改进的数字无透镜全息显微镜重建的对数动态范围

IF 3.7 2区 工程技术 Q2 OPTICS
Maria J. Lopera , René Restrepo , Yunfeng Nie , Heidi Ottevaere , Carlos Trujillo
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引用次数: 0

摘要

在次优照明条件下,DLHM通常难以在整个传感器区域捕获有用的全息信息。当使用相对较大的、空间扩展的点源(≥5µm)或非常短的工作距离(< 10 mm)时尤其如此。在这种情况下,记录的全息图通常只在一个小的、明亮的中心区域显示有用的细节。由于这些情况在实际应用中很常见,因此数字预处理变得必不可少。它可以大大提高重建波场的质量。这项工作提出了一种无硬件的单帧预处理方法,称为对数动态范围(LogDR)。它将记录的辐照度重新分配到对数尺度上,然后应用局部色调映射。这种方法在不引入伪影的情况下使DLHM中的特征高斯照明包络变平。对USAF-1951目标的8张受限照度全息图的初步验证表明,提出的LogDR方法将有效视场增加了约13%,将最小可分辨特征从3.10µm提高到1.95µm,同时将背景相位噪声降低了4倍。与其他方法相比,LogDR在视场、横向分辨率和相位对比度之间提供了最好的平衡。口腔黏液涂片的生物学演示进一步揭示了外周蛋白聚集体和亚细胞细节,这些细节仍然隐藏在传统的重建中。由于LogDR无需额外的曝光、光学或机器学习模型,因此它为便携式或医疗点DLHM系统提供了可立即部署的增强功能,可以在较差的照明条件下实现更广泛的上下文成像和更精细的结构分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Logarithmic dynamic range for improved digital lensless holographic microscopy reconstructions
Under sub-optimal illumination conditions, DLHM often struggles to capture useful holographic information across the full sensor area. This is especially true when using relatively large, spatially extended point sources (≥ 5 µm) or very short working distances (< 10 mm). In such cases, the recorded holograms typically show useful detail only in a small, brightly illuminated central region. Since these conditions are common in practical applications, numerical preprocessing becomes essential. It can substantially improve the quality of the reconstructed wave-fields. This work proposes a hardware-free, single-frame preprocessing method called Logarithmic Dynamic Range (LogDR). It redistributes the recorded irradiance onto a logarithmic scale and then applies local tone mapping. This approach flattens the characteristic Gaussian illumination envelope in DLHM without introducing artifacts. Initial validation with eight illumination-limited holograms of a USAF-1951 target shows that the proposed LogDR method increases the effective field of view by about 13 % and improves the smallest resolvable feature from 3.10 µm to 1.95 µm while reducing background phase noise by four-fold. Compared with other methods, LogDR delivers the best trade-off between field of view, lateral resolution and phase contrast. A biological demonstration on a buccal-mucus smear further reveals peripheral protein aggregates and sub-cellular details that remain hidden in conventional reconstructions. Because LogDR operates without additional exposures, optics or machine-learning models, it offers an immediately deployable enhancement for portable or point-of-care DLHM systems, enabling wider contextual imaging and finer structural analysis under compromised illumination conditions.
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来源期刊
Optics and Lasers in Engineering
Optics and Lasers in Engineering 工程技术-光学
CiteScore
8.90
自引率
8.70%
发文量
384
审稿时长
42 days
期刊介绍: Optics and Lasers in Engineering aims at providing an international forum for the interchange of information on the development of optical techniques and laser technology in engineering. Emphasis is placed on contributions targeted at the practical use of methods and devices, the development and enhancement of solutions and new theoretical concepts for experimental methods. Optics and Lasers in Engineering reflects the main areas in which optical methods are being used and developed for an engineering environment. Manuscripts should offer clear evidence of novelty and significance. Papers focusing on parameter optimization or computational issues are not suitable. Similarly, papers focussed on an application rather than the optical method fall outside the journal''s scope. The scope of the journal is defined to include the following: -Optical Metrology- Optical Methods for 3D visualization and virtual engineering- Optical Techniques for Microsystems- Imaging, Microscopy and Adaptive Optics- Computational Imaging- Laser methods in manufacturing- Integrated optical and photonic sensors- Optics and Photonics in Life Science- Hyperspectral and spectroscopic methods- Infrared and Terahertz techniques
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