Aysel Gurbanova, Anne Stoverink, Imke M. B. van Wandeloo, Annemiek Nap, Nicole M. de Roos, Marien I. de Jonge, Janneke S. Hoogstad - van Evert, Renate G. van der Molen
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引用次数: 0
摘要
月经流出物（ME）被广泛用于研究免疫相关生殖疾病。然而，由于对避孕药具对子宫内膜免疫细胞组成的影响了解有限，使用避孕药具的妇女往往被排除在此类研究之外。这一知识差距限制了我们将使用避孕药具的妇女纳入科学调查并解释与避孕药具使用相关的潜在免疫变异的能力。因此，我们的目的是研究避孕是否与子宫免疫系统的差异有关。研究方法：从使用联合口服避孕药（COC, n = 5）、铜质宫内节育器（IUD, n = 3）或未使用避孕药（n = 5）的妇女中收集ME。从ME中分离免疫细胞，用流式细胞术进行分析。使用非配对t检验分析数据，然后使用错误发现率（FDR）方法进行多重检验校正。结果服用避孕药的妇女与未服用避孕药的妇女相比，免疫调节性CD56brightCD16−子宫内膜自然杀伤细胞（eNK）的频率显著降低（30.1%±5.99和66.0%±10.6;p = 0.001）。此外，与COC使用者和对照组相比，使用铜宫内节育器的女性Ki-67+CD8+ T细胞增殖频率升高（19.8%±4.3,2.69%±1.23和3.77%±4.4）。这项初步研究强调了在研究免疫相关生殖问题时考虑使用避孕药具的重要性。
Menstrual Effluent Derived Immune Cell Composition Is Distinct in Women Using Contraceptives: A Pilot Study
Problem
Menstrual effluent (ME) is widely used to study immune-related reproductive disorders. However, women using contraceptives are often excluded from such studies due to limited knowledge of their impact on the endometrial immune cell composition. This gap in knowledge restricts our ability to include women using contraceptives in the scientific investigations and account for potential immunological variations associated with contraceptive use. Therefore, we aim to investigate whether contraceptive use is associated with differences in the uterine immune system.
Method of Study
ME was collected from women using combined oral contraceptives (COC, n = 5), a copper intrauterine device (IUD, n = 3), or no contraceptives (n = 5). Immune cells were isolated from ME and analyzed using flow-cytometry. Data were analyzed using an unpaired t-test, followed by multiple testing correction using the false discovery rate (FDR) method.
Results
Women using COCs exhibited a marked reduction in the frequency of the immunoregulatory CD56brightCD16− endometrial Natural Killer (eNK) cells compared to women using no contraceptives (30.1% ± 5.99 and 66.0% ± 10.6; p = 0.001). Additionally, women using copper IUD showed elevated frequencies of proliferating Ki-67+CD8+ T cells compared to COC users and controls (19.8% ± 4.3, 2.69% ± 1.23, and 3.77% ± 4.4).
Conclusions
This pilot study highlights the importance of considering contraceptive use when studying immune-related reproductive problems.
期刊介绍：
The American Journal of Reproductive Immunology is an international journal devoted to the presentation of current information in all areas relating to Reproductive Immunology. The journal is directed toward both the basic scientist and the clinician, covering the whole process of reproduction as affected by immunological processes. The journal covers a variety of subspecialty topics, including fertility immunology, pregnancy immunology, immunogenetics, mucosal immunology, immunocontraception, endometriosis, abortion, tumor immunology of the reproductive tract, autoantibodies, infectious disease of the reproductive tract, and technical news.
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