瘢痕疙瘩表皮内神经纤维和朗格汉斯细胞的三维分析，重点是瘙痒。

IF 3.9 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-08-19 DOI:10.1038/s41598-025-11674-8

Haruka Matsuzoe, Koh-Ei Toyoshima, Miki Takase, Ayako Tsuchiya, Mika Ikeda, Motoko Naitoh, Kazuya Kataoka, Tomoya Kawabata, Miho Ogawa, Naoki Morimoto, Takashi Tsuji

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引用次数: 0

摘要

瘢痕疙瘩是一种顽固性皮肤纤维化病变，可从原发病灶逐渐扩展到周围正常区域。在本研究中，我们利用三维免疫荧光分析胸前部和耳部瘢痕疙瘩的神经纤维和朗格汉斯细胞，并分析P物质作为瘙痒介质的信使rna表达，研究瘢痕疙瘩的不可控瘙痒以及瘢痕疙瘩症状与瘙痒的关系。胸前瘢痕疙瘩周围有大量神经纤维浸润表皮，有瘙痒的报道，其中一些延伸到颗粒层。然而，在瘙痒期，瘢痕疙瘩的位置或区域，朗格汉斯细胞没有差异。此外，我们还观察到P物质基因在前胸瘢痕疙瘩周围的表达。我们的观察性研究表明，瘢痕疙瘩前部病变的顽固性瘙痒、表皮内神经纤维密度增加、Th2细胞因子和P物质表达升高之间存在关联。

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Three-dimensional analysis of intraepidermal nerve fibres and Langerhans cells in keloids with a focus on pruritus.

Keloids are intractable dermato-fibrotic lesions that progressively expands from the primary lesion to the surrounding normal areas. In this study, we investigated uncontrollable pruritus in keloids and the association between keloid symptoms and pruritus using three-dimensional immunofluorescence for the analysis of nerve fibres and Langerhans cells in the anterior chest and ear keloids, and the analysis of messenger-RNA expression of substance P as a pruritus mediator. Nerve fibres infiltrating the epidermis were numerous in the periphery of the anterior chest keloids, where pruritus was reported, with some extending into the granular layer. However, there was no difference in Langerhans cells during pruritus, location or region of the keloid. Furthermore, we observed substance P gene expression in the periphery of the anterior chest keloids. Our observational study suggests an association between intractable pruritus in anterior keloid lesions, increased intraepidermal nerve fibre density, and elevated expression of Th2 cytokines and substance P.

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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