加拿大安大略省西北部淡水微藻的分离、鉴定及培养条件优化

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-08-21 DOI:10.1016/j.algal.2025.104264

Janak Raj Khatiwada , Ou Li , Chonlong Chio , Rishnika Boteju , Dhanushya Thilagam Jeyakumar , Chao Liu , Sarita Shrestha , Wensheng Qin

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引用次数: 0

摘要

原生淡水微藻因其独特的特性和环境适应性在生物燃料生产中发挥着至关重要的作用。本研究调查了加拿大安大略省西北部不同淡水生态系统中微藻物种的多样性，并分离出具有较高生物量和产脂能力的微藻物种。在75株微藻中，通过分子和形态学方法鉴定出5株产脂率和生长速度较高的微藻菌株。在4个不同的光周期中，16L:8D光/暗循环能显著提高小球藻、普通小球藻和衣藻的生物量和脂质。结果表明，安大略北部地区微藻物种多样性丰富，具有较高生物量生产力的脂质积累菌株具有发展前景。本研究强调了利用本地微藻菌株作为可再生脂质和生物质来源用于生物燃料和生物分子生产的潜力。

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Isolation, identification and optimization of culture condition of freshwater microalgae in Northwestern Ontario, Canada for sustainable lipid and biomass production

Native freshwater microalgae play a crucial role in biofuel production due to their unique characteristics and environmental suitability. This study investigated the diversity of microalgae species in various freshwater ecosystems across Northwestern Ontario, Canada and isolated microalgae species with higher biomass and lipid production capacity. Among the 75 strains, a total of five microalgae strains with higher lipid production and growth were identified using molecular and morphological approaches. Among four different photoperiods, 16L:8D light/dark cycle produced significantly higher biomass and lipids for Chlorella sorokiniana, Chlorella vulgaris, and Chlamydomonas sp. The results indicated a rich diversity of microalgae species from the region of Northern Ontario, with promising lipid accumulation isolates with high biomass productivity. This study highlighted the potential for utilizing native microalgae strains as the source of renewable lipids and biomass for biofuel and biomolecule production.

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来源期刊

Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

9.40

自引率

7.80%

发文量

332

期刊介绍： Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment