技术说明:通过测量雾化器中细菌悬浮液的吸光度来预测细菌气溶胶浓度:以金黄色葡萄球菌为例

IF 2.9 3区 环境科学与生态学 Q2 ENGINEERING, CHEMICAL
Dongmin Shin, Jungho Hwang
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引用次数: 0

摘要

空气中的细菌会影响室内空气质量,并对健康构成威胁。为了研制空气细菌采样器和检测装置,首先应在实验室使用雾化器进行气溶胶实验,以确定室内实际空气环境中的细菌浓度。例如,据报道,室内空气中金黄色葡萄球菌的浓度为每1立方米空气101-103菌落形成单位(cfu)。使用雾化器产生的细菌气溶胶浓度取决于雾化器内含有细菌颗粒的液体悬浮液的浓度。此外,空气中如此低浓度的细菌需要精确控制悬浮液的浓度。此外,测量生物气溶胶浓度的传统方法依赖于基于培养的技术,这种技术只能测量总微生物群落的一部分,并且具有缓慢的缺点,通常需要一到几天才能完成。本研究提出了一种预测方法,用于估计空气中的细菌浓度(cfu /m3),该方法基于使用紫外/可见光谱测量雾化器中的细菌悬浮液的吸光度。该方法包括通过制备不同浓度的细菌悬浮液,测量其吸光度,使用雾化器雾化每种悬浮液,并采样空气中细菌进行CFU计数,从而建立相关曲线。根据得到的相关曲线,只需测量某一菌悬液的吸光度,即可得到空气中细菌的CFU浓度,而无需重复进行耗时的实验。以金黄色葡萄球菌为例,空气中CFU浓度与悬浮液吸光度的R2为0.9976。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Technical note: Prediction of bacterial aerosol concentration via absorbance measurement of bacterial suspension in atomizer: Staphylococcus aureus as an example
Airborne bacteria affect indoor air quality and pose health risks. To develop airborne bacterial samplers and detection devices, aerosol experiments should first be conducted using an atomizer in the laboratory to determine the bacterial concentration in an actual indoor air environment. For example, the concentration of Staphylococcus aureus in indoor air has been reported to be 101–103 colony-forming units (CFUs) per 1 m3 of air. The bacterial aerosol concentration generated using an atomizer depends on the concentration of the liquid suspension containing bacterial particles inside the atomizer. Moreover, such low concentrations of airborne bacteria require the precise control of the suspension concentration. In addition, traditional methods of measuring bioaerosol concentrations depend on culture-based techniques, which only measure a portion of the total microbial community and have the drawback of being slow, often taking one to several days to complete. This study proposes a predictive methodology for estimating airborne bacterial concentration (CFUs/m3) based on the absorbance measurement of a bacterial suspension in an atomizer using UV/VIS spectroscopy. This methodology involves establishing a correlation curve by preparing different concentrations of bacterial suspensions, measuring their absorbances, aerosolizing each suspension using an atomizer, and sampling airborne bacteria for CFU enumeration. With the obtained correlation curve, simply measuring the absorbance of a certain bacterial suspension can yield the CFU concentration of bacteria in the air without repeatedly performing time-consuming experiments. Staphylococcus aureus was used as an example species, and the R2 between the CFU concentrations in air and the absorbances of the suspensions was 0.9976.
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来源期刊
Journal of Aerosol Science
Journal of Aerosol Science 环境科学-工程:化工
CiteScore
8.80
自引率
8.90%
发文量
127
审稿时长
35 days
期刊介绍: Founded in 1970, the Journal of Aerosol Science considers itself the prime vehicle for the publication of original work as well as reviews related to fundamental and applied aerosol research, as well as aerosol instrumentation. Its content is directed at scientists working in engineering disciplines, as well as physics, chemistry, and environmental sciences. The editors welcome submissions of papers describing recent experimental, numerical, and theoretical research related to the following topics: 1. Fundamental Aerosol Science. 2. Applied Aerosol Science. 3. Instrumentation & Measurement Methods.
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