埃塞俄比亚蜂蜜酒乳酸菌的生物技术潜力研究。

IF 3.2 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2025-08-11 eCollection Date: 2025-01-01 DOI:10.1155/ijm/4014096
Minyilal Sisay, Asnake Desalegn, Fitsum Tigu, Mogessie Ashenafi, Feng-Yan Bai, Dagim Jirata Birri
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引用次数: 0

摘要

乳酸菌(LAB)的一些成员已被用作益生菌。埃塞俄比亚蜂蜜酒,Tej,可能是潜在益生菌的有用来源。然而,这种来源的实验室尚未对其益生菌特性进行评估。本研究对埃塞俄比亚蜂蜜酒中乳酸菌的体外益生菌特性进行了研究。为此,从埃塞俄比亚西南部采集了30个样本。首先测试了LAB分离物的抗菌活性,并对具有这种特性的分离物的益生菌特性进行了评估,如对酸、盐和胆汁的耐受性、粘附性、抗胆固醇活性、抗氧化活性和抗生素敏感性。采用琼脂孔扩散法测定菌株对试验菌的抑菌活性。用平板计数法评估酸、盐和胆汁耐受性。通过测定细菌对非极性溶剂对二甲苯的疏水性来评价其粘附性能。通过测定废肉汤中剩余的胆固醇来测定抗胆固醇作用。采用1,1-二苯基-2-苦味肼基自由基清除能力评价菌株的抗氧化能力,采用纸片扩散法检测菌株的抗生素敏感性。从Tej样品中共分离到143个实验室。LAB计数在7.4 ~ 6.5 log cfu/mL之间。143株乳酸菌中,37株表现出不同程度的抑菌活性。其中8种经16S核糖体基因序列分析鉴定达到种水平。对博氏志贺氏菌ATCC 25931的抑制作用最大,为19±4.2 mm,对大肠杆菌ATTC 25922和金黄色葡萄球菌ATTC 25913的抑制作用最小,为8.0±1.4 mm。除2株外,其余菌株在pH 2和pH 3条件下均存活(18.4% ~ 89.6%)。37株存活于50%以上的胆汁和54%-67%的粘附能力。降低胆固醇和清除1,1-二苯基-2-苦酰肼自由基的能力分别为8% ~ 54%和13% ~ 33%。大多数分离株对抗生素敏感,除了一株对所有测试的抗生素都耐药。本研究表明,从埃塞俄比亚蜂蜜酒Tej中分离的许多实验室具有益生菌特性,可作为益生菌的候选物。我们建议对乳酸菌分离物的体内益生菌特性进行评估,以提供强有力的支持证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Biotechnological Potential of Lactic Acid Bacteria Isolated From Ethiopian Honey Wine, <i>Tej</i>.

Biotechnological Potential of Lactic Acid Bacteria Isolated From Ethiopian Honey Wine, Tej.

Some members of the lactic acid bacteria (LAB) have been used as probiotics. Ethiopian honey wine, Tej, may be a useful source of potential probiotic bacteria. However, LABs from this source have not yet been evaluated for their probiotic properties. This study was conducted to evaluate the in vitro probiotic properties of LAB isolated from Ethiopian honey wine, Tej. For this purpose, 30 samples were collected from Southwest Ethiopia. LAB isolates were first tested for their antimicrobial activity and those with this property were evaluated for probiotic properties, such as tolerance to acid, salt, and bile, adherence properties, anticholesterol activity, antioxidant activity, and antibiotic susceptibility. The antibacterial activity of the LAB isolates against test organisms was assessed by the agar well diffusion method. Acid, salt, and bile tolerance were evaluated by the plate count method. Adhesion properties were assessed by the determination of bacterial hydrophobicity to the nonpolar solvent p-xylene. Anticholesterol was determined by measuring the remaining cholesterol in the spent broth. The antioxidant capacity was assessed by the 1,1-diphenyl-2-picrylhydrazyl free radical-scavenging capacity, and the antibiotic susceptibility of isolates was tested by the disk diffusion method. A total of 143 LABs were isolated from Tej samples. The LAB count was ranged between 7.4 and 6.5 log cfu/mL. Of the 143 LAB isolates, 37 exhibited different levels of antimicrobial activity. Eight of these were identified to species level by 16S ribosomal genes sequence analysis. The greatest inhibition was against Shigella boydii ATCC 25931 with 19 ± 4.2 mm, and the least inhibitory activity was against Escherichia coli ATTC 25922 and Staphylococcus aureus ATTC 25913 with 8.0 ± 1.4. All except two isolates survived at pH 2 and pH 3 (18.4%-89.6%). Then, 37 isolates survived in more than 50% bile and 54%-67% adhesion capacity. The cholesterol-lowering and 1,1-diphenyl-2-picrylhydrazyl free radical-scavenging capacities ranged from 8% to 54% and 13% to 33%, respectively. Most isolates were susceptible to antibiotics, except for one isolate that resisted all tested antibiotics. This study shows that many LABs isolated from Ethiopian honey wine, Tej have probiotic properties and they can be considered as probiotic candidates. We recommend evaluation of in vivo probiotic properties of the LAB isolates to provide strong supporting evidence.

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来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
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