门系统分流犬血清hepcidin异构体水平升高

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-08-20 DOI:10.1016/j.rvsc.2025.105862

Zsuzsanna Vizi , Katalin Lányi , David Hotchkiss , Ágnes Andrea Sterczer

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引用次数: 0

摘要

小细胞增多和铁固存是门静脉系统分流（PSS）犬的常见异常。hepcidin——调节铁体内平衡的主要激素——在这种情况下的作用尚不清楚。我们的研究是为了确定已知的hepcidin同分异构体在犬门静脉分流患者的血清浓度，并与健康对照组进行比较。对10只诊断为PSS的犬和18只健康对照犬进行常规血液学和生化检查。采用液相色谱-串联质谱（LC/MS-MS）法测定血清中hepcidin-α和hepcidin-β异构体的浓度。在这项研究中，与健康犬相比，PSS组血清hepcidin异构体浓度均显著（p < 0.05）升高（hepcidin-α: 46.1 vs. 23.1 ng·mL - 1, p = 0.014; hepcidin-β: 76.6 vs. 48.4 ng·mL - 1, p = 0.027）。在PSS犬中，铁与hepcidin异构体浓度呈显著负相关（hepcidin-α: rho: - 0.81, p = 0.007, hepcidin-β rho: - 0.84, p = 0.0004）。我们的研究结果表明，血清hepcidin浓度升高在PSS患者的小细胞增多和铁固存中起重要作用；然而，需要在大队列患者中进行进一步的研究。血清hepcidin可作为pss相关性贫血患者的潜在生物标志物。

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Elevated levels of serum hepcidin isomers in dogs with portosystemic shunt

Microcytosis and iron sequestration are commonly observed abnormalities in dogs with portosystemic shunt (PSS). The role of hepcidin – the main hormone regulating iron homeostasis – in this condition is still unclear. Our study is intended to determine the serum concentrations of known hepcidin isomers in canine patients with portosystemic shunt, compared to a healthy control group.

Routine haematological and biochemical examinations were performed for 10 dogs diagnosed with PSS and for 18 healthy controls. And the serum concentrations of hepcidin-α and hepcidin-β isomers were determined by liquid chromatography-tandem mass spectrometry (LC/MS-MS) method.

In this study, both serum hepcidin isomer concentrations were significantly (p < 0,05) higher in the PSS group compared to healthy dogs (hepcidin-α: 46.1 vs. 23.1 ng·mL⁻¹, p = 0.014, hepcidin-β: 76.6 vs 48.4 ng·mL⁻¹, p = 0.027). In dogs with PSS, there was a significant negative correlation between iron and hepcidin isomer concentrations (hepcidin-α: rho: −0.81, p = 0.007, hepcidin-β rho: −0.84, p = 0.0004).

Our results suggest that elevated serum hepcidin concentration plays an important role in the development of microcytosis and iron sequestration observed in patients with PSS; however, further studies needed in large cohort of patients. Serum hepcidin could be used as a potential biomarker in patients with PSS-associated anaemia.

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.