靶向尿谱作为膳食中水果和蔬菜类黄酮摄入量的生物标志物及其与膳食评估时间的关系

IF 3.2 Q2 NUTRITION & DIETETICS

Current Developments in Nutrition Pub Date : 2025-07-31 DOI:10.1016/j.cdnut.2025.107520

Emily B Hill , Anna Pashkova , Elizabeth M Grainger , Kristen M Roberts , Chureeporn Chitchumroonchokchai , Ashlea C Braun , Robin A Ralston , Ken M Riedl , Colleen K Spees , Steven K Clinton

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引用次数: 0

摘要

黄酮类化合物是一类植物（多）酚，据推测，饮食摄入黄酮类化合物可以调节与水果和蔬菜（FV）摄入有关的各种有益健康结果。被吸收的黄酮类化合物在尿液中代谢和排泄，可能作为FV摄入和宿主代谢的生物标志物，但尿液生物标志物与摄入特定时间的关系尚不清楚。目的研究尿中6种黄酮类化合物的靶组特征，并确定其与成人膳食黄酮类化合物摄入量的关系。方法采用苯酚- explorer数据库，通过30 d食物频率问卷和3-d饮食记录估算17名成年人的膳食类黄酮摄入量。采集24小时尿液，采用高压液相色谱-二极管阵列检测（DAD）定量测定尿中6种黄酮类化合物（槲皮素、根皮素、柚皮素、橙皮素、山奈酚和异鼠李素）。结果与1 d或3 d饮食记录的估计摄入量相比，尿总黄酮与2 d饮食记录（包括前一天和24小时尿液收集当天）估计的总FV（不包括土豆）摄入量（rs = 0.53, P = 0.028）和总FV类黄酮摄入量（rs = 0.60, P = 0.011）相关性最强。通过30天的食物频率问卷调查，尿中黄酮类化合物的浓度与估计的FV摄入量之间没有显著相关性（rs = 0.36, P = 0.16）。结论：24小时尿液收集中有针对性的黄酮类化合物浓度可作为尿液收集完成前2天饮食暴露的生物标志物，但不能作为更远距离饮食暴露的生物标志物。该试验在clinicaltrials.gov注册为NCT03489213。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Targeted Urinary Profiling as a Biomarker of Dietary Flavonoid Intake from Fruits and Vegetables and its Relationship to the Timing of Dietary Assessment

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Targeted Urinary Profiling as a Biomarker of Dietary Flavonoid Intake from Fruits and Vegetables and its Relationship to the Timing of Dietary Assessment

Background

Dietary intake of flavonoids, a class of plant (poly)phenols, is hypothesized to mediate a variety of beneficial health outcomes linked to fruits and vegetables (FV) intake. Absorbed flavonoids are metabolized and excreted in urine and may serve as biomarkers of FV intake and host metabolism, but defining the relationship of urinary biomarkers to the specific time of intake is unclear.

Objectives

To characterize a targeted panel of 6 urinary flavonoids and determine the relationship to estimated dietary flavonoid intake over time in adults.

Methods

Dietary flavonoid intake was estimated from both a 30-d food frequency questionnaire and 3-d diet records using the Phenol-Explorer database in 17 adults. Twenty-four-hour urine was collected, and a targeted panel of 6 urinary flavonoids (quercetin, phloretin, naringenin, hesperetin, kaempferol, and isorhamnetin) was quantified using high-pressure liquid chromatography-diode array detection (DAD).

Results

Compared to estimated intakes from a 1 d or 3 d diet record, total urinary flavonoids were most strongly associated with total FV (excluding potatoes) (r_s = 0.53, P = 0.028) and total FV flavonoid (r_s = 0.60, P = 0.011) intakes estimated by a 2 d diet record (including the day before and the day of the 24-h urine collection). There were no significant correlations between urinary concentrations of flavonoids and estimated FV intake using a 30-d food frequency questionnaire (r_s = 0.36, P = 0.16).

Conclusions

Targeted flavonoid concentrations in a 24-h urine collection may serve as a biomarker for estimating dietary exposures over 2 d prior to completion of the urine collection, but not more distant dietary exposures.

This trial was registered at clinicaltrials.gov as NCT03489213.

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