健康成人外周血单个核细胞生成CHOPi014-A

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-08-24 DOI:10.1016/j.scr.2025.103820

Alyssa L. Gagne , Jean Ann Maguire , Lea Dungan , Paul Gadue , Deborah L. French , Giulia Pavani

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引用次数: 0

摘要

CHOPWT15是一种对照雄性诱导多能干细胞（iPSC）系，可通过使用基因组编辑技术创建等位基因系列来模拟遗传变异（Hockemeyer和Jaenisch， 2016， Maguire等人，2022，Pavani等人，2022）。iPSC系的等位基因系列提供了对病理变异进行基因型和表型分析的手段，而没有遗传背景的混淆效应。使用仙台病毒对从健康成年男性获得的外周血单个核细胞（PBMCs）进行重编程，创建具有不可检测的拷贝数变异（CNVs，分辨率= 100 kb）的iPSC系，并向所有三种种层分化。

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Generation of CHOPi014-A from healthy adult peripheral blood mononuclear cells

CHOPWT15 is a control male induced pluripotent stem cell (iPSC) line that can be used to model genetic variants by creating an allelic series of isogenic lines using genome editing technologies (Hockemeyer and Jaenisch, 2016, Maguire et al., 2022, Pavani et al., 2022). An allelic series of iPSC lines provides the means to perform genotype and phenotype analyses of pathological variants without the confounding effects of genetic background. Peripheral blood mononuclear cells (PBMCs), obtained from a healthy adult male, were reprogrammed using Sendai virus creating an iPSC line that has undetectable copy number variations (CNVs, resolution >= 100 kb) and differentiates to all three germlayers.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.