抗菌聚合物中的糖-糖-凝集素结合对阳离子糖共聚物膜相互作用的影响

IF 4.4 3区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Alain M. Bapolisi, Steffen Tank, Anne-Catherine Lehnen, Antje Stindt, Rujuta Athavale, Jan A. M. Kurki, Sany Chea, Sophia Rosencrantz, Ruben R. Rosencrantz, Katja M. Arndt, Matthias Hartlieb
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引用次数: 0

摘要

膜活性抗微生物聚合物(APs)是治疗传染病的有希望的替代品,因为它们不容易产生抗微生物药物耐药性。然而,其非特异性细胞毒性限制了其临床应用。改善细胞相容性的一个有趣策略是将糖单位掺入ap中。这种方法利用了细菌表面的糖和蛋白质受体(如凝集素)之间的特定相互作用,从而有可能区分不同类型的细菌。然而,关于添加糖如何影响阳离子ap的选择性和与凝集素的相互作用的研究有限。本文通过黄原酸负载的光引发可逆加成-断裂链转移(XPI-RAFT)聚合合成了丙烯酰胺基阳离子共聚物库。通过糖共聚物(基于焦糖、葡萄糖、甘露糖和多羟基丙烯酰胺)的链延伸或简单的还原性胺化(乳糖和糖二糖),将各种糖添加到阳离子支架中。各种分析技术被用来探测(糖)聚合物与细菌、膜模型和凝集素的相互作用。一般来说,(糖)多羟基掺入证明降低阳离子APs的疏水性,从而增强血液相容性,同时保持抗菌活性。虽然糖蛋白特异性识别可能有助于选择性,但结果表明,静电相互作用主要驱动阳离子糖共聚物与细菌膜的结合效率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Sugars in Antimicrobial Polymers – The Impact of Sugar-Lectin Binding in Membrane Interaction of Cationic Glycopolymers

Sugars in Antimicrobial Polymers – The Impact of Sugar-Lectin Binding in Membrane Interaction of Cationic Glycopolymers

Sugars in Antimicrobial Polymers – The Impact of Sugar-Lectin Binding in Membrane Interaction of Cationic Glycopolymers

Sugars in Antimicrobial Polymers – The Impact of Sugar-Lectin Binding in Membrane Interaction of Cationic Glycopolymers

Sugars in Antimicrobial Polymers – The Impact of Sugar-Lectin Binding in Membrane Interaction of Cationic Glycopolymers

Membrane-active antimicrobial polymers (APs) are promising alternatives for treating infectious diseases as they are less susceptible to antimicrobial resistance development. However, their non-specific cytotoxicity limits their clinical application. One interesting strategy to improve cytocompatibility is the incorporation of sugar units into APs. This approach takes advantage of specific interactions between sugars and protein receptors, such as lectins, on the surface of bacteria, potentially allowing for discrimination between different bacterial types. However, there is limited research on how sugar addition to cationic APs affects their selectivity and interactions with lectins. Herein, a library of acrylamide-based cationic glycopolymers is synthesized by a xanthate-supported photo-initiated Reversible Additon-Fragmentation chain Trasnfer (XPI-RAFT) polymerization. Diverse sugars are added to this cationic scaffold by either chain extension with glycomonomers (based on fucose, glucose, mannose, and polyhydroxy acrylamide) or simply by reductive amination (lactose and melibiose). Various analytical techniques are used to probe the interaction of (glyco)polymers with bacteria, membrane models, and lectins. In general, (glyco)polyhydroxy incorporation proves to reduce the hydrophobicity of the cationic APs and consequently enhances hemocompatibility while maintaining the antimicrobial activity. Although sugar-protein-specific recognition may contribute to selectivity, the results demonstrate that electrostatic interaction predominantly drives the binding efficacy of cationic glycopolymers to bacterial membranes.

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来源期刊
Advanced Materials Interfaces
Advanced Materials Interfaces CHEMISTRY, MULTIDISCIPLINARY-MATERIALS SCIENCE, MULTIDISCIPLINARY
CiteScore
8.40
自引率
5.60%
发文量
1174
审稿时长
1.3 months
期刊介绍: Advanced Materials Interfaces publishes top-level research on interface technologies and effects. Considering any interface formed between solids, liquids, and gases, the journal ensures an interdisciplinary blend of physics, chemistry, materials science, and life sciences. Advanced Materials Interfaces was launched in 2014 and received an Impact Factor of 4.834 in 2018. The scope of Advanced Materials Interfaces is dedicated to interfaces and surfaces that play an essential role in virtually all materials and devices. Physics, chemistry, materials science and life sciences blend to encourage new, cross-pollinating ideas, which will drive forward our understanding of the processes at the interface. Advanced Materials Interfaces covers all topics in interface-related research: Oil / water separation, Applications of nanostructured materials, 2D materials and heterostructures, Surfaces and interfaces in organic electronic devices, Catalysis and membranes, Self-assembly and nanopatterned surfaces, Composite and coating materials, Biointerfaces for technical and medical applications. Advanced Materials Interfaces provides a forum for topics on surface and interface science with a wide choice of formats: Reviews, Full Papers, and Communications, as well as Progress Reports and Research News.
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