白质组学变化的影响，并阐明其潜在机制。使用- 4屈光度透镜诱导豚鼠一只眼近视，另一只眼作为未治疗对照。53只豚鼠随机给予平衡的开/关（自然对照，NC）、开或关刺激，持续7天。测量了轴向长度和折射率。每组4只豚鼠采用he染色法测定脉络膜厚度。采用LC-MS/MS分析视网膜蛋白质组，比较各组蛋白表达情况。与NC近视眼相比，ON和OFF刺激的近视眼的近视程度较低。HE染色显示NC型近视眼脉络膜较薄；在开/关组，两只眼睛之间没有差异。在ON、OFF和NC组共观察到646个差异表达蛋白（DEPs）。ON/ off特异性DEPs影响糖代谢和RNA功能。在ON/NC dep中，Rap1信号通路活性降低。GZMK、NDFIP2、PARP12、ZC3HAV1和ID1被鉴定为不受视觉刺激影响的常见蛋白，但仅通过负晶状体暴露诱导近视。这三组的dep也与人类近视相关的蛋白质重叠。综上所述，ON和OFF刺激对豚鼠近视有抑制作用，并影响独特的dep；Rap1信号通路可能是近视干预的靶点；而跨群体常见的dep与氧化应激和炎症有关。registrationwydw2023-0063/2023.3.5审判。

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Retinal proteomic analysis reveals ON/OFF visual stimulation-specific changes in a Guinea pig myopia model

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Retinal proteomic analysis reveals ON/OFF visual stimulation-specific changes in a Guinea pig myopia model

This study aimed to explore the effect of artificial dynamic ON/OFF stimulation on the development of myopia and associated retinal proteomic changes in guinea pigs, and elucidate the underlying mechanisms. Myopia was induced in one eye of guinea pigs using −4 diopters lenses, with the other eye used as the untreated control. 53 guinea pigs were randomly given balanced ON/OFF (natural control, NC), ON, or OFF stimulation for 7 days. The axial lengths and refractions were measured. The choroidal thickness was measured using HE-staining in 4 guinea pigs per group. The retinal proteome was analyzed by LC-MS/MS, and protein expression compared across groups. Myopic eyes exposed to ON and OFF stimulation exhibited a lower degree of myopia compared with NC myopic eyes. HE staining revealed that NC myopic eyes had a thinner choroid; there was no difference between the two eyes in the ON/OFF group. A total of 646 differentially expressed proteins (DEPs) were observed in the ON, OFF, and NC groups. ON/OFF-specific DEPs affected glycometabolism and RNA function. Reduced activity of the Rap1 signaling pathway was enriched in the both ON/NC DEPs. GZMK, NDFIP2, PARP12, ZC3HAV1, and ID1 were identified as common proteins that remained unaffected by visual stimulations but were implicated in myopia induction solely through negative lens exposure. The DEPs in the three groups also overlapped with proteins associated with human myopia. In conclusion, ON and OFF stimulation suppressed myopia in guinea pigs, with unique DEPs affected; the Rap1 signaling pathway may be a myopia intervention target; and DEPs common across groups are related to oxidative stress and inflammation.

Trial registration

wydw2023-0063/2023.3.5.

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来源期刊

Experimental eye research 医学-眼科学

CiteScore

6.80

自引率

5.90%

发文量

323

审稿时长

66 days

期刊介绍： The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.