I. N. Rybolovlev, M. M. Rudenok, E. I. Semenova, M. V. Shulskaya, S. A. Partevyan, M. V. Lukashevich, L. I. Davydova, M. A. Klinskaya, V. G. Bogush, E. L. Arsenyeva, L. V. Novosadova, E. V. Novosadova, M. I. Shadrina, P. A. Slominsky
{"title":"重组蜘蛛蛋白rS1/9微凝胶对帕金森病Mptp模型小鼠黑质多巴胺能神经元数量的部分恢复","authors":"I. N. Rybolovlev, M. M. Rudenok, E. I. Semenova, M. V. Shulskaya, S. A. Partevyan, M. V. Lukashevich, L. I. Davydova, M. A. Klinskaya, V. G. Bogush, E. L. Arsenyeva, L. V. Novosadova, E. V. Novosadova, M. I. Shadrina, P. A. Slominsky","doi":"10.1134/S2635167625600671","DOIUrl":null,"url":null,"abstract":"<p>It has been previously shown that on a three-dimensional microgel matrix based on recombinant spidroin rS1/9, induced pluripotent stem cells effectively differentiate into a heterogeneous cell population enriched with dopaminergic neurons. An analysis of the functional maturity and activity of the obtained neuronal cultures is carried out, for which they are stereotaxically in vivo administered to mice with a toxin-induced model of Parkinson’s disease. It is shown that the introduction of all analyzed cell-culture variants leads to an increase in the number of dopaminergic neurons and an increase in the proportion of this type of cells in the total cell pool in substantia nigra compacta. This increase is already observed upon the introduction of a microgel based on a mixture of recombinant spidroin proteins rS1/9 and rS2/12 in a ratio of 1 : 1 with 10% heparin-binding peptide and RGDS peptide.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":"20 3","pages":"353 - 358"},"PeriodicalIF":0.8000,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Partial Restoration of the Number of Dopaminergic Neurons in the Substantia Nigra in Mice with the Mptp Model of Parkinson’s Disease by Introducing a Microgel Based on Recombinant Spidroin rS1/9\",\"authors\":\"I. N. Rybolovlev, M. M. Rudenok, E. I. Semenova, M. V. Shulskaya, S. A. Partevyan, M. V. Lukashevich, L. I. Davydova, M. A. Klinskaya, V. G. Bogush, E. L. Arsenyeva, L. V. Novosadova, E. V. Novosadova, M. I. Shadrina, P. A. Slominsky\",\"doi\":\"10.1134/S2635167625600671\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>It has been previously shown that on a three-dimensional microgel matrix based on recombinant spidroin rS1/9, induced pluripotent stem cells effectively differentiate into a heterogeneous cell population enriched with dopaminergic neurons. An analysis of the functional maturity and activity of the obtained neuronal cultures is carried out, for which they are stereotaxically in vivo administered to mice with a toxin-induced model of Parkinson’s disease. It is shown that the introduction of all analyzed cell-culture variants leads to an increase in the number of dopaminergic neurons and an increase in the proportion of this type of cells in the total cell pool in substantia nigra compacta. This increase is already observed upon the introduction of a microgel based on a mixture of recombinant spidroin proteins rS1/9 and rS2/12 in a ratio of 1 : 1 with 10% heparin-binding peptide and RGDS peptide.</p>\",\"PeriodicalId\":716,\"journal\":{\"name\":\"Nanotechnologies in Russia\",\"volume\":\"20 3\",\"pages\":\"353 - 358\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2025-08-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nanotechnologies in Russia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S2635167625600671\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Engineering\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanotechnologies in Russia","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1134/S2635167625600671","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Engineering","Score":null,"Total":0}
Partial Restoration of the Number of Dopaminergic Neurons in the Substantia Nigra in Mice with the Mptp Model of Parkinson’s Disease by Introducing a Microgel Based on Recombinant Spidroin rS1/9
It has been previously shown that on a three-dimensional microgel matrix based on recombinant spidroin rS1/9, induced pluripotent stem cells effectively differentiate into a heterogeneous cell population enriched with dopaminergic neurons. An analysis of the functional maturity and activity of the obtained neuronal cultures is carried out, for which they are stereotaxically in vivo administered to mice with a toxin-induced model of Parkinson’s disease. It is shown that the introduction of all analyzed cell-culture variants leads to an increase in the number of dopaminergic neurons and an increase in the proportion of this type of cells in the total cell pool in substantia nigra compacta. This increase is already observed upon the introduction of a microgel based on a mixture of recombinant spidroin proteins rS1/9 and rS2/12 in a ratio of 1 : 1 with 10% heparin-binding peptide and RGDS peptide.
期刊介绍:
Nanobiotechnology Reports publishes interdisciplinary research articles on fundamental aspects of the structure and properties of nanoscale objects and nanomaterials, polymeric and bioorganic molecules, and supramolecular and biohybrid complexes, as well as articles that discuss technologies for their preparation and processing, and practical implementation of products, devices, and nature-like systems based on them. The journal publishes original articles and reviews that meet the highest scientific quality standards in the following areas of science and technology studies: self-organizing structures and nanoassemblies; nanostructures, including nanotubes; functional and structural nanomaterials; polymeric, bioorganic, and hybrid nanomaterials; devices and products based on nanomaterials and nanotechnology; nanobiology and genetics, and omics technologies; nanobiomedicine and nanopharmaceutics; nanoelectronics and neuromorphic computing systems; neurocognitive systems and technologies; nanophotonics; natural science methods in a study of cultural heritage items; metrology, standardization, and monitoring in nanotechnology.