利用啤酒糟作为唯一碳源微生物合成可生物降解聚羟基丁酸酯(PHB)聚合物及其表征

Sidrak Tesfaye Feleke, Ketema Beyecha Hundie, Yigezu Mekonnen Bayisa
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引用次数: 0

摘要

本研究采用微生物法,以啤酒废谷物为唯一碳源,合成了一种环保型聚羟基丁酸酯(PHB)生物聚合物。以枯草芽孢杆菌为辅助原料,利用废粮中回收的葡萄糖,通过细菌发酵生产聚羟基丁酸酯(PHB)。以废粮为碳源,对其进行水解制备PHB,并测定其化学成分和近似分析。废粮水解后,用本尼迪克特试验和紫外光谱法测定葡萄糖浓度。然后,采用Box-Behnken设计分析发酵时间、pH和培养温度对PHB产量的影响。实验结果表明,在温度为37℃、pH为7、发酵时间为48 hr的条件下,PHB的最佳产率为5.03 ± 0.14 g/l。利用紫外-可见分光光度计、粉末x射线衍射(XRD)、傅里叶变换红外光谱(FTIR)和热重分析(TGA)对枯草芽孢杆菌积累的PHB进行了表征,验证了聚合物的结构为PHB。同时进行了生物降解性、吸水性和水溶性试验。该研究的发现表明,利用枯草芽孢杆菌生产PHB是可能的。枯草芽孢杆菌是一种环保聚合物,利用啤酒残渣(废谷物)作为适当的碳源,可以降低生产成本,缓解材料的处理问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Utilization of brewery spent grain as an exclusive carbon source for microbial synthesis and characterization of biodegradable polyhydroxybutyrate (PHB) polymer
In the present study Microbial approach is used to synthesis an environmentally friendly Polyhydroxybutyrate (PHB) biopolymer by a utilizing brewery spent grain as an exclusive carbon source. The aim of the study was the production of Poly-hydroxy Butyrate (PHB), a bio-plastic using glucose recovered from spent grain with the aid of bacillus subtilis via bacterial fermentation. The Spent grain was utilized as a carbon source and it was hydrolyzed for PHB synthesis, also, its chemical composition and proximate analysis were determined. After the spent grain was hydrolyzed, the benedict test and UV-spectroscopy were used to determine glucose concentration. Then, the Box-Behnken designs was used to analyze the effects of fermentation duration, pH, and incubation temperature on PHB yield were assessed. According to the experimental findings, the optimum yield of PHB (5.03 ± 0.14 g/l of neat PHB) was attained at a temperature of 37 °C, pH of 7 and fermentation time of 48 hr. The Bacillus subtilis accumulated PHB was characterized by using UV-Vis spectrophotometer, Powder X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and Thermogravimetric analysis (TGA) to validate the polymer's structure as PHB. Likewise, biodegradability, water absorption and water solubility test were carried out. The study's findings demonstrated that it is possible to produce PHB using Bacillus subtilis, which is an environmentally friendly polymer using brewery’s residues (spent grain) as an appropriate carbon source to lower the cost of production and ease the material's disposal issue.
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