Eric Fabrício Marialva, Keillen Monick Martins-Campos, Victor Ramos de Almeida, Claudia María Ríos-Velasquez, Antônio Jorge Tempone, Felipe Arley Costa Pessoa, Yara Maria Traub-Cseko
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For instance, populations of Ny. umbratilis from the Manacapuru municipality (MAN), located on the south bank of the Negro river, in the Amazonas state of Brazil, shows refractoriness to Leishmania infection, while populations from Rio Preto da Eva municipality (RPE), located on the north bank of the Negro river, are susceptible to infection. This lack of vectorial capacity may be caused by several factors, including the intestinal bacterial microbiota of sand flies.</p><p><strong>Methods: </strong>In this work, we carried out a metagenomic study of the intestinal microbiota of Ny. umbratilis populations from MAN and RPE. Ny. umbratilis females were collected in forested areas, sand fly midguts were dissected, DNA was extracted, and the 16 S rRNA gene sequenced to identify the bacterial composition of the microbiota.</p><p><strong>Results: </strong>In total, 16 phyla, 33 classes, 49 orders, 93 families, and 112 genera of bacteria were identified. The phylum Proteobacteria was the most frequent (85.9%) in both localities, followed by the phyla Bacteroidetes, Actinobacteria, and Firmicutes with, 9.9%, 4.9%, and 4.4%, respectively. In MAN, 84 genera were identified and 79 in RPE, with MAN having a greater richness compared with RPE. Among these, the genera Rickettsia, Prevotella, Porphyromonas, Peptostreptococcus, and Caulobacter were the most prevalent in MAN, and the genera Rickettsia, Prevotella, Cryocola, Porphyromonas, and Caulobacter were the most prevalent in RPE.</p><p><strong>Conclusions: </strong>Bacterial microbiota from MAN insects presents a greater diversity in relation to the RPE insects. Some of the identified bacteria have the potential to be used in alternative transmission control approaches as the development of transgenic vectors, and also, bacteria found exclusively in MAN sand flies may be candidates for a future transmission control approach to combat leishmaniasis in the Amazon region.</p>","PeriodicalId":19793,"journal":{"name":"Parasites & Vectors","volume":"18 1","pages":"354"},"PeriodicalIF":3.5000,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12366322/pdf/","citationCount":"0","resultStr":"{\"title\":\"Investigation of bacterial microbiota variability in two allopatric populations of Nyssomyia umbratilis, susceptible and nonsusceptible to Leishmania (Viannia) guyanensis infection in the Amazon region.\",\"authors\":\"Eric Fabrício Marialva, Keillen Monick Martins-Campos, Victor Ramos de Almeida, Claudia María Ríos-Velasquez, Antônio Jorge Tempone, Felipe Arley Costa Pessoa, Yara Maria Traub-Cseko\",\"doi\":\"10.1186/s13071-025-06976-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Sand flies of the species Nyssomyia umbratilis (Diptera: Psychodidae: Phlebotominae) are vectors of the parasite Leishmania (Viannia) guyanensis, one of the etiological agents of cutaneous leishmaniasis in the Amazon region. 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引用次数: 0
摘要
背景:亚马逊地区皮肤利什曼病病原之一利什曼原虫(Viannia)的传播媒介为黑纹沙蝇(双翅目:精神蛉科:白蛉科)。此外,纽约。黑曲霉是一种隐种,不同种群在传播寄生虫的能力上存在差异。例如,纽约的人口。位于巴西亚马逊州内格罗河南岸的Manacapuru市(MAN)的umbratilis对利什曼原虫感染表现出耐药性,而位于内格罗河北岸的bb0 Preto da Eva市(RPE)的人群易受感染。这种媒介能力的缺乏可能是由几个因素造成的,包括沙蝇的肠道细菌微生物群。方法:在本工作中,我们对Ny的肠道微生物群进行了宏基因组研究。来自MAN和RPE的umbratilis种群。纽约。在林区采集云母,解剖沙蝇中肠,提取DNA,测序16s rRNA基因,鉴定菌群细菌组成。结果:共鉴定出细菌16门33纲49目93科112属。变形菌门(85.9%)最多,拟杆菌门(9.9%)、放线菌门(4.9%)和厚壁菌门(4.4%)次之。MAN中鉴定出84个属,RPE中鉴定出79个属,MAN的丰富度高于RPE。其中MAN以立克次体属、普雷沃氏菌属、卟啉单胞菌属、胃链球菌属、Caulobacter属最常见,RPE以立克次体属、普雷沃氏菌属、Cryocola属、卟啉单胞菌属、Caulobacter属最常见。结论:与RPE昆虫相比,MAN昆虫的细菌微生物群具有更大的多样性。随着转基因载体的发展,一些已鉴定的细菌有可能用于替代传播控制方法,而且,仅在MAN沙蝇中发现的细菌可能是未来对抗亚马逊地区利什曼病传播控制方法的候选国。
Investigation of bacterial microbiota variability in two allopatric populations of Nyssomyia umbratilis, susceptible and nonsusceptible to Leishmania (Viannia) guyanensis infection in the Amazon region.
Background: Sand flies of the species Nyssomyia umbratilis (Diptera: Psychodidae: Phlebotominae) are vectors of the parasite Leishmania (Viannia) guyanensis, one of the etiological agents of cutaneous leishmaniasis in the Amazon region. In addition, Ny. umbratilis is a cryptic species, with populations showing differences in their ability to transmit the parasite. For instance, populations of Ny. umbratilis from the Manacapuru municipality (MAN), located on the south bank of the Negro river, in the Amazonas state of Brazil, shows refractoriness to Leishmania infection, while populations from Rio Preto da Eva municipality (RPE), located on the north bank of the Negro river, are susceptible to infection. This lack of vectorial capacity may be caused by several factors, including the intestinal bacterial microbiota of sand flies.
Methods: In this work, we carried out a metagenomic study of the intestinal microbiota of Ny. umbratilis populations from MAN and RPE. Ny. umbratilis females were collected in forested areas, sand fly midguts were dissected, DNA was extracted, and the 16 S rRNA gene sequenced to identify the bacterial composition of the microbiota.
Results: In total, 16 phyla, 33 classes, 49 orders, 93 families, and 112 genera of bacteria were identified. The phylum Proteobacteria was the most frequent (85.9%) in both localities, followed by the phyla Bacteroidetes, Actinobacteria, and Firmicutes with, 9.9%, 4.9%, and 4.4%, respectively. In MAN, 84 genera were identified and 79 in RPE, with MAN having a greater richness compared with RPE. Among these, the genera Rickettsia, Prevotella, Porphyromonas, Peptostreptococcus, and Caulobacter were the most prevalent in MAN, and the genera Rickettsia, Prevotella, Cryocola, Porphyromonas, and Caulobacter were the most prevalent in RPE.
Conclusions: Bacterial microbiota from MAN insects presents a greater diversity in relation to the RPE insects. Some of the identified bacteria have the potential to be used in alternative transmission control approaches as the development of transgenic vectors, and also, bacteria found exclusively in MAN sand flies may be candidates for a future transmission control approach to combat leishmaniasis in the Amazon region.
期刊介绍:
Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish.
Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.