Harnessing a versatile monooxygenase GorA to synthesize N-hydroxy compounds

In Cell Reports Physical Science, Maier et al. unveil a biocatalytic approach for synthesizing N-hydroxy compounds by integrating the flavin-dependent monooxygenase GorA into an enzymatic cascade with the decarboxylase GorB and a formate dehydrogenase-driven cofactor recycling system. This work showcases GorA’s substrate scope and establishes a biocatalytic synthetic route for valuable N-hydroxy compounds.