[环状RNA-微RNA-信使RNA免疫调节网络在儿童过敏性哮喘中的作用]。

Q3 Medicine
Sai-Hua Huang, Jin-Tao Zhou, Yan Wang, Xiao Han
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引用次数: 0

摘要

目的:通过分析微阵列数据集,探讨儿童变应性哮喘中潜在的环状RNA (circRNA)-microRNA (miRNA)-信使RNA (mRNA)免疫调控网络。方法:使用GEO数据库获取过敏性哮喘患儿和健康对照的circRNA、miRNA和mRNA数据集。Limma包用于鉴定差异表达的circRNA (DEcircRNA)、miRNA (DEmiRNA)和mRNA (demmrna)。利用ENCORI等工具预测和构建内源性RNA调控网络。使用DAVID数据库进行GO和KEGG富集分析,使用CIBERSORT和Pearson识别与免疫细胞浸润相关的基因。结果:在哮喘组和对照组之间共鉴定出130个decircrna、40个demirna和802个demmrna,并建立了一个由12个circrna、7个mirna和75个mrna组成的调控网络。GO分析显示差异表达基因主要参与生长发育调控,KEGG分析显示差异表达基因主要参与mTOR信号通路。CIBERSORT分析显示,与对照组相比,哮喘组CD8+ T细胞和静息NK细胞百分比较高,而静息CD4+记忆T细胞和活化肥大细胞百分比较低。此外,Pearson相关分析确定了6个与免疫细胞浸润呈正相关的关键mrna。结论:本研究构建的ceRNA免疫调控网络为研究儿童变应性哮喘的发病机制和潜在的治疗靶点提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[The function of circular RNA-microRNA-messenger RNA immune regulatory network in childhood allergic asthma].

Objectives: To investigate the potential circular RNA (circRNA)-microRNA (miRNA)-messenger RNA (mRNA) immune regulatory network in childhood allergic asthma by analyzing microarray datasets.

Methods: GEO database was used to obtain the datasets of circRNA, miRNA, and mRNA from children with allergic asthma and healthy controls. The Limma package was used to identify differentially expressed circRNA (DEcircRNA), miRNA (DEmiRNA), and mRNA (DEmRNA). ENCORI and other tools were used to predict and construct the regulatory network of endogenous RNA. The DAVID database was used to perform GO and KEGG enrichment analyses, and CIBERSORT and Pearson were used to identify genes associated with immune cell infiltration.

Results: A total of 130 DEcircRNAs, 40 DEmiRNAs, and 802 DEmRNAs were identified between the asthma and control groups, and a regulatory network consisting of 12 circRNAs, 7 miRNAs, and 75 mRNAs was established. The GO analysis showed that the differentially expressed genes were mainly involved in the regulation of growth and development, and the KEGG analysis showed that they were mainly involved in the mTOR signaling pathway. The CIBERSORT analysis showed that compared with the control group, the asthma group had higher percentages of CD8+ T cells and resting NK cells and lower percentages of resting CD4+ memory T cells and activated mast cells. In addition, the Pearson correlation analysis identified six key mRNAs that were positively correlated with immune cell infiltration.

Conclusions: The ceRNA immune regulatory network constructed in this study provides a basis for research on the mechanism of childhood allergic asthma and potential therapeutic targets.

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来源期刊
中国当代儿科杂志
中国当代儿科杂志 Medicine-Pediatrics, Perinatology and Child Health
CiteScore
1.50
自引率
0.00%
发文量
5006
期刊介绍: The Chinese Journal of Contemporary Pediatrics (CJCP) is a peer-reviewed open access periodical in the field of pediatrics that is sponsored by the Central South University/Xiangya Hospital of Central South University and under the auspices of the Ministry of Education of China. It is cited as a source in the scientific and technological papers of Chinese journals, the Chinese Science Citation Database (CSCD), and is one of the core Chinese periodicals in the Peking University Library. CJCP has been indexed by MEDLINE/PubMed/PMC of the American National Library, American Chemical Abstracts (CA), Holland Medical Abstracts (EM), Western Pacific Region Index Medicus (WPRIM), Scopus and EBSCO. It is a monthly periodical published on the 15th of every month, and is distributed both at home and overseas. The Chinese series publication number is CN 43-1301/R;ISSN 1008-8830. The tenet of CJCP is to “reflect the latest advances and be open to the world”. The periodical reports the most recent advances in the contemporary pediatric field. The majority of the readership is pediatric doctors and researchers.
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