生成两个Betacellulin CRISPR-Cas9敲除的hiPSC系，研究精神分裂症中受影响的EGF系统范式

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-08-19 DOI:10.1016/j.scr.2025.103808

Agustin Cota-Coronado , Murray Manning , Dong-Hyung Kim , Joohyung Lee , Andrew Gibbons , Joseph Rosenbluh , Rachel A. Hill , Suresh Sundram

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引用次数: 0

摘要

表皮生长因子（EGF）家族的几个成员与精神分裂症的生物学有关（Ketharanathan等人，2024）。egf相关配体Betacellulin （BTC）在神经干细胞的增殖和分化中起重要作用，本课题组发现精神分裂症患者的BTC水平显著降低。然而，受影响的BTC及其参与神经规范和神经发育的相互作用仍然难以捉摸。我们通过CRISPR/ cas9介导的修饰，从现有的hiPSC细胞系中生成了Knockout (KO) - BTC克隆。此外，我们通过基因分型/测序、FACS和Western Blot验证了BTC-KO。最后，我们在体外证明了三岁分化的潜力。

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Generation of two Betacellulin CRISPR-Cas9 knockout hiPSC lines to study the affected EGF system paradigm in schizophrenia

Several members of the epidermal growth factor (EGF) family have been implicated in the biology of schizophrenia (Ketharanathan et al., 2024). The EGF-related ligand, Betacellulin (BTC), plays an important role in the proliferation and differentiation of neural stem cells and our group found markedly reduced BTC levels in patients with schizophrenia. Nevertheless, the interplay of affected BTC and its participation in neural specification and neurodevelopment remains elusive. We generated Knockout (KO) − BTC clones from an existing hiPSC line through CRISPR/Cas9-mediated modification. Furthermore, we validated BTC-KO through genotyping/sequencing, FACS and Western Blot. Finally, we demonstrated trilineage differentiation potential in vitro.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.