通过EMS诱变诱导Davana （Artemisia pallens Bess.）的遗传多样性，并利用ISSR标记支持其农艺和化学性状

IF 1.6 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2025-08-19 DOI:10.1016/j.plgene.2025.100543

Arun J. Ganiger , Channayya Hiremath , K. Madhusudan , Manoj kumar Chandrasekaran , V.S. Pragadheesh

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引用次数: 0

摘要

Davana （Artemisia pallens Bess.）是一种一年生芳香草本植物，属于菊科。本研究旨在鉴定具有高davdavone含量的davdavone优质高精油突变体。为了确定EMS的LD50值，用不同浓度的化学诱变剂甲烷磺酸乙酯（EMS）处理本地达瓦纳品种“班加罗尔”的种子，分别为0.10%、0.2%、0.3%、0.40%和0.50%，处理16 h。通过probit分析，LD50值为0.40% （16 h）。在M1代中，对3000株达瓦纳草木进行了评价，并转发到M2上。在1500 M2植株目视观察的基础上，选择250个突变体，对其挥发油和达凡酮含量进行评价。在M2代中，所评价的数量性状和所评价的化学成分与对照相比，具有较高的平均变异和中高的表型变异系数。精油产量与单株花数、花头直径、鲜草产量性状呈极显著正相关。根据M2群体的对照优势和Mean + 1 SD，分别鉴定出22个和14个挥发油产量和davdavone含量的突变体。利用ISSR标记对16株精油高产植物M2和2个对照品种进行分子多样性分析，发现其多态性平均为79%，其中引物UBC-845多态性信息含量最高。本研究鉴定出DM2158（挥发油含量- 0.5%）和DM2103（达瓦酮含量- 70.92%）两个优良突变体，表明突变选育是培育高挥发油和达瓦酮含量达瓦纳新品种的有效策略。

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Induced genetic diversity through EMS mutagenesis in Davana (Artemisia pallens Bess.) and use of ISSR markers to underpin agronomical and chemical traits

Davana (Artemisia pallens Bess.) is an annual aromatic herb that belongs to the Asteraceae family. The current research was attempted to identify elite high essential oil yielding davana mutants with high davanone content. To determine the LD₅₀ value of EMS, seeds of the local davana cultivar “Bangalore” were treated with the chemical mutagen ethyl methane sulphonate (EMS) at varying concentrations of 0.10 %, 0.2 %, 0.3 %, 0.40 %, and 0.50 % for 16 h. The LD₅₀ value was (0.40 % for 16 h) determined by probit analysis. In the M₁ generation, 3000 davana plants were evaluated and forwarded to M₂. Based on visual observation of 1500 M₂ plants, 250 mutants were chosen, and their essential oil and davanone content was assessed. In the M₂ generation, the quantitative traits evaluated and chemical constituents assessed had higher mean and moderate to high phenotypic co-efficient of variation, in comparison to the control. Essential oil yield had a strong and significantly positive correlation to the number of flowers per plant, flower head diameter, and fresh herb yield traits. Based on superiority over control and Mean + 1 SD of M₂ population, 22 and 14 mutants were identified for essential oil yield and davanone content, respectively. Molecular diversity analysis of 16 high essential oil yielding M₂ plants and two check cultivars using ISSR markers revealed an average 79 % polymorphism, with the primer UBC-845 having the highest polymorphic information content. We identified several superior mutants including DM2158 (essential oil content-0.5 %) and DM2103 (Davanone content-70.92 %), indicating that mutation breeding can be an effective strategy to develop novel davana cultivars with higher essential oil and davanone content.

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.