表达dCas9-TET1融合蛋白的人胚胎干细胞系用于表观遗传编辑

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-08-20 DOI:10.1016/j.scr.2025.103811

Ji-Woo Kim, Seongyea Jo, Eun-Hye Kang, Ji Hyeon Ryu, Haneul Noh, Han-Jin Park, Hyemin Kim

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引用次数: 0

摘要

基于crispr的表观基因组编辑系统可以诱导位点特异性转录激活或抑制靶基因。10 - 11易位甲基胞嘧啶双加氧酶1 （TET1）是参与基因调控区CpG二核苷酸胞嘧啶去甲基化的转录激活效应因子。在这项研究中，我们生成了一种人类胚胎干细胞系，它通过慢病毒转导稳定地表达催化死亡的Cas9 （dCas9）融合到TET1的催化结构域。该细胞系可与单导rna结合用于位点特异性转录激活，并可作为干细胞和类器官模型中表观遗传调控的有价值工具。

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Generation of human embryonic stem cell line expressing dCas9-TET1 fusion protein for epigenetic editing

CRISPR-based epigenome editing systems can induce site-specific transcriptional activation or repression of target genes. Ten-eleven translocation methylcytosine dioxygenase 1 (TET1) is a transcriptional activation effector involved in the cytosine demethylation of CpG dinucleotides in gene regulatory regions. In this study, we generated a human embryonic stem cell line that stably expresses catalytically dead Cas9 (dCas9) fused to the catalytic domain of TET1 via lentiviral transduction. This cell line can be used for locus-specific transcriptional activation in combination with single guide RNAs and serves as a valuable tool for epigenetic regulation in stem cell and organoid models.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.