Y M Zhang, X Li, X X Jia, J Z Liu, W Q Li, J F Xuan, S Y Feng, Z H Sun, W Y Zhang
{"title":"[EF-hand结构域蛋白2在肝细胞癌中的生物学作用初步研究]。","authors":"Y M Zhang, X Li, X X Jia, J Z Liu, W Q Li, J F Xuan, S Y Feng, Z H Sun, W Y Zhang","doi":"10.3760/cma.j.cn112150-20250312-00198","DOIUrl":null,"url":null,"abstract":"<p><p>This study investigates the expression pattern and functional significance of EF-hand domain-containing protein 2 (EFHD2) in hepatocellular carcinoma (HCC), with particular focus on its regulatory effects on tumor proliferation, migration, and invasion. Cellular experimental study was completed from June 2024 to January 2025 in the Basic Laboratory of the General Hospital of Southern Theater Command. TCGA database to determine EFHD2 expression and its clinicopathological correlations. GSCA database to assess methylation patterns and immune infiltration. Model of transient overexpression and knockdown of EFHD2 was constructed in hepatocellular carcinoma cells Hep3B, then RT-qPCR and Western blot were applied to verify the transfection efficiency. CCK-8 and colony formation assays for proliferation assessment, Transwell chambers for migration/invasion quantification. Protein-protein interaction networks were constructed via STRING, followed by GO/KEGG enrichment analysis. Statistical analysis was performed using the two independent samples <i>t</i>-test. The results showed that EFHD2 demonstrated significant upregulation in HCC tissues versus normal controls (<i>P</i><0.05). Elevated EFHD2 expression correlated with advanced clinical stage (<i>P</i><0.05) and poor differentiation (<i>P</i><0.05). In the CCK-8 assay, the EFHD2 overexpression group demonstrated significantly higher cell viability than the control group, as evidenced by 450 nm relative absorbance values on Day 1 (0.529±0.019 <i>vs.</i> 0.515±0.016, <i>F</i>=0.041, <i>P</i>=0.320), Day 2 (1.356±0.019 <i>vs.</i> 1.094±0.042, <i>F</i>=3.833, <i>P</i><0.001), Day 3 (2.817±0.049 <i>vs.</i> 2.143±0.124, <i>F</i>=3.833, <i>P</i><0.001), and Day 4 (3.848±0.015 <i>vs.</i> 3.430±0.021, <i>F</i>=0.469, <i>P</i><0.001). The EFHD2 knockdown group showed reduced cell viability compared to controls: Day 1 (0.541±0.020 <i>vs.</i> 0.552±0.015, <i>F</i>=0.098, <i>P</i>=0.423), Day 2 (1.154±0.009 <i>vs.</i> 1.326±0.029, <i>F</i>=2.485, <i>P</i><0.001), Day 3 (2.453±0.041 <i>vs.</i> 2.653±0.031, <i>F</i>=0.479, <i>P</i><0.001), and Day 4 (3.685±0.038 <i>vs.</i> 3.836±0.021, <i>F</i>=6.804, <i>P</i><0.001). In colony formation assays, the overexpression group displayed a significant increase in colony numbers (254.667±23.861 <i>vs.</i> 186.000±16.703, <i>F</i>=0.865, <i>P</i>=0.015), whereas the knockdown group exhibited decreased colony formation (229.000±24.637 <i>vs.</i> 306.667±36.501, <i>F</i>=0.988, <i>P</i>=0.038). In Transwell assays, the EFHD2 overexpression group revealed enhanced migratory capacity [ (605.000±72.670) cells <i>vs.</i> (472.667±28.095) cells, <i>F</i>=2.462, <i>P</i>=0.042] and invasive potential [(767.333±21.221) cells <i>vs.</i> (414.333±16.623) cells, <i>F</i>=0.331, <i>P</i><0.001]. The knockdown group showed attenuated migration [(311.000±71.084) cells <i>vs.</i> (479.667±50.846) cells, <i>F</i>=0.718, <i>P</i>=0.029] and invasion [(247.667±48.263) cells <i>vs.</i> (345.667±32.130) cells, <i>F</i>=0.727, <i>P</i>=0.043] compared to controls. The network of EFHD2-interacting proteins was further constructed by the STRING database, and the GO and KEGG analysis were used to perform bioinformatics analysis reveal that EFHD2 is mainly involved in actin cytoskeleton regulation. In conclusion, EFHD2 is highly expressed in HCC and is involved in the process of proliferation, migration and invasion of HCC.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 8","pages":"1224-1231"},"PeriodicalIF":0.0000,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Preliminary study on the biological role of EF-hand domain-containing protein 2 in hepatocellular carcinoma].