从潮滩沉积物中分离的两个新的类胡萝卜素生成种广东变红杆菌和沉积海啸杆菌

IF 4.2 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology Pub Date : 2025-08-22 DOI:10.1016/j.syapm.2025.126649

Huan-Huan He , Ying Wang , Bing-Yu Li , Chun-Yan Lu , Shuai Li , Zhuo-Huan Zheng , Liang Zhang , Wen-Hui Lian , Xin-Tao Luo , Hai-Wei Lin , Lei Dong , Wen-Jun Li

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引用次数: 0

摘要

从珠海采集的潮滩沉积物中分离到4株需氧、革兰氏阴性、棒状和非运动细菌菌株，分别为SYSU HZ0097、SYSU HZ0136、SYSU LHT278和SYSU LHT272。基于16S rRNA基因序列的系统发育分析将4株菌株归属于红杆菌科。菌株SYSU HZ0097和SYSU HZ0136与环氧改红杆菌CGMCC 1.7731等位基因的16S rRNA基因序列相似性最高（98.6%），而菌株SYSU LHT278和SYSU LHT272与litoreella KCTC 82812等位基因的亲缘性最高（98.5%）。全基因组比较，包括平均核苷酸一致性（ANI < 85.0%）和数字DNA-DNA杂交（dDDH < 30.0%），支持它们被分配到两个不同的物种。所有菌株的主要呼吸醌为泛醌-10 (Q-10)，主要脂肪酸为C18:1 ω7c和C18:1 ω6c （> 10%）。极性脂质谱包括磷脂酰甘油、磷脂酰乙醇胺和一种未识别的糖脂。菌落呈现黄色色素沉着，并在其基因组中鉴定出类胡萝卜素生物合成相关基因（crtB, crtI）。基于系统发育、基因组学、表型和化学分类特征，提出菌株SYSU HZ0097和SYSU HZ0136为Altererythrobacter属的一个新种，命名为Altererythrobacter guangdongensis sp. 11（型菌株SYSU HZ0097 = MCCC 1K09423 = KCTC 8714 =）。菌株SYSU LHT278和SYSU LHT272代表了海啸单胞菌属的一个新种，建议将其命名为沉积海啸单胞菌sp. 11 .（型菌株SYSU LHT278 = MCCC 1K09543 = KCTC 8862 =）。

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Altererythrobacter guangdongensis sp. nov. and Tsuneonella sediminis sp. nov., two novel carotenoid-producing species isolated from tidal flat sediment

Four aerobic, Gram-stain-negative, rod-shaped, and non-motile bacterial strains, designated SYSU HZ0097ᵀ, SYSU HZ0136, SYSU LHT278ᵀ, and SYSU LHT272, were isolated from tidal flat sediment collected in Zhuhai, China. Phylogenetic analysis based on 16S rRNA gene sequences assigned all four strains to the family Erythrobacteraceae. Strains SYSU HZ0097ᵀ and SYSU HZ0136 showed the highest 16S rRNA gene sequence similarity (98.6 %) to Altererythrobacter epoxidivorans CGMCC 1.7731ᵀ, while SYSU LHT278ᵀ and SYSU LHT272 were most closely related (98.5 %) to Tsuneonella litorea KCTC 82812ᵀ. Whole-genome comparisons, including average nucleotide identity (ANI < 85.0 %) and digital DNA–DNA hybridization (dDDH <30.0 %), supported their assignment to two distinct species. All isolates contained ubiquinone-10 (Q-10) as the predominant respiratory quinone, and the major fatty acids (>10 %) were C_18:1 ω7c and C_18:1 ω6c. The polar lipid profiles included phosphatidylglycerol, phosphatidylethanolamine, and an unidentified glycolipid. The colonies exhibited yellow pigmentation, and genes associated with carotenoid biosynthesis (crtB, crtI) were identified in their genomes. Based on phylogenetic, genomic, phenotypic, and chemotaxonomic characteristics, strains SYSU HZ0097ᵀ and SYSU HZ0136 represent a new species of the genus Altererythrobacter, for which the name Altererythrobacter guangdongensis sp. nov. is proposed (type strain SYSU HZ0097ᵀ = MCCC 1K09423ᵀ = KCTC 8714ᵀ). Strains SYSU LHT278ᵀ and SYSU LHT272 represent a new species of the genus Tsuneonella, for which the name Tsuneonella sediminis sp. nov. is proposed (type strain SYSU LHT278ᵀ = MCCC 1K09543ᵀ = KCTC 8862ᵀ).

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来源期刊

Systematic and applied microbiology 生物-生物工程与应用微生物

CiteScore

7.50

自引率

5.90%

发文量

审稿时长

22 days

期刊介绍： Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: