光学激光层析成像技术研究藻类细胞亚细胞结构的分布

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology

Biophysics Pub Date : 2025-08-22 DOI:10.1134/S0006350925700307

A. A. Samoilenko, G. G. Levin, A. A. Volgusheva, A. P. Kazakov, G. V. Maksimov

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引用次数: 0

摘要

利用光学激光层析成像技术，证明了记录和分析真核藻类亚细胞细胞器三维分布的可能性。该方法允许人们分析亚细胞器（细胞核和叶绿体）在正常情况下和在其功能状态的修饰剂的影响下在一个细胞的连续切片中的重新分布。根据作者的说法，该方法不仅可以研究细胞内结构（核、类囊体、线粒体等）分布的动力学，还可以研究细胞功能过程中单个结构的变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A Study of the Distribution of Subcellular Structures in Algae Cells Using Optical Laser Tomography

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A Study of the Distribution of Subcellular Structures in Algae Cells Using Optical Laser Tomography

Using the developed method of optical laser tomography, the possibility of recording and analyzing the three-dimensional distribution of subcellular organelles in eukaryotic algae (C. rainhardtii) was proved. The method allows one to analyze the redistribution of subcellular organelles (the nucleus and chloroplast) in consecutive slices of one cell in the norm and under the influence of modifiers of its functional state. According to the authors, the proposed approach will allow one not only to study the dynamics of the distribution of intracellular structures (nucleus, thylakoids, mitochondria, etc.), but also changes of a single structure during cell functioning.

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.