\",\"authors\":\"Y M Zhang, X Li, X X Jia, J Z Liu, W Q Li, J F Xuan, S Y Feng, Z H Sun, W Y Zhang\",\"doi\":\"10.3760/cma.j.cn112150-20250312-00198\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study investigates the expression pattern and functional significance of EF-hand domain-containing protein 2 (EFHD2) in hepatocellular carcinoma (HCC), with particular focus on its regulatory effects on tumor proliferation, migration, and invasion. Cellular experimental study was completed from June 2024 to January 2025 in the Basic Laboratory of the General Hospital of Southern Theater Command. TCGA database to determine EFHD2 expression and its clinicopathological correlations. GSCA database to assess methylation patterns and immune infiltration. Model of transient overexpression and knockdown of EFHD2 was constructed in hepatocellular carcinoma cells Hep3B, then RT-qPCR and Western blot were applied to verify the transfection efficiency. CCK-8 and colony formation assays for proliferation assessment, Transwell chambers for migration/invasion quantification. Protein-protein interaction networks were constructed via STRING, followed by GO/KEGG enrichment analysis. Statistical analysis was performed using the two independent samples <i>t</i>-test. The results showed that EFHD2 demonstrated significant upregulation in HCC tissues versus normal controls (<i>P</i><0.05). Elevated EFHD2 expression correlated with advanced clinical stage (<i>P</i><0.05) and poor differentiation (<i>P</i><0.05). In the CCK-8 assay, the EFHD2 overexpression group demonstrated significantly higher cell viability than the control group, as evidenced by 450 nm relative absorbance values on Day 1 (0.529±0.019 <i>vs.</i> 0.515±0.016, <i>F</i>=0.041, <i>P</i>=0.320), Day 2 (1.356±0.019 <i>vs.</i> 1.094±0.042, <i>F</i>=3.833, <i>P</i><0.001), Day 3 (2.817±0.049 <i>vs.</i> 2.143±0.124, <i>F</i>=3.833, <i>P</i><0.001), and Day 4 (3.848±0.015 <i>vs.</i> 3.430±0.021, <i>F</i>=0.469, <i>P</i><0.001). The EFHD2 knockdown group showed reduced cell viability compared to controls: Day 1 (0.541±0.020 <i>vs.</i> 0.552±0.015, <i>F</i>=0.098, <i>P</i>=0.423), Day 2 (1.154±0.009 <i>vs.</i> 1.326±0.029, <i>F</i>=2.485, <i>P</i><0.001), Day 3 (2.453±0.041 <i>vs.</i> 2.653±0.031, <i>F</i>=0.479, <i>P</i><0.001), and Day 4 (3.685±0.038 <i>vs.</i> 3.836±0.021, <i>F</i>=6.804, <i>P</i><0.001). In colony formation assays, the overexpression group displayed a significant increase in colony numbers (254.667±23.861 <i>vs.</i> 186.000±16.703, <i>F</i>=0.865, <i>P</i>=0.015), whereas the knockdown group exhibited decreased colony formation (229.000±24.637 <i>vs.</i> 306.667±36.501, <i>F</i>=0.988, <i>P</i>=0.038). In Transwell assays, the EFHD2 overexpression group revealed enhanced migratory capacity [ (605.000±72.670) cells <i>vs.</i> (472.667±28.095) cells, <i>F</i>=2.462, <i>P</i>=0.042] and invasive potential [(767.333±21.221) cells <i>vs.</i> (414.333±16.623) cells, <i>F</i>=0.331, <i>P</i><0.001]. The knockdown group showed attenuated migration [(311.000±71.084) cells <i>vs.</i> (479.667±50.846) cells, <i>F</i>=0.718, <i>P</i>=0.029] and invasion [(247.667±48.263) cells <i>vs.</i> (345.667±32.130) cells, <i>F</i>=0.727, <i>P</i>=0.043] compared to controls. The network of EFHD2-interacting proteins was further constructed by the STRING database, and the GO and KEGG analysis were used to perform bioinformatics analysis reveal that EFHD2 is mainly involved in actin cytoskeleton regulation. In conclusion, EFHD2 is highly expressed in HCC and is involved in the process of proliferation, migration and invasion of HCC.</p>\",\"PeriodicalId\":24033,\"journal\":{\"name\":\"中华预防医学杂志\",\"volume\":\"59 8\",\"pages\":\"1224-1231\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-08-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华预防医学杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/cma.j.cn112150-20250312-00198\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华预防医学杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/cma.j.cn112150-20250312-00198","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
[Preliminary study on the biological role of EF-hand domain-containing protein 2 in hepatocellular carcinoma].
