从汉族男性新生儿脐带血中提取临床级hla纯合诱导多能干细胞UNIONi001-A

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-08-23 DOI:10.1016/j.scr.2025.103818

Xiaohui Shi , Qianping Zhang , Lijuan Cui , Ying Jia , Jinmei Zhang , Li Zhang , Ruizhen Hou , Wenling Yang , Wei Du , Yu Zhang

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引用次数: 0

摘要

临床级iPSC系UNIONi001-A来源于健康汉族男性新生儿的hla纯合子脐带血单个核细胞（CBMCs）。使用含OCT3/4、SOX2、KLF4、c-MYC和BCL-XL的episal质粒对细胞进行重编程。iPSCs表现出典型的多能干细胞形态，表达多能标记物，保持正常核型，可分化为三种胚层，适合细胞治疗研究和临床应用。

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Derivation of clinical-grade HLA-homozygous induced pluripotent stem cell line UNIONi001-A from umbilical cord blood of a Han Chinese male neonate

The clinical-grade iPSC line UNIONi001-A is derived from HLA-homozygous (HLAh) umbilical cord blood mononuclear cells (CBMCs) of a healthy Han Chinese male neonate. Cells were reprogrammed using episomal plasmids with OCT3/4, SOX2, KLF4, c-MYC and BCL-XL. The iPSCs exhibited typical pluripotent stem cell morphology, expressed pluripotency markers, maintained a normal karyotype, and could differentiate into three germ layers, making them suitable for cell therapy research and clinical use.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.