{"title":"马尾鱼培养中干酪乳杆菌的功能活性研究","authors":"Yin Han, Duoxiang Zhao, Da Liang, Yuanyuan Sun, Wenjie Wang, Jiansong Chu, Zizhong Qi","doi":"10.1007/s10499-025-02189-0","DOIUrl":null,"url":null,"abstract":"<div><p>This study investigated the survival dynamics of <i>Lacticaseibacillus casei</i> YB-38 in nutrient-rich seawater and its inhibitory effect on the pathogenic bacterium <i>Vibrio harveyi</i> YL-10. <i>L. casei</i> YB-38 at 10<sup>8</sup> CFU per gram of feed was supplemented with feed in the cultivation of <i>Oplegnathus punctatus</i> (initial average weight = 5.30 ± 0.20 g), focusing on the growth characteristics for 14 days. Flow cytometry and genomics analysis were utilized to investigate the effects of <i>L. casei</i> YB-38 on the intestinal microbiota activity and immune function of <i>O. punctatus</i>. The results indicated that <i>L. casei</i> YB-38 and <i>V. harveyi</i> YL-10 demonstrate enhanced growth under simulated enriched conditions. <i>V. harveyi</i> YL-10 exhibited better growth when attaching feed particles in seawater, irrespective of feed addition. <i>L. casei</i> YB-38 at a concentration of 10⁷ CFU/mL inhibited <i>V. harveyi</i> YL-10, which was initially present at 10<sup>3</sup> CFU/mL, and the inhibition lasted for 8 h. The addition of <i>L. casei</i> to the feed significantly enhanced the growth of <i>O. punctatus</i>, and modulated the gut microbiota by reducing the relative abundance of the genus <i>Vibrio</i> and increasing that of <i>Lactobacillus</i>. <i>O. punctatus</i> fed with <i>L. casei</i> YB-38 exhibited smaller fluctuations in intestinal microbiota compared to the control group, indicating the stabilizing effect of <i>L. casei</i> YB-38 on the intestinal microbiota. Furthermore, the inclusion of <i>L. casei</i> YB-38 in the feed potentially bolstered the growth metabolism of <i>O. punctatus</i>, including pathways related to protein digestion and absorption, and antigen processing.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 6","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Functional activities of Lacticaseibacillus casei in culture of Oplegnathus punctatus\",\"authors\":\"Yin Han, Duoxiang Zhao, Da Liang, Yuanyuan Sun, Wenjie Wang, Jiansong Chu, Zizhong Qi\",\"doi\":\"10.1007/s10499-025-02189-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study investigated the survival dynamics of <i>Lacticaseibacillus casei</i> YB-38 in nutrient-rich seawater and its inhibitory effect on the pathogenic bacterium <i>Vibrio harveyi</i> YL-10. <i>L. casei</i> YB-38 at 10<sup>8</sup> CFU per gram of feed was supplemented with feed in the cultivation of <i>Oplegnathus punctatus</i> (initial average weight = 5.30 ± 0.20 g), focusing on the growth characteristics for 14 days. Flow cytometry and genomics analysis were utilized to investigate the effects of <i>L. casei</i> YB-38 on the intestinal microbiota activity and immune function of <i>O. punctatus</i>. The results indicated that <i>L. casei</i> YB-38 and <i>V. harveyi</i> YL-10 demonstrate enhanced growth under simulated enriched conditions. <i>V. harveyi</i> YL-10 exhibited better growth when attaching feed particles in seawater, irrespective of feed addition. <i>L. casei</i> YB-38 at a concentration of 10⁷ CFU/mL inhibited <i>V. harveyi</i> YL-10, which was initially present at 10<sup>3</sup> CFU/mL, and the inhibition lasted for 8 h. The addition of <i>L. casei</i> to the feed significantly enhanced the growth of <i>O. punctatus</i>, and modulated the gut microbiota by reducing the relative abundance of the genus <i>Vibrio</i> and increasing that of <i>Lactobacillus</i>. <i>O. punctatus</i> fed with <i>L. casei</i> YB-38 exhibited smaller fluctuations in intestinal microbiota compared to the control group, indicating the stabilizing effect of <i>L. casei</i> YB-38 on the intestinal microbiota. Furthermore, the inclusion of <i>L. casei</i> YB-38 in the feed potentially bolstered the growth metabolism of <i>O. punctatus</i>, including pathways related to protein digestion and absorption, and antigen processing.</p></div>\",\"PeriodicalId\":8122,\"journal\":{\"name\":\"Aquaculture International\",\"volume\":\"33 6\",\"pages\":\"\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-08-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aquaculture International\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10499-025-02189-0\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture International","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s10499-025-02189-0","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}
Functional activities of Lacticaseibacillus casei in culture of Oplegnathus punctatus
This study investigated the survival dynamics of Lacticaseibacillus casei YB-38 in nutrient-rich seawater and its inhibitory effect on the pathogenic bacterium Vibrio harveyi YL-10. L. casei YB-38 at 108 CFU per gram of feed was supplemented with feed in the cultivation of Oplegnathus punctatus (initial average weight = 5.30 ± 0.20 g), focusing on the growth characteristics for 14 days. Flow cytometry and genomics analysis were utilized to investigate the effects of L. casei YB-38 on the intestinal microbiota activity and immune function of O. punctatus. The results indicated that L. casei YB-38 and V. harveyi YL-10 demonstrate enhanced growth under simulated enriched conditions. V. harveyi YL-10 exhibited better growth when attaching feed particles in seawater, irrespective of feed addition. L. casei YB-38 at a concentration of 10⁷ CFU/mL inhibited V. harveyi YL-10, which was initially present at 103 CFU/mL, and the inhibition lasted for 8 h. The addition of L. casei to the feed significantly enhanced the growth of O. punctatus, and modulated the gut microbiota by reducing the relative abundance of the genus Vibrio and increasing that of Lactobacillus. O. punctatus fed with L. casei YB-38 exhibited smaller fluctuations in intestinal microbiota compared to the control group, indicating the stabilizing effect of L. casei YB-38 on the intestinal microbiota. Furthermore, the inclusion of L. casei YB-38 in the feed potentially bolstered the growth metabolism of O. punctatus, including pathways related to protein digestion and absorption, and antigen processing.
期刊介绍：
Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture.
The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more.
This is the official Journal of the European Aquaculture Society.
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