This study investigates the expression pattern and functional significance of EF-hand domain-containing protein 2 (EFHD2) in hepatocellular carcinoma (HCC), with particular focus on its regulatory effects on tumor proliferation, migration, and invasion. Cellular experimental study was completed from June 2024 to January 2025 in the Basic Laboratory of the General Hospital of Southern Theater Command. TCGA database to determine EFHD2 expression and its clinicopathological correlations. GSCA database to assess methylation patterns and immune infiltration. Model of transient overexpression and knockdown of EFHD2 was constructed in hepatocellular carcinoma cells Hep3B, then RT-qPCR and Western blot were applied to verify the transfection efficiency. CCK-8 and colony formation assays for proliferation assessment, Transwell chambers for migration/invasion quantification. Protein-protein interaction networks were constructed via STRING, followed by GO/KEGG enrichment analysis. Statistical analysis was performed using the two independent samples t-test. The results showed that EFHD2 demonstrated significant upregulation in HCC tissues versus normal controls (P<0.05). Elevated EFHD2 expression correlated with advanced clinical stage (P<0.05) and poor differentiation (P<0.05). In the CCK-8 assay, the EFHD2 overexpression group demonstrated significantly higher cell viability than the control group, as evidenced by 450 nm relative absorbance values on Day 1 (0.529±0.019 vs. 0.515±0.016, F=0.041, P=0.320), Day 2 (1.356±0.019 vs. 1.094±0.042, F=3.833, P<0.001), Day 3 (2.817±0.049 vs. 2.143±0.124, F=3.833, P<0.001), and Day 4 (3.848±0.015 vs. 3.430±0.021, F=0.469, P<0.001). The EFHD2 knockdown group showed reduced cell viability compared to controls: Day 1 (0.541±0.020 vs. 0.552±0.015, F=0.098, P=0.423), Day 2 (1.154±0.009 vs. 1.326±0.029, F=2.485, P<0.001), Day 3 (2.453±0.041 vs. 2.653±0.031, F=0.479, P<0.001), and Day 4 (3.685±0.038 vs. 3.836±0.021, F=6.804, P<0.001). In colony formation assays, the overexpression group displayed a significant increase in colony numbers (254.667±23.861 vs. 186.000±16.703, F=0.865, P=0.015), whereas the knockdown group exhibited decreased colony formation (229.000±24.637 vs. 306.667±36.501, F=0.988, P=0.038). In Transwell assays, the EFHD2 overexpression group revealed enhanced migratory capacity [ (605.000±72.670) cells vs. (472.667±28.095) cells, F=2.462, P=0.042] and invasive potential [(767.333±21.221) cells vs. (414.333±16.623) cells, F=0.331, P<0.001]. The knockdown group showed attenuated migration [(311.000±71.084) cells vs. (479.667±50.846) cells, F=0.718, P=0.029] and invasion [(247.667±48.263) cells vs. (345.667±32.130) cells, F=0.727, P=0.043] compared to controls. The network of EFHD2-interacting proteins was further constructed by the STRING database, and the GO and KEGG analysis were used to perform bioinformatics analysis reveal that EFHD2 is mainly involved in actin cytoskeleton regulation. In conclusion, EFHD2 is highly expressed in HCC and is involved in the process of proliferation, migration and invasion of HCC.
期刊介绍：
Chinese Journal of Preventive Medicine (CJPM), the successor to Chinese Health Journal , was initiated on October 1, 1953. In 1960, it was amalgamated with the Chinese Medical Journal and the Journal of Medical History and Health Care , and thereafter, was renamed as People’s Care . On November 25, 1978, the publication was denominated as Chinese Journal of Preventive Medicine . The contents of CJPM deal with a wide range of disciplines and technologies including epidemiology, environmental health, nutrition and food hygiene, occupational health, hygiene for children and adolescents, radiological health, toxicology, biostatistics, social medicine, pathogenic and epidemiological research in malignant tumor, surveillance and immunization.
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