Maria J. Lopera , René Restrepo , Yunfeng Nie , Heidi Ottevaere , Carlos Trujillo
{"title":"改进的数字无透镜全息显微镜重建的对数动态范围","authors":"Maria J. Lopera , René Restrepo , Yunfeng Nie , Heidi Ottevaere , Carlos Trujillo","doi":"10.1016/j.optlaseng.2025.109292","DOIUrl":null,"url":null,"abstract":"<div><div>Under sub-optimal illumination conditions, DLHM often struggles to capture useful holographic information across the full sensor area. This is especially true when using relatively large, spatially extended point sources (≥ 5 µm) or very short working distances (< 10 mm). In such cases, the recorded holograms typically show useful detail only in a small, brightly illuminated central region. Since these conditions are common in practical applications, numerical preprocessing becomes essential. It can substantially improve the quality of the reconstructed wave-fields. This work proposes a hardware-free, single-frame preprocessing method called Logarithmic Dynamic Range (<em>LogDR</em>). It redistributes the recorded irradiance onto a logarithmic scale and then applies local tone mapping. This approach flattens the characteristic Gaussian illumination envelope in DLHM without introducing artifacts. Initial validation with eight illumination-limited holograms of a USAF-1951 target shows that the proposed <em>LogDR</em> method increases the effective field of view by about 13 % and improves the smallest resolvable feature from 3.10 µm to 1.95 µm while reducing background phase noise by four-fold. Compared with other methods, <em>LogDR</em> delivers the best trade-off between field of view, lateral resolution and phase contrast. A biological demonstration on a buccal-mucus smear further reveals peripheral protein aggregates and sub-cellular details that remain hidden in conventional reconstructions. Because <em>LogDR</em> operates without additional exposures, optics or machine-learning models, it offers an immediately deployable enhancement for portable or point-of-care DLHM systems, enabling wider contextual imaging and finer structural analysis under compromised illumination conditions.</div></div>","PeriodicalId":49719,"journal":{"name":"Optics and Lasers in Engineering","volume":"195 ","pages":"Article 109292"},"PeriodicalIF":3.7000,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Logarithmic dynamic range for improved digital lensless holographic microscopy reconstructions\",\"authors\":\"Maria J. Lopera , René Restrepo , Yunfeng Nie , Heidi Ottevaere , Carlos Trujillo\",\"doi\":\"10.1016/j.optlaseng.2025.109292\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Under sub-optimal illumination conditions, DLHM often struggles to capture useful holographic information across the full sensor area. This is especially true when using relatively large, spatially extended point sources (≥ 5 µm) or very short working distances (< 10 mm). In such cases, the recorded holograms typically show useful detail only in a small, brightly illuminated central region. Since these conditions are common in practical applications, numerical preprocessing becomes essential. It can substantially improve the quality of the reconstructed wave-fields. This work proposes a hardware-free, single-frame preprocessing method called Logarithmic Dynamic Range (<em>LogDR</em>). It redistributes the recorded irradiance onto a logarithmic scale and then applies local tone mapping. This approach flattens the characteristic Gaussian illumination envelope in DLHM without introducing artifacts. Initial validation with eight illumination-limited holograms of a USAF-1951 target shows that the proposed <em>LogDR</em> method increases the effective field of view by about 13 % and improves the smallest resolvable feature from 3.10 µm to 1.95 µm while reducing background phase noise by four-fold. Compared with other methods, <em>LogDR</em> delivers the best trade-off between field of view, lateral resolution and phase contrast. A biological demonstration on a buccal-mucus smear further reveals peripheral protein aggregates and sub-cellular details that remain hidden in conventional reconstructions. Because <em>LogDR</em> operates without additional exposures, optics or machine-learning models, it offers an immediately deployable enhancement for portable or point-of-care DLHM systems, enabling wider contextual imaging and finer structural analysis under compromised illumination conditions.</div></div>\",\"PeriodicalId\":49719,\"journal\":{\"name\":\"Optics and Lasers in Engineering\",\"volume\":\"195 \",\"pages\":\"Article 109292\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-08-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Optics and Lasers in Engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0143816625004774\",\"RegionNum\":2,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"OPTICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Optics and Lasers in Engineering","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0143816625004774","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OPTICS","Score":null,"Total":0}
Logarithmic dynamic range for improved digital lensless holographic microscopy reconstructions
Under sub-optimal illumination conditions, DLHM often struggles to capture useful holographic information across the full sensor area. This is especially true when using relatively large, spatially extended point sources (≥ 5 µm) or very short working distances (< 10 mm). In such cases, the recorded holograms typically show useful detail only in a small, brightly illuminated central region. Since these conditions are common in practical applications, numerical preprocessing becomes essential. It can substantially improve the quality of the reconstructed wave-fields. This work proposes a hardware-free, single-frame preprocessing method called Logarithmic Dynamic Range (LogDR). It redistributes the recorded irradiance onto a logarithmic scale and then applies local tone mapping. This approach flattens the characteristic Gaussian illumination envelope in DLHM without introducing artifacts. Initial validation with eight illumination-limited holograms of a USAF-1951 target shows that the proposed LogDR method increases the effective field of view by about 13 % and improves the smallest resolvable feature from 3.10 µm to 1.95 µm while reducing background phase noise by four-fold. Compared with other methods, LogDR delivers the best trade-off between field of view, lateral resolution and phase contrast. A biological demonstration on a buccal-mucus smear further reveals peripheral protein aggregates and sub-cellular details that remain hidden in conventional reconstructions. Because LogDR operates without additional exposures, optics or machine-learning models, it offers an immediately deployable enhancement for portable or point-of-care DLHM systems, enabling wider contextual imaging and finer structural analysis under compromised illumination conditions.
期刊介绍:
Optics and Lasers in Engineering aims at providing an international forum for the interchange of information on the development of optical techniques and laser technology in engineering. Emphasis is placed on contributions targeted at the practical use of methods and devices, the development and enhancement of solutions and new theoretical concepts for experimental methods.
Optics and Lasers in Engineering reflects the main areas in which optical methods are being used and developed for an engineering environment. Manuscripts should offer clear evidence of novelty and significance. Papers focusing on parameter optimization or computational issues are not suitable. Similarly, papers focussed on an application rather than the optical method fall outside the journal''s scope. The scope of the journal is defined to include the following:
-Optical Metrology-
Optical Methods for 3D visualization and virtual engineering-
Optical Techniques for Microsystems-
Imaging, Microscopy and Adaptive Optics-
Computational Imaging-
Laser methods in manufacturing-
Integrated optical and photonic sensors-
Optics and Photonics in Life Science-
Hyperspectral and spectroscopic methods-
Infrared and Terahertz techniques